Detecção de brucella ovis, actinobacillus seminis e histophilus somni no sêmen de reprodutores ovinos no estado do Tocantins através de ferramenta molecular
Infectious ovine epididymitis occurs worldwide and causes major economic losses to affect infected breeding males fertility and flocks productivity. The most common causative agents are Brucella ovis, Actinobacillus seminis and Histophilus somni. Clinical alterations (epididymitis and orchitis) a...
| Autor principal: | Santos, Ruth Martins |
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| Idioma: | pt_BR |
| Publicado em: |
2024
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| Assuntos: | |
| Acesso em linha: |
http://hdl.handle.net/11612/7074 |
| Resumo: |
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Infectious ovine epididymitis occurs worldwide and causes major economic losses to
affect infected breeding males fertility and flocks productivity. The most common
causative agents are Brucella ovis, Actinobacillus seminis and Histophilus somni.
Clinical alterations (epididymitis and orchitis) are related to the presence of
neutrophils in semen, reduced concentration and motility and sperm non-viability,
with subfertility and infertility being the main consequences of disease and the main
causes of economic losses. In this context, PCR has been proposed as a definitive
diagnostic method and can even be designed to simultaneously detect more than
one causative agent in a single reaction. The aim of this study was to detect the main
causative agents of infectious ovine epididymitis in the semen of breeding animals
from 21 properties in Tocantins state using multiplex species-specific PCR. The
detection frequencies of B. ovis (50.0%), A. seminis (29.5%) and H. somni (27.2%)
by multiplex PCR were compared by Fisher's Exact Test, using GraphPad Prisma 5.0
software. B. ovis was the most frequent agent in animals with alterations
characteristic of the disease. |