Artigo

Detection of drug resistant Mycobacterium Tuberculosis strains using kit SIRE Nitratase?: a multicenter study

Background: The Commercial Kit SIRE Nitratase? PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy,...

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Autor principal: Miranda, Silvana Spindola de
Outros Autores: Almeida, Isabela Neves de, Mansur, Maria de F?tima Filardi Oliveira, Figueredo, Lida Jouca de Assis, Carvalho, W?nia da Silva, Hadaad, Jo?o Paulo Amaral, Diniz, Jaciara de Lourdes do Carmo Guimar?es, Groll, Andrea Von, Silva, Pedro Almeida da, Lopes, Maria Luiza, Santos, Marcelo Cordeiro dos, Brito, Alexandra, Mello, Fernanda Carvalho de Queiroz, Malaquias, Thiago da Silva Santos, Croda, Julio, Pinhata, Juliana Maira Watanabe, Oliveira, Ros?ngela Siqueira de, Chimara, Erica, Rossetti, Maria L?cia, Halon, Maria Laura, Louren?o, Maria Cristina, Medeiros, Reginalda Ferreira de Melo, Montes, F?tima Cristina Onofre Fandinho, Machado, Diana, Vivieiros, Miguel, Kritski, Afr?nio Lineu
Grau: Artigo
Idioma: eng
Publicado em: Instituto de Tecnologia do Paran? 2020
Assuntos:
Tuberculose / diagn?stico
Resist?ncia a M?ltiplos Medicamentos
Mycobacterium / efeitos dos f?rmacos
Sensibilidade e Especificidade
Nitrato Redutase / farmacologia
Estudo Multic?ntrico
Acesso em linha: http://patua.iec.gov.br//handle/iec/4147
Resumo:
Background: The Commercial Kit SIRE Nitratase? PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.

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