Artigo

Brucella abortus detected in cheese from the Amazon region: differentiation of a vaccine strain (B19) from the field strain in the states of Pará, Amapá and Rondônia, Brazil

Brucellosis is an infectious-contagious disease responsible for significant economic losses to the meat and milk supply chain, because it causes reproductive disorders in animals and is a chronic anthropozoonosis. This study was designed to detect the DNA of Brucella spp. in cheese and to differenti...

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Autor principal: BRITO, Jacqueline da Silva
Outros Autores: MORAES, Carina Martins de, SILVA, Cleyzer Lopes, SALES, Gustavo Aguiar, KEID, Lara Borges, MATOS, Paulo Cesar Magalhães, LARA, Ana P.S.S., MORAES, Carla Cristina Guimarães de
Grau: Artigo
Idioma: por
Publicado em: Universidade Federal do Pará 2017
Assuntos:
Brucella abortus
Queijos
Cepa vacinal
Cepa de infecção
Brucelose em animais
Acesso em linha: http://repositorio.ufpa.br/jspui/handle/2011/8926
id ir-2011-8926
recordtype dspace
spelling ir-2011-89262017-12-20T15:30:47Z Brucella abortus detected in cheese from the Amazon region: differentiation of a vaccine strain (B19) from the field strain in the states of Pará, Amapá and Rondônia, Brazil Brucella abortus em queijos na região amazônica: diferenciação em cepa vacinal (B19) ou de infecção a campo nos estados do Pará, Amapá e Rondônia BRITO, Jacqueline da Silva MORAES, Carina Martins de SILVA, Cleyzer Lopes SALES, Gustavo Aguiar KEID, Lara Borges MATOS, Paulo Cesar Magalhães LARA, Ana P.S.S. MORAES, Carla Cristina Guimarães de Brucella abortus Queijos Cepa vacinal Cepa de infecção Brucelose em animais Brucellosis is an infectious-contagious disease responsible for significant economic losses to the meat and milk supply chain, because it causes reproductive disorders in animals and is a chronic anthropozoonosis. This study was designed to detect the DNA of Brucella spp. in cheese and to differentiate between a vaccine strain (B19) and the field strain. Sixty-six samples of different cheeses which are produced and marketed in three states of the Brazilian Amazon region (Amapá [5 samples], Pará [55 samples] and Rondônia [6 samples]) were evaluated. Thirty-nine of these samples were from cheeses made from cow's milk, and 27 were from cheeses made from buffalo milk. Four of the 66 samples were from cheeses produced in milk processing plants regulated by the Federal Inspection Service (Serviço de Inspeção Federal); nine of the samples were from cheeses produced in processing plants regulated by the State Inspection Service (Serviço de Inspeção Estadual); five of the samples were from artisanal cheeses; and the remaining 48 samples were from informally produced cheese. DNA was obtained from the samples following a DNA extraction protocol, and PCR was conducted using primers B4 and B5 to detect Brucella spp. Primers eri1 and eri2 were used to differentiate the field strain from the B19 vaccine strain. The results showed that 21.21% (14/66) of the samples were positive for Brucella spp., of which 21.43% (3/14) were positive for the B. abortus field strain, and 7.14% (1/14) were identified as harboring vaccine strain B19. These results demonstrate that it is possible to identify Brucella spp. in cheese from the Amazon region using the PCR technique and to differentiate the B. abortus field strain from the B19 vaccine strain. A brucelose é uma enfermidade infecto-contagiosa que causa grandes perdas econômicas à cadeia produtiva da carne e do leite, como consequência dos distúrbios reprodutivos nos animais, além de ser uma antropozoonose crônica. O objetivo deste estudo foi detectar DNA de Brucella spp. e fazer a distinção da cepa vacinal (B19) da cepa de infecção de campo. Foram adquiridas 66 amostras de diferentes queijos produzidos e comercializados em três estados pertencentes à Amazônia brasileira: Amapá (05), Pará (55) e Rondônia (06), somando 39 amostras de queijo de vaca e 27 de búfala. Deste total quatro eram produzidas em estabelecimentos com fiscalização de Serviço de Inspeção Federal, nove em estabelecimentos com Serviço de Inspeção Estadual, cinco eram de produção artesanal e as demais 48 amostras eram provenientes de produção informal. O DNA das amostras teste foi obtido por um protocolo de extração e a reação em cadeia pela polimerase foi realizada utilizando os oligoiniciadores B4 e B5 para detectar Brucella spp. e, os oligoiniciadores eri1 e eri2 para diferenciar cepa de infecção a campo da cepa vacinal B19. Os resultados mostraram que 21,21% (14/66) das amostras foram positivas para Brucella spp., destas 21,43% (3/14) foram positivas para B. abortus cepa de campo e 7,14% (1/14) foi identificada como cepa vacinal B19. Concluiu-se que foi possível identificar pela técnica da PCR Brucella spp. em queijos na região amazônica, além de diferenciar as cepas em amostra de B. abortus de infecção a campo ou cepa vacinal B19. 2017-07-28T13:59:18Z 2017-07-28T13:59:18Z 2016-08 Artigo de Periódico BRITO, Jacqueline da Silva et al. Brucella abortus detected in cheese from the Amazon region: differentiation of a vaccine strain (B19) from the field strain in the states of Pará, Amapá and Rondônia, Brazil. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 8, p. 705-710, ago. 2016. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000800705&lng=pt&nrm=iso>. Acesso em: 28 jul. 2017. <http://dx.doi.org/10.1590/S0100-736X2016000800005>. 1678-5150 http://repositorio.ufpa.br/jspui/handle/2011/8926 por Pesquisa Veterinária Brasileira Acesso Aberto application/pdf Universidade Federal do Pará Brasil UFPA
institution Repositório Institucional - Universidade Federal do Pará
collection RI-UFPA
language por
topic Brucella abortus
Queijos
Cepa vacinal
Cepa de infecção
Brucelose em animais
spellingShingle Brucella abortus
Queijos
Cepa vacinal
Cepa de infecção
Brucelose em animais
BRITO, Jacqueline da Silva
Brucella abortus detected in cheese from the Amazon region: differentiation of a vaccine strain (B19) from the field strain in the states of Pará, Amapá and Rondônia, Brazil
topic_facet Brucella abortus
Queijos
Cepa vacinal
Cepa de infecção
Brucelose em animais
description Brucellosis is an infectious-contagious disease responsible for significant economic losses to the meat and milk supply chain, because it causes reproductive disorders in animals and is a chronic anthropozoonosis. This study was designed to detect the DNA of Brucella spp. in cheese and to differentiate between a vaccine strain (B19) and the field strain. Sixty-six samples of different cheeses which are produced and marketed in three states of the Brazilian Amazon region (Amapá [5 samples], Pará [55 samples] and Rondônia [6 samples]) were evaluated. Thirty-nine of these samples were from cheeses made from cow's milk, and 27 were from cheeses made from buffalo milk. Four of the 66 samples were from cheeses produced in milk processing plants regulated by the Federal Inspection Service (Serviço de Inspeção Federal); nine of the samples were from cheeses produced in processing plants regulated by the State Inspection Service (Serviço de Inspeção Estadual); five of the samples were from artisanal cheeses; and the remaining 48 samples were from informally produced cheese. DNA was obtained from the samples following a DNA extraction protocol, and PCR was conducted using primers B4 and B5 to detect Brucella spp. Primers eri1 and eri2 were used to differentiate the field strain from the B19 vaccine strain. The results showed that 21.21% (14/66) of the samples were positive for Brucella spp., of which 21.43% (3/14) were positive for the B. abortus field strain, and 7.14% (1/14) were identified as harboring vaccine strain B19. These results demonstrate that it is possible to identify Brucella spp. in cheese from the Amazon region using the PCR technique and to differentiate the B. abortus field strain from the B19 vaccine strain.
format Artigo
author BRITO, Jacqueline da Silva
author2 MORAES, Carina Martins de
SILVA, Cleyzer Lopes
SALES, Gustavo Aguiar
KEID, Lara Borges
MATOS, Paulo Cesar Magalhães
LARA, Ana P.S.S.
MORAES, Carla Cristina Guimarães de
author2Str MORAES, Carina Martins de
SILVA, Cleyzer Lopes
SALES, Gustavo Aguiar
KEID, Lara Borges
MATOS, Paulo Cesar Magalhães
LARA, Ana P.S.S.
MORAES, Carla Cristina Guimarães de
title Brucella abortus detected in cheese from the Amazon region: differentiation of a vaccine strain (B19) from the field strain in the states of Pará, Amapá and Rondônia, Brazil
title_short Brucella abortus detected in cheese from the Amazon region: differentiation of a vaccine strain (B19) from the field strain in the states of Pará, Amapá and Rondônia, Brazil
title_full Brucella abortus detected in cheese from the Amazon region: differentiation of a vaccine strain (B19) from the field strain in the states of Pará, Amapá and Rondônia, Brazil
title_fullStr Brucella abortus detected in cheese from the Amazon region: differentiation of a vaccine strain (B19) from the field strain in the states of Pará, Amapá and Rondônia, Brazil
title_full_unstemmed Brucella abortus detected in cheese from the Amazon region: differentiation of a vaccine strain (B19) from the field strain in the states of Pará, Amapá and Rondônia, Brazil
title_sort brucella abortus detected in cheese from the amazon region: differentiation of a vaccine strain (b19) from the field strain in the states of pará, amapá and rondônia, brazil
publisher Universidade Federal do Pará
publishDate 2017
url http://repositorio.ufpa.br/jspui/handle/2011/8926
_version_ 1787147671798022144
score 11.675088

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