The Brazilian cerrado has a great diversity of plant species with power medicinal
potential. Essential oils are natural substances with distinct smells, expelled by
specialized glands of aromatic plants, with a chemical structure basically made up of
carbon, hydrogen and oxygen, having composition distinct components that characterize
the pleasant aroma. The extraction and characterization of essential oils from native
species can contribute to understanding this potential. The objective of this study was to
collect, extract and characterize the EOs of Citrus species native in Cerrado region of
Tocantins. Three collections of fresh Citrus sp leaves and one collection of the fruit were
made at the end of the rainy season, in May and June 2023, in the city of Araguaína, with
the following geographical coordinates 7°10'54.2"S 48°11'51.0"W. The EO was extracted
using steam distillation coupled to a Clevenger system, with an average yield of 0.17%.
The chemical constituents of the EO were characterized by gas chromatography coupled
with GC/MS mass spectrometry. The following components were obtained: pinene; D-
limonene; Eucalyptol; δ-3-carene; terpinolene; γ-terpinene; linalool; isopulegol, from the
extractions of the fresh leaves of Citrus sp. The components obtained from the extraction
of the fruit: 3- Carene; α-Fellandrene; Pinene; o-Cymene; D-limonene; γ-terpinene.
Furthermore, to expand our knowledge in mass spectrometry, we proposed
fragmentations for peaks with greater relative abundance.
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