Sexually Transmitted Diseases (STD) are widespread public health concern, majority among adolescents, because they are most susceptible in respect of the sexuality. Early sexual initiation, multiple sex partners, and lack of condom use are considered risk factors and contribute to the increase in the number of STD cases. In order to better understand high-risk sexual behavior among students, a crosssectional study based on a self-answered anonymous questionnaire was conducted in Joaquim Vicente Rondon High School, a public school of Porto Velho city, Brazil. To verify STD's prevalence, samples of blood, urethral and vaginal discharge had been analyzed. A total of 122 students aged 11 to 19 years participated in the study. 84% known what is a STD, 82,8% informed always using condoms during sex, 11,5% didn't make use of condom, and 5,7% selected their sexual partner. To identify STD variety an enzyme-linked immunosorbent assay (ELISA test) and bacterioscopia by method of gram had been applied. 83 blood samples had been analyzed by ELISA test, and 41 smear tests had been stained by method of gramo The incidence to Chlamydia was observed in 65,3% of females, and 34,6% of young men. Gardnerella vaginalis, Candida albicans and Trichomonas vaginalis were more common pathogenic microorganisms found.
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