Epigenetic alterations in human brain tumors in a Brazilian population
Aberrant methylation of CpG islands located in promoter regions represents one of the major mechanisms for silencing cancer-related genes in tumor cells. We determined the frequency of aberrant CpG island methylation for several tumor-associated genes: DAPK, MGMT, p14ARF, p16INK4a, TP73, RB1 and TIM...
| Autor principal: | ANSELMO, Nilson Praia |
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| Outros Autores: | BELLO, Maria Josefa, GONZALEZ-GOMEZ, Pilar, DIAS, Luis Antonio Araújo, ALMEIDA, José Reinaldo Walter de, SANTOS, Marcelo José dos, HERRANZ, Juan Antonio Rey, CASARTELLI, Cacilda |
| Grau: | Artigo |
| Idioma: | por |
| Publicado em: |
2014
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| Acesso em linha: |
http://repositorio.ufpa.br/jspui/handle/2011/5961 |
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ir-2011-5961 |
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ir-2011-59612023-11-21T15:55:47Z Epigenetic alterations in human brain tumors in a Brazilian population ANSELMO, Nilson Praia BELLO, Maria Josefa GONZALEZ-GOMEZ, Pilar DIAS, Luis Antonio Araújo ALMEIDA, José Reinaldo Walter de SANTOS, Marcelo José dos HERRANZ, Juan Antonio Rey CASARTELLI, Cacilda Epigenética Tumores cerebrais Neoplasias encefálicas Metilação Câncer Meningioma Pará - Estado Amazônia brasileira Aberrant methylation of CpG islands located in promoter regions represents one of the major mechanisms for silencing cancer-related genes in tumor cells. We determined the frequency of aberrant CpG island methylation for several tumor-associated genes: DAPK, MGMT, p14ARF, p16INK4a, TP73, RB1 and TIMP-3 in 55 brain tumors, consisting of 26 neuroepithelial tumors, 6 peripheral nerve tumors, 13 meningeal tumors and 10 metastatic brain tumors. Aberrant methylation of at least one of the seven genes studied was detected in 83.6% of the cases. The frequencies of aberrant methylation were: 40% for p14ARF, 38.2% for MGMT, 30.9% for, p16INK4a, 14.6% for TP73 and for TIMP-3, 12.7% for DAPK and 1.8% for RB1. These data suggest that the hypermethylation observed in the genes p14ARF, MGMT and p16INK4a is a very important event in the formation or progression of brain tumors, since the inactivation of these genes directly interferes with the cell cycle or DNA repair. The altered methylation rate of the other genes has already been reported to be related to tumorigenesis, but the low methylation rate of RB1 found in tumors in our sample is different from that so far reported in the literature, suggesting that perhaps hypermethylation of the promoter is not the main event in the inactivation of this gene. Our results suggest that hypermethylation of the promoter region is a very common event in nervous system tumors. 2014-10-31T14:31:15Z 2014-10-31T14:31:15Z 2006 Artigo de Periódico ANSELMO, Nilson Praia et al. Epigenetic alterations in human brain tumors in a Brazilian population. Genetics and Molecular Biology, São Paulo, v. 29, n. 3, p. 413-422, 2006. Disponível em: <http://www.scielo.br/pdf/gmb/v29n3/30742.pdf>. Acesso em: 07 jul. 2014. <http://dx.doi.org/10.1590/S1415-47572006000300001>. 1415-4757 http://repositorio.ufpa.br/jspui/handle/2011/5961 por Acesso Aberto application/pdf |
| institution |
Repositório Institucional - Universidade Federal do Pará |
| collection |
RI-UFPA |
| language |
por |
| topic |
Epigenética Tumores cerebrais Neoplasias encefálicas Metilação Câncer Meningioma Pará - Estado Amazônia brasileira |
| spellingShingle |
Epigenética Tumores cerebrais Neoplasias encefálicas Metilação Câncer Meningioma Pará - Estado Amazônia brasileira ANSELMO, Nilson Praia Epigenetic alterations in human brain tumors in a Brazilian population |
| topic_facet |
Epigenética Tumores cerebrais Neoplasias encefálicas Metilação Câncer Meningioma Pará - Estado Amazônia brasileira |
| description |
Aberrant methylation of CpG islands located in promoter regions represents one of the major mechanisms for silencing cancer-related genes in tumor cells. We determined the frequency of aberrant CpG island methylation for several tumor-associated genes: DAPK, MGMT, p14ARF, p16INK4a, TP73, RB1 and TIMP-3 in 55 brain tumors, consisting of 26 neuroepithelial tumors, 6 peripheral nerve tumors, 13 meningeal tumors and 10 metastatic brain tumors. Aberrant methylation of at least one of the seven genes studied was detected in 83.6% of the cases. The frequencies of aberrant methylation were: 40% for p14ARF, 38.2% for MGMT, 30.9% for, p16INK4a, 14.6% for TP73 and for TIMP-3, 12.7% for DAPK and 1.8% for RB1. These data suggest that the hypermethylation observed in the genes p14ARF, MGMT and p16INK4a is a very important event in the formation or progression of brain tumors, since the inactivation of these genes directly interferes with the cell cycle or DNA repair. The altered methylation rate of the other genes has already been reported to be related to tumorigenesis, but the low methylation rate of RB1 found in tumors in our sample is different from that so far reported in the literature, suggesting that perhaps hypermethylation of the promoter is not the main event in the inactivation of this gene. Our results suggest that hypermethylation of the promoter region is a very common event in nervous system tumors. |
| format |
Artigo |
| author |
ANSELMO, Nilson Praia |
| author2 |
BELLO, Maria Josefa GONZALEZ-GOMEZ, Pilar DIAS, Luis Antonio Araújo ALMEIDA, José Reinaldo Walter de SANTOS, Marcelo José dos HERRANZ, Juan Antonio Rey CASARTELLI, Cacilda |
| author2Str |
BELLO, Maria Josefa GONZALEZ-GOMEZ, Pilar DIAS, Luis Antonio Araújo ALMEIDA, José Reinaldo Walter de SANTOS, Marcelo José dos HERRANZ, Juan Antonio Rey CASARTELLI, Cacilda |
| title |
Epigenetic alterations in human brain tumors in a Brazilian population |
| title_short |
Epigenetic alterations in human brain tumors in a Brazilian population |
| title_full |
Epigenetic alterations in human brain tumors in a Brazilian population |
| title_fullStr |
Epigenetic alterations in human brain tumors in a Brazilian population |
| title_full_unstemmed |
Epigenetic alterations in human brain tumors in a Brazilian population |
| title_sort |
epigenetic alterations in human brain tumors in a brazilian population |
| publishDate |
2014 |
| url |
http://repositorio.ufpa.br/jspui/handle/2011/5961 |
| _version_ |
1832603642899726336 |
| score |
11.755432 |