Artigo

Cinética da maturação nuclear in vitro de oócitos bubalinos

To evaluate the kinetics of the in vitro maturation of buffalo oocytes, 1.619 oocytes with compact cumulus oophorus were cultivated in different treatments: T1 - TCM 199 with 10% of bovine foetal serum (BFS) (basic medium); T2 – Medium basic and granulosa cells (GC); T3 –Basic medium, GC, 10 IU of e...

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Autor principal: SANTOS, Simone do Socorro Damasceno
Outros Autores: DANTAS, Jedina Kato, MIRANDA, Moysés dos Santos, BIONDI, Flávia Costa, OHASHI, Otávio Mitio
Grau: Artigo
Idioma: por
Publicado em: 2013
Assuntos:
Técnicas de maturação in vitro de oócitos
Bubalinos
Búfalo
Ilha de Marajó - PA
Pará - Estado
Amazônia brasileira
Acesso em linha: http://repositorio.ufpa.br/jspui/handle/2011/3848
id ir-2011-3848
recordtype dspace
spelling ir-2011-38482019-05-23T11:11:44Z Cinética da maturação nuclear in vitro de oócitos bubalinos Nulear maturation kinetcs in vitro of buffalo oocytes SANTOS, Simone do Socorro Damasceno DANTAS, Jedina Kato MIRANDA, Moysés dos Santos BIONDI, Flávia Costa OHASHI, Otávio Mitio Técnicas de maturação in vitro de oócitos Bubalinos Búfalo Ilha de Marajó - PA Pará - Estado Amazônia brasileira To evaluate the kinetics of the in vitro maturation of buffalo oocytes, 1.619 oocytes with compact cumulus oophorus were cultivated in different treatments: T1 - TCM 199 with 10% of bovine foetal serum (BFS) (basic medium); T2 – Medium basic and granulosa cells (GC); T3 –Basic medium, GC, 10 IU of eCG and 10 IU of hCG; T4 – Basic medium , 10 IU of eCG, 10 IU of hCG and 1 µg 17b-estradiol / ml, in times 14 to 17 h, 20 to 22 h, 23 to 25 h, 26 to 28 h, 29 h and 32 h of cultivation in CO2 incubator at 5% and temperature of 38,5ºC. In the treatment 1, it was observed that 81,83%, 69,61%, 65,88%, 60,28%, 64,98% and 67,67% of the oocytes restarted the meiosis; with the treatment 2, rates of oocytes restart of the meiosis were of 76,06%, 72,06%, 69,78%, 68,12%,74,97% and 89,96%; with the treatment 3 rates were of 83,59%, 78,86%, 77,49%, 82,4%, 72,92% and 81,06% and with the treatment 4 the rates were of 95,0%, 91,82%, 93, 17%, 91,65%, 92,09% and 74,99% in the times from 14 to 17 h, 20 to 22 h, 23 to 25 h, 26 to 28 h, 29 h and 32 h of culture, respectively, could be concluded that the supllemented medium provides larger rates of restarted of the meiosis in smaller time, as well as the increase in the time of cultivation it can take to largest rates of degeneration. Com o objetivo de avaliar a cinética da maturação in vitro de oócitos bubalinos, foram cultivados 1.619 oócitos com cumulus oophorus compacto em quatro diferentes tratamentos: T1 - TCM 199 com 10% de Soro Fetal Bovino (SFB) (meio base); T2 - Meio base e células da granulosa (CG); T3 - Meio base, CG, 10 UI de gonadotrofina coriônica equina (eCG) e 10 UI de gonadotrofina coriônica humana (hCG); T4 - Meio base, 10 UI de eCG, 10UI de hCG e 1 µg de 17b-estradiol / ml, em tempos de 14 a 17h, 20 a 22h, 23 a 25h, 26 a 28h, 29h e 32h de cultivo em estufa de CO2 a 5% e temperatura de 38,5ºC. No tratamento 1, observou-se que 81,83%, 69,61%, 65,88%, 60,28%, 64,98% e 67,67% dos ovócitos reiniciaram a meiose. No tratamento 2, as taxas de reinicio da meiose foram de 76,06%, 72,06%, 69,78%, 68,12%,74,97% e 89,96%, no tratamento 3 estes percentuais foram de 83,59%, 78,86%, 77,49%, 82,4%, 72,92% e 81,06% e com o tratamento 4 os índices foram de 95,0%, 91,82%, 93, 17%, 91,65%, 92,09% e 74,99% nos tempos de 14 a 17h, 20 a 22h, 23 a 25h, 26 a 28h, 29h e 32h de cultivo, respectivamente, podendo-se concluir que o meio com maior suplementação proporciona maior taxa de reinício da meiose em menor tempo, assim como o aumento no tempo de cultivo pode levar a maiores índices de degeneração. 2013-05-16T15:15:48Z 2013-05-16T15:15:48Z 2002 Artigo de Periódico SANTOS, Simone do Socorro Damasceno, et al. Cinética da maturação nuclear in vitro de oócitos bubalinos. Brazilian Journal of Veterinary Research and Animal Science, São Paulo, v. 39, n. 5, p. 266-270, 2002. Disponível em: <http://www.scielo.br/pdf/bjvras/v39n5/15839.pdf>. Acesso em: 13 maio 2013. <http://dx.doi.org/10.1590/S1413-95962002000500009>. 1678-4456 1413-9596 http://repositorio.ufpa.br/jspui/handle/2011/3848 por Acesso Aberto application/pdf
institution Repositório Institucional - Universidade Federal do Pará
collection RI-UFPA
language por
topic Técnicas de maturação in vitro de oócitos
Bubalinos
Búfalo
Ilha de Marajó - PA
Pará - Estado
Amazônia brasileira
spellingShingle Técnicas de maturação in vitro de oócitos
Bubalinos
Búfalo
Ilha de Marajó - PA
Pará - Estado
Amazônia brasileira
SANTOS, Simone do Socorro Damasceno
Cinética da maturação nuclear in vitro de oócitos bubalinos
topic_facet Técnicas de maturação in vitro de oócitos
Bubalinos
Búfalo
Ilha de Marajó - PA
Pará - Estado
Amazônia brasileira
description To evaluate the kinetics of the in vitro maturation of buffalo oocytes, 1.619 oocytes with compact cumulus oophorus were cultivated in different treatments: T1 - TCM 199 with 10% of bovine foetal serum (BFS) (basic medium); T2 – Medium basic and granulosa cells (GC); T3 –Basic medium, GC, 10 IU of eCG and 10 IU of hCG; T4 – Basic medium , 10 IU of eCG, 10 IU of hCG and 1 µg 17b-estradiol / ml, in times 14 to 17 h, 20 to 22 h, 23 to 25 h, 26 to 28 h, 29 h and 32 h of cultivation in CO2 incubator at 5% and temperature of 38,5ºC. In the treatment 1, it was observed that 81,83%, 69,61%, 65,88%, 60,28%, 64,98% and 67,67% of the oocytes restarted the meiosis; with the treatment 2, rates of oocytes restart of the meiosis were of 76,06%, 72,06%, 69,78%, 68,12%,74,97% and 89,96%; with the treatment 3 rates were of 83,59%, 78,86%, 77,49%, 82,4%, 72,92% and 81,06% and with the treatment 4 the rates were of 95,0%, 91,82%, 93, 17%, 91,65%, 92,09% and 74,99% in the times from 14 to 17 h, 20 to 22 h, 23 to 25 h, 26 to 28 h, 29 h and 32 h of culture, respectively, could be concluded that the supllemented medium provides larger rates of restarted of the meiosis in smaller time, as well as the increase in the time of cultivation it can take to largest rates of degeneration.
format Artigo
author SANTOS, Simone do Socorro Damasceno
author2 DANTAS, Jedina Kato
MIRANDA, Moysés dos Santos
BIONDI, Flávia Costa
OHASHI, Otávio Mitio
author2Str DANTAS, Jedina Kato
MIRANDA, Moysés dos Santos
BIONDI, Flávia Costa
OHASHI, Otávio Mitio
title Cinética da maturação nuclear in vitro de oócitos bubalinos
title_short Cinética da maturação nuclear in vitro de oócitos bubalinos
title_full Cinética da maturação nuclear in vitro de oócitos bubalinos
title_fullStr Cinética da maturação nuclear in vitro de oócitos bubalinos
title_full_unstemmed Cinética da maturação nuclear in vitro de oócitos bubalinos
title_sort cinética da maturação nuclear in vitro de oócitos bubalinos
publishDate 2013
url http://repositorio.ufpa.br/jspui/handle/2011/3848
_version_ 1787147716119232512
score 11.653393

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