Evaluation of the commercial kit SIRE Nitratase for detecting resistant Mycobacterium tuberculosis in Brazil
Introduction: This study aimed to evaluate a new commercial kit, Kit SIRE Nitratase-PlastLabor, for testing the drug susceptibility of clinical Mycobacterium tuberculosis isolates. Methods: The accuracy of the Kit SIRE Nitratase was evaluated by examining the susceptibility (streptomycin, isoniazi...
| Autor principal: | Miranda, Silvana Spindola de |
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| Outros Autores: | Almeida, Isabela Neves de, Lopes, Maria Luiza, Figueiredo, Jamilly dos Reis de, Figueredo, Lida Jouca de Assis, Kritski, Afr?nio Lineu, Carvalho, W?nia da Silva, Mansur, Maria de F?tima Filardi Oliveira |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Sociedade Brasileira de Medicina Tropical
2017
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/2870 |
| Resumo: |
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Introduction: This study aimed to evaluate a new commercial kit, Kit SIRE Nitratase-PlastLabor, for testing the drug susceptibility
of clinical Mycobacterium tuberculosis isolates. Methods: The accuracy of the Kit SIRE Nitratase was evaluated by examining
the susceptibility (streptomycin, isoniazid, rifampicin, and ethambutol) of 40 M. tuberculosis isolates, using the proportion method
with Lowenstein-Jensen medium or the BACTEC MGIT 960 system. Results: The detection accuracy for streptomycin, isoniazid,
rifampicin, and ethambutol was 95%, 97.5%, 100%, and 80%, respectively. Conclusions: The exceptional accuracy demonstrated
by Kit SIRE Nitratase for isoniazid and rifampicin makes the kit an attractive option for screening M. tuberculosis strain resistance. |