Acompanhamento da microbiota indicadora e patogênica durante a shelf life de longissimus dorsi embalado à vácuo
Brazil is one of the largest meat producers in the world. It had an estimated production of 9.7 million tons of beef in 2022, of which 2.4 million tons were exported, whth invoicing of US$ 1.3 billion. Meat, like any product of animal origin, is a food that requires care with microbial pathogens,...
| Autor principal: | Mendonça, Jeycy Kelle Sirqueira |
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| Idioma: | pt_BR |
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2024
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http://hdl.handle.net/11612/7076 |
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ir-11612-70762024-10-26T06:03:16Z Acompanhamento da microbiota indicadora e patogênica durante a shelf life de longissimus dorsi embalado à vácuo Mendonça, Jeycy Kelle Sirqueira Ribeiro Júnior, José Carlos Análises microbiológicas. Carne bovina resfriada. Doenças Transmitidas por Alimentos. Qualidade. CNPQ::CIENCIAS AGRARIAS Brazil is one of the largest meat producers in the world. It had an estimated production of 9.7 million tons of beef in 2022, of which 2.4 million tons were exported, whth invoicing of US$ 1.3 billion. Meat, like any product of animal origin, is a food that requires care with microbial pathogens, as animals can be natural reservoirs. Aiming at longer shelf life and preserving the quality of the meat, technologies are applied to the fresh product, such as vacuum packaging, which also contributes to the wet maturation stage. The objective of this work was to monitor the indicator and pathogenic microbiota indicator aduring the shelf life of vacuum-packed bovine Longissimus dorsi through microbiological and biomolecular analyses. Five samples of vacuum-packed beef tenderloin (Longissimus dorsi) were collected. Each of the samples was divided into four parts and each part was used to compose a part of each of the pools, totaling four pools kept at 7oC that were analyzed on days 0, 20, 40 and 60 after primary packaging. The microorganisms searched were mesophilic aerobes, psychrotrophs, Pseudomonas spp., coliforms at 30°C, enterobacteria, coagulase-positive Staphylococcus, Escherichia coli shiga toxin-producing (STEC), enteropathogenic (EPEC), enterohemorrhagic (EHEC), enteroaggregative (EAEC ), enterotoxigenic (ETEC) and enteroinvasive (EIEC), Salmonella spp. and Listeria monocytogenes. Counts of indicator microorganisms increased progressively in most of the evaluated groups. All pathogenic microorganisms searched were identified. Of the microorganisms that have a standard defined in legislation, Salmonella spp. and E. coli were outside the standard defined by legislation from the 20th. Even with some standards not defined by law, other groups of indicator microorganisms, with recognized proteolytic and/or lipolytic capacity, which presented high counts, it is possible that the analyzed product presents changes in quality and safety for consumption that can shorten the shelf life, at the same time that it can be a vehicle for pathogens directly and indirectly related to contamination that predisposes foodborne illnesses. For to increase the shelf life of the vacuum-packed sirloin beef and compliance the standards of quality and microbiological safety, it is necessary for the establishment to carry out revisions and adjustments in the execution and monitoring of self-control programs to guarantee the quality of the products. O Brasil é um dos maiores produtores de carne do mundo. Teve produção estimada em 9,7 milhões de toneladas de carne bovina no ano de 2022, das quais foram exportadas 2,4 milhões de toneladas, que renderam um faturamento de US$ 1,3 bilhões. A carne, como todo produto de origem animal, é um alimento que requer cuidado com os patógenos microbianos, pois os animais podem ser reservatórios naturais. Visando maior prazo de validade e preservação da qualidade da carne, tecnologias são aplicadas no produto in natura, como a embalagem a vácuo, que também contribui para a etapa de maturação úmida. O objetivo do trabalho foi acompanhar a microbiota indicadora e patogênica durante a shelf life de Longissimus dorsi bovino embalado a vácuo através de análises microbiológicas e biomoleculares. Foram coletadas cinco amostras do corte cárneo bovino contrafilé (Longissimus dorsi) embalados a vácuo. Cada uma das amostras foi fracionada em quatro peças e cada peça foi utilizada para compor uma parte de cada um dos pools, totalizando quatro pools mantidos à 7oC que foram analisados nos dias 0, 20, 40 e 60 após a embalagem primária. Os micro-organismos pesquisados foram aeróbios mesófilos, psicrotróficos, Pseudomonas spp., coliformes a 30°C, enterobactérias, Staphylococcus coagulase positiva, Escherichia coli produtora da toxina shiga (STEC), enteropatogênica (EPEC), enterohemorrágica (EHEC), enteroagregativa (EAEC), enterotoxigênica (ETEC) e enteroinvasiva (EIEC), Salmonella spp. e Listeria monocytogenes. As contagens dos micro-organismos indicadores foram aumentando progressivamente na maioria dos grupos avaliados. Todos os microrganismos patogênicos pesquisados foram identificados. Dos micro-organismos que possuem padrão definido em legislação, Salmonella spp. e E. coli se apresentaram fora do padrão definido por legislação a partir do dia 20. Mesmo com alguns padrões não definidos em lei, outros grupos de microrganismos indicadores, com reconhecida capacidade proteolítica e/ou lipolítica, que apresentaram-se com elevadas contagens, é possível que o produto analisado apresente alterações de qualidade e segurança para o consumo que podem encurtar a shelf life, ao mesmo tempo de poder ser veículo de patógenos direta e indiretamente relacionados com contaminação que predispõe doenças transmitidas por alimentos. Para aumento da vida útil do contra-filé embalado a vácuo e atendimento dos padrões de qualidade e segurança microbiológica, faz-se necessário que o estabelecimento realize revisões e adequações na execução e no monitoramento dos programas de autocontrole para garantir a qualidade dos produtos. 2024-10-25T11:49:35Z 2024-10-25T11:49:35Z 2023 MENDONÇA, Jeycy Kelle Sirqueira .Acompanhamento da microbiota indicadora e patogênica durante a shelf life de longissimus dorsi embalado à vácuo. 2023. 55f. Dissertação – Universidade Federal do Tocantins, Programa de Pós-graduação em Sanidade animal e Saúde pública nos trópicos, Araguaína, 2023. http://hdl.handle.net/11612/7076 pt_BR application/pdf |
| institution |
Repositório Institucional - Universidade Federal do Tocantins - UFT |
| collection |
RepositorioUFT |
| language |
pt_BR |
| topic |
Análises microbiológicas. Carne bovina resfriada. Doenças Transmitidas por Alimentos. Qualidade. CNPQ::CIENCIAS AGRARIAS |
| spellingShingle |
Análises microbiológicas. Carne bovina resfriada. Doenças Transmitidas por Alimentos. Qualidade. CNPQ::CIENCIAS AGRARIAS Mendonça, Jeycy Kelle Sirqueira Acompanhamento da microbiota indicadora e patogênica durante a shelf life de longissimus dorsi embalado à vácuo |
| topic_facet |
Análises microbiológicas. Carne bovina resfriada. Doenças Transmitidas por Alimentos. Qualidade. CNPQ::CIENCIAS AGRARIAS |
| description |
Brazil is one of the largest meat producers in the world. It had an estimated production of 9.7
million tons of beef in 2022, of which 2.4 million tons were exported, whth invoicing of US$
1.3 billion. Meat, like any product of animal origin, is a food that requires care with microbial
pathogens, as animals can be natural reservoirs. Aiming at longer shelf life and preserving the
quality of the meat, technologies are applied to the fresh product, such as vacuum packaging,
which also contributes to the wet maturation stage. The objective of this work was to monitor
the indicator and pathogenic microbiota indicator aduring the shelf life of vacuum-packed
bovine Longissimus dorsi through microbiological and biomolecular analyses. Five samples
of vacuum-packed beef tenderloin (Longissimus dorsi) were collected. Each of the samples
was divided into four parts and each part was used to compose a part of each of the pools,
totaling four pools kept at 7oC that were analyzed on days 0, 20, 40 and 60 after primary
packaging. The microorganisms searched were mesophilic aerobes, psychrotrophs,
Pseudomonas spp., coliforms at 30°C, enterobacteria, coagulase-positive Staphylococcus,
Escherichia coli shiga toxin-producing (STEC), enteropathogenic (EPEC), enterohemorrhagic
(EHEC), enteroaggregative (EAEC ), enterotoxigenic (ETEC) and enteroinvasive (EIEC),
Salmonella spp. and Listeria monocytogenes. Counts of indicator microorganisms increased
progressively in most of the evaluated groups. All pathogenic microorganisms searched were
identified. Of the microorganisms that have a standard defined in legislation, Salmonella spp.
and E. coli were outside the standard defined by legislation from the 20th. Even with some
standards not defined by law, other groups of indicator microorganisms, with recognized
proteolytic and/or lipolytic capacity, which presented high counts, it is possible that the
analyzed product presents changes in quality and safety for consumption that can shorten the
shelf life, at the same time that it can be a vehicle for pathogens directly and indirectly related
to contamination that predisposes foodborne illnesses. For to increase the shelf life of the
vacuum-packed sirloin beef and compliance the standards of quality and microbiological
safety, it is necessary for the establishment to carry out revisions and adjustments in the
execution and monitoring of self-control programs to guarantee the quality of the products. |
| author_additional |
Ribeiro Júnior, José Carlos |
| author_additionalStr |
Ribeiro Júnior, José Carlos |
| author |
Mendonça, Jeycy Kelle Sirqueira |
| title |
Acompanhamento da microbiota indicadora e patogênica durante a shelf life de longissimus dorsi embalado à vácuo |
| title_short |
Acompanhamento da microbiota indicadora e patogênica durante a shelf life de longissimus dorsi embalado à vácuo |
| title_full |
Acompanhamento da microbiota indicadora e patogênica durante a shelf life de longissimus dorsi embalado à vácuo |
| title_fullStr |
Acompanhamento da microbiota indicadora e patogênica durante a shelf life de longissimus dorsi embalado à vácuo |
| title_full_unstemmed |
Acompanhamento da microbiota indicadora e patogênica durante a shelf life de longissimus dorsi embalado à vácuo |
| title_sort |
acompanhamento da microbiota indicadora e patogênica durante a shelf life de longissimus dorsi embalado à vácuo |
| publishDate |
2024 |
| url |
http://hdl.handle.net/11612/7076 |
| _version_ |
1816126038330048512 |
| score |
11.755432 |