Molecular analysis reveals the diversity of Hepatozoon species naturally infecting domestic dogs in a northern region of Brazil

Gomes, Laise de Azevedo; Moraes, Pablo Henrique Gon?alves; Nascimento, Luciana de C?ssia Silva do; O?Dwyer, Lucia Helena; Nunes, Marcio Roberto Teixeira; Rossi, Adriana dos Reis Ponce; Aguiar, D?lia Cristina Figueira; Gon?alves, Evonnildo Costa

Artigo

Molecular analysis reveals the diversity of Hepatozoon species naturally infecting domestic dogs in a northern region of Brazil

This study aimed to optimize molecular methods for detecting DNA of Hepatozoon spp. as well as identify the phylogenetic relationships of Hepatozoon strains naturally infecting domestic dogs in Bel?m, Par?, northern Brazil. Blood samples were collected from 138 dogs, and screened for Hepatozoon spp....

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Autor principal:	Gomes, Laise de Azevedo
Outros Autores:	Moraes, Pablo Henrique Gon?alves, Nascimento, Luciana de C?ssia Silva do, O?Dwyer, Lucia Helena, Nunes, Marcio Roberto Teixeira, Rossi, Adriana dos Reis Ponce, Aguiar, D?lia Cristina Figueira, Gon?alves, Evonnildo Costa
Grau:	Artigo
Idioma:	eng
Publicado em:	Elsevier 2017
Assuntos:	An?lise Molecular Hepatozoon spp Hepatozoons C?es Dom?sticos Hepatozoonose Canina. Hepatozoon americanum Hepatozoon canis Regi?o Norte (BR)
Acesso em linha:	http://patua.iec.gov.br/handle/iec/2494

id	ir-iec-2494
recordtype	dspace
spelling	ir-iec-24942018-11-14T12:40:53Z Molecular analysis reveals the diversity of Hepatozoon species naturally infecting domestic dogs in a northern region of Brazil Gomes, Laise de Azevedo Moraes, Pablo Henrique Gon?alves Nascimento, Luciana de C?ssia Silva do O?Dwyer, Lucia Helena Nunes, Marcio Roberto Teixeira Rossi, Adriana dos Reis Ponce Aguiar, D?lia Cristina Figueira Gon?alves, Evonnildo Costa An?lise Molecular Hepatozoon spp Hepatozoons C?es Dom?sticos Hepatozoonose Canina. Hepatozoon americanum Hepatozoon canis Regi?o Norte (BR) This study aimed to optimize molecular methods for detecting DNA of Hepatozoon spp. as well as identify the phylogenetic relationships of Hepatozoon strains naturally infecting domestic dogs in Bel?m, Par?, northern Brazil. Blood samples were collected from 138 dogs, and screened for Hepatozoon spp. using a new nested PCR assay. Positive samples were subjected to genetic characterization based on amplification and sequencing of approximately 670 bp of the Hepatozoon spp. 18S rRNA. Of the positive dogs, four shared the haplotype Bel?m 01, one dog presented the haplotype Bel?m 02 and two dogs shared the haplotype Bel?m 03. A Bayesian inference indicates that haplotypes Bel?m 01 and Bel?m 02 are phylogenetically related to H. canis, while Bel?m 03 is related to H. americanum. Overall, based on the first molecular evidence of H. americanum in Brazilian domestic dogs, the proposed protocol may improve the epidemiological investigation of canine hepatozoonosis. Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) - (grant 311686/2015-0); Conselho de Aperfei?oamento de Pessoal cient?fico e Tecnol?gico (CNPq) - (grant 311686/2015-0) 2017-02-08T19:42:26Z 2017-02-08T19:42:26Z 2016 Artigo GOMES, Laise de Azevedo et al. Molecular analysis reveals the diversity of Hepatozoon species naturally infecting domestic dogs in a northern region of Brazil. Ticks and Tick-borne Diseases, v. 7, n. 6, p. 1061-1066, Oct. 2016. 1877-9603 http://patua.iec.gov.br/handle/iec/2494 10.1016/j.ttbdis.2016.09.008 eng Acesso Embargado application/pdf Elsevier
institution	Instituto Evandro Chagas (IEC)
collection	PATUA
language	eng
topic	An?lise Molecular Hepatozoon spp Hepatozoons C?es Dom?sticos Hepatozoonose Canina. Hepatozoon americanum Hepatozoon canis Regi?o Norte (BR)
spellingShingle	An?lise Molecular Hepatozoon spp Hepatozoons C?es Dom?sticos Hepatozoonose Canina. Hepatozoon americanum Hepatozoon canis Regi?o Norte (BR) Gomes, Laise de Azevedo Molecular analysis reveals the diversity of Hepatozoon species naturally infecting domestic dogs in a northern region of Brazil
topic_facet	An?lise Molecular Hepatozoon spp Hepatozoons C?es Dom?sticos Hepatozoonose Canina. Hepatozoon americanum Hepatozoon canis Regi?o Norte (BR)
description	This study aimed to optimize molecular methods for detecting DNA of Hepatozoon spp. as well as identify the phylogenetic relationships of Hepatozoon strains naturally infecting domestic dogs in Bel?m, Par?, northern Brazil. Blood samples were collected from 138 dogs, and screened for Hepatozoon spp. using a new nested PCR assay. Positive samples were subjected to genetic characterization based on amplification and sequencing of approximately 670 bp of the Hepatozoon spp. 18S rRNA. Of the positive dogs, four shared the haplotype Bel?m 01, one dog presented the haplotype Bel?m 02 and two dogs shared the haplotype Bel?m 03. A Bayesian inference indicates that haplotypes Bel?m 01 and Bel?m 02 are phylogenetically related to H. canis, while Bel?m 03 is related to H. americanum. Overall, based on the first molecular evidence of H. americanum in Brazilian domestic dogs, the proposed protocol may improve the epidemiological investigation of canine hepatozoonosis.
format	Artigo
author	Gomes, Laise de Azevedo
author2	Moraes, Pablo Henrique Gon?alves Nascimento, Luciana de C?ssia Silva do O?Dwyer, Lucia Helena Nunes, Marcio Roberto Teixeira Rossi, Adriana dos Reis Ponce Aguiar, D?lia Cristina Figueira Gon?alves, Evonnildo Costa
author2Str	Moraes, Pablo Henrique Gon?alves Nascimento, Luciana de C?ssia Silva do O?Dwyer, Lucia Helena Nunes, Marcio Roberto Teixeira Rossi, Adriana dos Reis Ponce Aguiar, D?lia Cristina Figueira Gon?alves, Evonnildo Costa
title	Molecular analysis reveals the diversity of Hepatozoon species naturally infecting domestic dogs in a northern region of Brazil
title_short	Molecular analysis reveals the diversity of Hepatozoon species naturally infecting domestic dogs in a northern region of Brazil
title_full	Molecular analysis reveals the diversity of Hepatozoon species naturally infecting domestic dogs in a northern region of Brazil
title_fullStr	Molecular analysis reveals the diversity of Hepatozoon species naturally infecting domestic dogs in a northern region of Brazil
title_full_unstemmed	Molecular analysis reveals the diversity of Hepatozoon species naturally infecting domestic dogs in a northern region of Brazil
title_sort	molecular analysis reveals the diversity of hepatozoon species naturally infecting domestic dogs in a northern region of brazil
publisher	Elsevier
publishDate	2017
url	http://patua.iec.gov.br/handle/iec/2494
_version_	1717584471233921024
score	11.755432

Molecular analysis reveals the diversity of Hepatozoon species naturally infecting domestic dogs in a northern region of Brazil

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