Norovirus genogroups I and II in environmental water samples from Bel?m city, Northern Brazil

Teixeira, Dielle Monteiro; Spada, Paula Katharine de Pontes; Morais, Lena L?llian Canto de S?; Fumian, Tulio Machado; Lima, Ian Carlos Gomes de; Oliveira, Darleise de Souza; Bandeira, Renato da Silva; Gurj?o, Tereza Cristina Monteiro; Sousa, Ma?sa Silva de; Mascarenhas, Joana D'Arc Pereira; Gabbay, Yvone Benchimol

Artigo

Norovirus genogroups I and II in environmental water samples from Bel?m city, Northern Brazil

This study investigated the presence of norovirus (NoV) GI and GII in environmental samples from the northern region of Brazil. Water samples were collected monthly (November 2008/October 2010) from different sources and sewage and concentrated by the adsorption-elution method. The NoV investigation...

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Autor principal:	Teixeira, Dielle Monteiro
Outros Autores:	Spada, Paula Katharine de Pontes, Morais, Lena L?llian Canto de S?, Fumian, Tulio Machado, Lima, Ian Carlos Gomes de, Oliveira, Darleise de Souza, Bandeira, Renato da Silva, Gurj?o, Tereza Cristina Monteiro, Sousa, Ma?sa Silva de, Mascarenhas, Joana D'Arc Pereira, Gabbay, Yvone Benchimol
Grau:	Artigo
Idioma:	eng
Publicado em:	IWA Publishing 2017
Assuntos:	Norovirus / isolamento & purifica??o Norovirus / gen?tica ?gua Doce / microbiologia Esgotos / microbiologia Filogenia Gen?tipo An?lise de Sequ?ncia de RNA Fases de Leitura Aberta Monitoramento Ambiental / m?todos Rea??o em Cadeia da Polimerase em Tempo Real / m?todos Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos
Acesso em linha:	http://patua.iec.gov.br//handle/iec/2865

id	ir-iec-2865
recordtype	dspace
spelling	ir-iec-28652018-11-14T14:03:58Z Norovirus genogroups I and II in environmental water samples from Bel?m city, Northern Brazil Teixeira, Dielle Monteiro Spada, Paula Katharine de Pontes Morais, Lena L?llian Canto de S? Fumian, Tulio Machado Lima, Ian Carlos Gomes de Oliveira, Darleise de Souza Bandeira, Renato da Silva Gurj?o, Tereza Cristina Monteiro Sousa, Ma?sa Silva de Mascarenhas, Joana D'Arc Pereira Gabbay, Yvone Benchimol Norovirus / isolamento & purifica??o Norovirus / gen?tica ?gua Doce / microbiologia Esgotos / microbiologia Filogenia Gen?tipo An?lise de Sequ?ncia de RNA Fases de Leitura Aberta Monitoramento Ambiental / m?todos Rea??o em Cadeia da Polimerase em Tempo Real / m?todos Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos This study investigated the presence of norovirus (NoV) GI and GII in environmental samples from the northern region of Brazil. Water samples were collected monthly (November 2008/October 2010) from different sources and sewage and concentrated by the adsorption-elution method. The NoV investigation used molecular methods followed by sequencing reactions. The general positivity for NoV was 33.9% (57/168). Considering the results obtained only in the semi-nested RT-PCR (reverse transcription polymerase chain reaction) and only in the TaqMan? real-time PCR, the rates were 26.8% (45/168) and 27.4% (46/168), respectively, being for NoV GI 22.2% (10/45) and 19.6% (9/46); for GII 17.8% (8/45) and 15.2% (7/46); and for GI + GII 60% (27/45) and 65.2% (30/46), respectively. Different GI (GI.1, GI.4, GI.7 and GI.8) and GII (GII.4, GII.6, GII.9, GII.12 and GII.14) genotypes were detected. These results demonstrated the NoV was disseminated in the waters of Bel?m city due to a lack of sanitation that allowed the discharge of contaminated effluents into these aquatic ecosystems. 2017-11-21T19:49:20Z 2017-11-21T19:49:20Z 2017 Artigo TEIXEIRA, Dielle Monteiro et al. Norovirus genogroups I and II in environmental water samples from Bel?m city, Northern Brazil. Journal of Water and Health, v. 15, n. 1, p. 163-174, Feb. 2017. 1477-8920 http://patua.iec.gov.br//handle/iec/2865 10.2166/wh.2016.275. eng Acesso Aberto application/pdf 2008 2010 IWA Publishing
institution	Instituto Evandro Chagas (IEC)
collection	PATUA
language	eng
topic	Norovirus / isolamento & purifica??o Norovirus / gen?tica ?gua Doce / microbiologia Esgotos / microbiologia Filogenia Gen?tipo An?lise de Sequ?ncia de RNA Fases de Leitura Aberta Monitoramento Ambiental / m?todos Rea??o em Cadeia da Polimerase em Tempo Real / m?todos Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos
spellingShingle	Norovirus / isolamento & purifica??o Norovirus / gen?tica ?gua Doce / microbiologia Esgotos / microbiologia Filogenia Gen?tipo An?lise de Sequ?ncia de RNA Fases de Leitura Aberta Monitoramento Ambiental / m?todos Rea??o em Cadeia da Polimerase em Tempo Real / m?todos Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos Teixeira, Dielle Monteiro Norovirus genogroups I and II in environmental water samples from Bel?m city, Northern Brazil
topic_facet	Norovirus / isolamento & purifica??o Norovirus / gen?tica ?gua Doce / microbiologia Esgotos / microbiologia Filogenia Gen?tipo An?lise de Sequ?ncia de RNA Fases de Leitura Aberta Monitoramento Ambiental / m?todos Rea??o em Cadeia da Polimerase em Tempo Real / m?todos Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos
description	This study investigated the presence of norovirus (NoV) GI and GII in environmental samples from the northern region of Brazil. Water samples were collected monthly (November 2008/October 2010) from different sources and sewage and concentrated by the adsorption-elution method. The NoV investigation used molecular methods followed by sequencing reactions. The general positivity for NoV was 33.9% (57/168). Considering the results obtained only in the semi-nested RT-PCR (reverse transcription polymerase chain reaction) and only in the TaqMan? real-time PCR, the rates were 26.8% (45/168) and 27.4% (46/168), respectively, being for NoV GI 22.2% (10/45) and 19.6% (9/46); for GII 17.8% (8/45) and 15.2% (7/46); and for GI + GII 60% (27/45) and 65.2% (30/46), respectively. Different GI (GI.1, GI.4, GI.7 and GI.8) and GII (GII.4, GII.6, GII.9, GII.12 and GII.14) genotypes were detected. These results demonstrated the NoV was disseminated in the waters of Bel?m city due to a lack of sanitation that allowed the discharge of contaminated effluents into these aquatic ecosystems.
format	Artigo
author	Teixeira, Dielle Monteiro
author2	Spada, Paula Katharine de Pontes Morais, Lena L?llian Canto de S? Fumian, Tulio Machado Lima, Ian Carlos Gomes de Oliveira, Darleise de Souza Bandeira, Renato da Silva Gurj?o, Tereza Cristina Monteiro Sousa, Ma?sa Silva de Mascarenhas, Joana D'Arc Pereira Gabbay, Yvone Benchimol
author2Str	Spada, Paula Katharine de Pontes Morais, Lena L?llian Canto de S? Fumian, Tulio Machado Lima, Ian Carlos Gomes de Oliveira, Darleise de Souza Bandeira, Renato da Silva Gurj?o, Tereza Cristina Monteiro Sousa, Ma?sa Silva de Mascarenhas, Joana D'Arc Pereira Gabbay, Yvone Benchimol
title	Norovirus genogroups I and II in environmental water samples from Bel?m city, Northern Brazil
title_short	Norovirus genogroups I and II in environmental water samples from Bel?m city, Northern Brazil
title_full	Norovirus genogroups I and II in environmental water samples from Bel?m city, Northern Brazil
title_fullStr	Norovirus genogroups I and II in environmental water samples from Bel?m city, Northern Brazil
title_full_unstemmed	Norovirus genogroups I and II in environmental water samples from Bel?m city, Northern Brazil
title_sort	norovirus genogroups i and ii in environmental water samples from bel?m city, northern brazil
publisher	IWA Publishing
publishDate	2017
url	http://patua.iec.gov.br//handle/iec/2865
_version_	1717584502216196096
score	11.755432

Norovirus genogroups I and II in environmental water samples from Bel?m city, Northern Brazil

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