Molecular analysis reveals the diversity of Hepatozoon species naturally infecting domestic dogs in a northern region of Brazil
This study aimed to optimize molecular methods for detecting DNA of Hepatozoon spp. as well as identify the phylogenetic relationships of Hepatozoon strains naturally infecting domestic dogs in Bel?m, Par?, northern Brazil. Blood samples were collected from 138 dogs, and screened for Hepatozoon spp....
| Autor principal: | Gomes, Laise de Azevedo |
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| Outros Autores: | Moraes, Pablo Henrique Gon?alves, Nascimento, Luciana de C?ssia Silva do, O?Dwyer, Lucia Helena, Nunes, Marcio Roberto Teixeira, Rossi, Adriana dos Reis Ponce, Aguiar, D?lia Cristina Figueira, Gon?alves, Evonnildo Costa |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Elsevier
2017
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br/handle/iec/2494 |
| Resumo: |
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This study aimed to optimize molecular methods for detecting DNA of Hepatozoon spp. as well as identify the phylogenetic relationships of Hepatozoon strains naturally infecting domestic dogs in Bel?m, Par?, northern Brazil. Blood samples were collected from 138 dogs, and screened for Hepatozoon spp. using a new nested PCR assay. Positive samples were subjected to genetic characterization based on amplification and sequencing of approximately 670 bp of the Hepatozoon spp. 18S rRNA. Of the positive dogs, four shared the haplotype Bel?m 01, one dog presented the haplotype Bel?m 02 and two dogs shared the haplotype Bel?m 03. A Bayesian inference indicates that haplotypes Bel?m 01 and Bel?m 02 are phylogenetically related to H. canis, while Bel?m 03 is related to H. americanum. Overall, based on the first molecular evidence of H. americanum in Brazilian domestic dogs, the proposed protocol may improve the epidemiological investigation of canine hepatozoonosis. |