IL-2, IL-5, TNF-α and IFN-γ mRNA expression in epidermal keratinocytes of systemic lupus erythematosus skin lesions
OBJECTIVE: To analyze cytokine gene expression in keratinocytes from patients with systemic lupus erythematosus (SLE). INTRODUCTION: Keratinocytes represent 95% of epidermal cells and can secrete several cytokines. METHODS: Keratinocytes were obtained by laser microdissection from 21 patients wi...
| Autor principal: | CARNEIRO, José Ronaldo Matos |
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| Outros Autores: | FUZII, Hellen Thais, KAYSER, Cristiane, ALBERTO, Fernando Lopes, SOARES, Fernando Augusto, SATO, Emília Inoue, ANDRADE, Luis Eduardo Coelho |
| Grau: | Artigo |
| Idioma: | eng |
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2011
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http://www.repositorio.ufpa.br:8080/jspui/handle/2011/2361 |
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ir-2011-23612018-03-08T16:36:28Z IL-2, IL-5, TNF-α and IFN-γ mRNA expression in epidermal keratinocytes of systemic lupus erythematosus skin lesions CARNEIRO, José Ronaldo Matos FUZII, Hellen Thais KAYSER, Cristiane ALBERTO, Fernando Lopes SOARES, Fernando Augusto SATO, Emília Inoue ANDRADE, Luis Eduardo Coelho Citocinas Interleucina Lúpus Eritematoso Sistêmico (LES) Expressão gênica OBJECTIVE: To analyze cytokine gene expression in keratinocytes from patients with systemic lupus erythematosus (SLE). INTRODUCTION: Keratinocytes represent 95% of epidermal cells and can secrete several cytokines. METHODS: Keratinocytes were obtained by laser microdissection from 21 patients with SLE (10 discoid and 11 acute lesions) at involved and uninvolved sites. All patients were receiving a low/moderate prednisone dose and 18 were receiving chloroquine diphosphate. IL-2, IL-5, TNF-α and IFN-γ gene expression was evaluated by real-time PCR and expressed as the ratio (R) to a pool of skin samples from 12 healthy volunteers. RESULTS: Heterogeneity in cytokine gene expression was found among patients with SLE. Eighteen of 38 valid SLE samples (47%) presented overexpression (R>1) of at least one cytokine. Lesional skin samples tended to show higher cytokine expression than samples from uninvolved skin (p = 0.06). IL-5 and IFN-γ were the most commonly overexpressed cytokines. Samples with cytokine overexpression corresponded to more extensive and severe lesions. Prednisone dose did not differ between samples without cytokine overexpression (15.71±3.45 mg/day) and those with overexpressed cytokines (12.68±5.41 mg/day) (p = 0.216). Samples from all patients not receiving diphosphate chloroquine had at least one overexpressed cytokine. CONCLUSIONS: The heterogeneous keratinocyte cytokine gene expression reflects the complex immunological and inflammatory background in SLE. Patients with severe/extensive skin lesions showed a higher frequency of cytokine gene overexpression. Increased IFN-γ and IL-5 expression suggests that Th1 and Th2 cells are involved in SLE skin inflammation. The possibility that prednisone and antimalarial drugs may have contributed to low cytokine gene expression in some samples cannot be ruled out. 2011-11-01T16:50:55Z 2011-11-01T16:50:55Z 2011 Artigo de Periódico CARNEIRO, José Ronaldo Matos et al. IL-2, IL-5, TNF-α and IFN-γ mRNA expression in epidermal keratinocytes of systemic lupus erythematosus skin lesions. Clinics, São Paulo, v. 66, n. 1, p. 77-82, 2011. Disponível em: <http://www.scielo.br/pdf/clin/v66n1/v66n1a14.pdf>. Acesso em: 01 nov. 2011. http://dx.doi.org/10.1590/S1807-59322011000100014. 1807-5932 http://www.repositorio.ufpa.br:8080/jspui/handle/2011/2361 eng Acesso Aberto application/pdf |
| institution |
Repositório Institucional - Universidade Federal do Pará |
| collection |
RI-UFPA |
| language |
eng |
| topic |
Citocinas Interleucina Lúpus Eritematoso Sistêmico (LES) Expressão gênica |
| spellingShingle |
Citocinas Interleucina Lúpus Eritematoso Sistêmico (LES) Expressão gênica CARNEIRO, José Ronaldo Matos IL-2, IL-5, TNF-α and IFN-γ mRNA expression in epidermal keratinocytes of systemic lupus erythematosus skin lesions |
| topic_facet |
Citocinas Interleucina Lúpus Eritematoso Sistêmico (LES) Expressão gênica |
| description |
OBJECTIVE: To analyze cytokine gene expression in keratinocytes from patients with systemic lupus erythematosus (SLE).
INTRODUCTION: Keratinocytes represent 95% of epidermal cells and can secrete several cytokines.
METHODS: Keratinocytes were obtained by laser microdissection from 21 patients with SLE (10 discoid and 11 acute lesions) at involved and uninvolved sites. All patients were receiving a low/moderate prednisone dose and 18 were receiving chloroquine diphosphate. IL-2, IL-5, TNF-α and IFN-γ gene expression was evaluated by real-time PCR and expressed as the ratio (R) to a pool of skin samples from 12 healthy volunteers.
RESULTS: Heterogeneity in cytokine gene expression was found among patients with SLE. Eighteen of 38 valid SLE samples (47%) presented overexpression (R>1) of at least one cytokine. Lesional skin samples tended to show higher cytokine expression than samples from uninvolved skin (p = 0.06). IL-5 and IFN-γ were the most commonly overexpressed cytokines. Samples with cytokine overexpression corresponded to more extensive and severe lesions. Prednisone dose did not differ between samples without cytokine overexpression (15.71±3.45 mg/day) and those with overexpressed cytokines (12.68±5.41 mg/day) (p = 0.216). Samples from all patients not receiving diphosphate chloroquine had at least one overexpressed cytokine.
CONCLUSIONS: The heterogeneous keratinocyte cytokine gene expression reflects the complex immunological and inflammatory background in SLE. Patients with severe/extensive skin lesions showed a higher frequency of cytokine gene overexpression. Increased IFN-γ and IL-5 expression suggests that Th1 and Th2 cells are involved in SLE skin inflammation. The possibility that prednisone and antimalarial drugs may have contributed to low cytokine gene expression in some samples cannot be ruled out. |
| format |
Artigo |
| author |
CARNEIRO, José Ronaldo Matos |
| author2 |
FUZII, Hellen Thais KAYSER, Cristiane ALBERTO, Fernando Lopes SOARES, Fernando Augusto SATO, Emília Inoue ANDRADE, Luis Eduardo Coelho |
| author2Str |
FUZII, Hellen Thais KAYSER, Cristiane ALBERTO, Fernando Lopes SOARES, Fernando Augusto SATO, Emília Inoue ANDRADE, Luis Eduardo Coelho |
| title |
IL-2, IL-5, TNF-α and IFN-γ mRNA expression in epidermal keratinocytes of systemic lupus erythematosus skin lesions |
| title_short |
IL-2, IL-5, TNF-α and IFN-γ mRNA expression in epidermal keratinocytes of systemic lupus erythematosus skin lesions |
| title_full |
IL-2, IL-5, TNF-α and IFN-γ mRNA expression in epidermal keratinocytes of systemic lupus erythematosus skin lesions |
| title_fullStr |
IL-2, IL-5, TNF-α and IFN-γ mRNA expression in epidermal keratinocytes of systemic lupus erythematosus skin lesions |
| title_full_unstemmed |
IL-2, IL-5, TNF-α and IFN-γ mRNA expression in epidermal keratinocytes of systemic lupus erythematosus skin lesions |
| title_sort |
il-2, il-5, tnf-α and ifn-γ mrna expression in epidermal keratinocytes of systemic lupus erythematosus skin lesions |
| publishDate |
2011 |
| url |
http://www.repositorio.ufpa.br:8080/jspui/handle/2011/2361 |
| _version_ |
1787149110823878656 |
| score |
11.674684 |