The presente study described the epidemiological and etiological aspects
of acute diarrhea in Juruti, Pará, Brazil. A total of 261 fecal samples were investigated
(diarrheagenic, n = 170 and control, n = 91), from patients attended in Public Health
Units in the period from February to July 2009. Samples were investigated for bacterial,
Rotavírus and parasitic enteropathogens using microscopic examination, immunological
tests (ELISA) and bacterial culture techniques. Two multiplex PCR were designed for
the detection of all categories of diarrheagenic Escherichia coli. A total of 154 samples
were positive (diarrheagenic, n = 118 and control, n = 36), in which 75,4% were
represented for mono infections and 24,6% for mixed infections. Most of cases included
children less than 10 years of age (55,9%), which no significant difference were found
between female and male. The most common enteropathogens in diarrhoeal samples
were E. histolytica/E. dispar (26%), Shigella spp (15,7%), Giardia lamblia (13,3%) and
diarrheagenic E. coli (12,8%), from which Shigella spp (p = 0,0028) were shown to be
associated to the cases of acute diarrhea. The most frequent categories of E. coli in
diarrhoeal cases were ETEC (7,2%) and EAEC (5,9%). The less frequent
enteropathogens, in diarrhoeal samples were Campylobacter jejuni/coli (4,7%),
Rotavírus (2,8%), Salmonella Panama, A. hydrophila and A. sóbria (0,5%). These
results are useful for environmental and epidemiological surveillance of acute diarrhea
disease in Juruti.
|
