Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2)
BACKGROUND: The genetic diversity of malaria antigens often results in allele variant-specific immunity, imposing a great challenge to vaccine development. Rhoptry Neck Protein 2 (PvRON2) is a blood-stage antigen that plays a key role during the erythrocyte invasion of Plasmodium vivax. This study i...
| Autor principal: | Bittencourt, Najara C |
|---|---|
| Outros Autores: | Leite, Juliana A, Silva, Ana Beatriz I. E, Pimenta, Tamirys S, Silva Filho, Jo?o Luiz, Cassiano, Gustavo C, Lopes, Stefanie C. P, Santos, Jo?o C. K. dos, Bourgard, Catarina, Nakaya, Helder I, Ventura, Ana Maria Revor?do da Silva, Lacerda, Marcus V. G, Ferreira, Marcelo U, Machado, Ricardo L. D, Albrecht, Letusa, Costa, Fabio T. M |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
BMC
2018
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/3522 |
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ir-iec-3522 |
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ir-iec-35222018-11-12T17:34:29Z Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2) Bittencourt, Najara C Leite, Juliana A Silva, Ana Beatriz I. E Pimenta, Tamirys S Silva Filho, Jo?o Luiz Cassiano, Gustavo C Lopes, Stefanie C. P Santos, Jo?o C. K. dos Bourgard, Catarina Nakaya, Helder I Ventura, Ana Maria Revor?do da Silva Lacerda, Marcus V. G Ferreira, Marcelo U Machado, Ricardo L. D Albrecht, Letusa Costa, Fabio T. M Mal?ria vivax / parasitologia Plasmodium vivax / parasitologia Rea??es Ant?geno-Anticorpo Ant?genos Varia??o Gen?tica / imunologia Vacinas / imunologia BACKGROUND: The genetic diversity of malaria antigens often results in allele variant-specific immunity, imposing a great challenge to vaccine development. Rhoptry Neck Protein 2 (PvRON2) is a blood-stage antigen that plays a key role during the erythrocyte invasion of Plasmodium vivax. This study investigates the genetic diversity of PvRON2 and the naturally acquired immune response to P. vivax isolates. RESULTS: Here, the genetic diversity of PvRON21828-2080 and the naturally acquired humoral immune response against PvRON21828-2080 in infected and non-infected individuals from a vivax malaria endemic area in Brazil was reported. The diversity analysis of PvRON21828-2080 revealed that the protein is conserved in isolates in Brazil and worldwide. A total of 18 (19%) patients had IgG antibodies to PvRON21828-2080. Additionally, the analysis of the antibody response in individuals who were not acutely infected with malaria, but had been infected with malaria in the past indicated that 32 patients (33%) exhibited an IgG immune response against PvRON2. CONCLUSIONS: PvRON2 was conserved among the studied isolates. The presence of naturally acquired antibodies to this protein in the absence of the disease suggests that PvRON2 induces a long-term antibody response. These results indicate that PvRON2 is a potential malaria vaccine candidate. This work was supported by Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo (FAPESP) Grant 2012/16525-2, and fellowships grants 2015/02808-0 (to NB), 2013/25807-4 (to JAL), 2016/12855-9 (to JLS-F), 2015/20774-6 (to GCC), 2013/20509-5 (to CB). Evandro Chagas core grant (to RLDM). MUF, MVGL and FTMC are Conselho Nacional do Desenvolvimento Cient?fico e Tecnol?gico (CNPq) research fellows. 2018-11-12T17:05:57Z 2018-11-12T17:05:57Z 2018 Artigo BITTENCOURT, Najara C. et al. Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2). Malaria Journal, v. 17, n. 1, p. 401, Oct. 2018. 1475-2875 http://patua.iec.gov.br//handle/iec/3522 10.1186/s12936-018-2543-7. eng Acesso Aberto application/pdf BMC |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Mal?ria vivax / parasitologia Plasmodium vivax / parasitologia Rea??es Ant?geno-Anticorpo Ant?genos Varia??o Gen?tica / imunologia Vacinas / imunologia |
| spellingShingle |
Mal?ria vivax / parasitologia Plasmodium vivax / parasitologia Rea??es Ant?geno-Anticorpo Ant?genos Varia??o Gen?tica / imunologia Vacinas / imunologia Bittencourt, Najara C Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2) |
| topic_facet |
Mal?ria vivax / parasitologia Plasmodium vivax / parasitologia Rea??es Ant?geno-Anticorpo Ant?genos Varia??o Gen?tica / imunologia Vacinas / imunologia |
| description |
BACKGROUND: The genetic diversity of malaria antigens often results in allele variant-specific immunity, imposing a great challenge to vaccine development. Rhoptry Neck Protein 2 (PvRON2) is a blood-stage antigen that plays a key role during the erythrocyte invasion of Plasmodium vivax. This study investigates the genetic diversity of PvRON2 and the naturally acquired immune response to P. vivax isolates. RESULTS: Here, the genetic diversity of PvRON21828-2080 and the naturally acquired humoral immune response against PvRON21828-2080 in infected and non-infected individuals from a vivax malaria endemic area in Brazil was reported. The diversity analysis of PvRON21828-2080 revealed that the protein is conserved in isolates in Brazil and worldwide. A total of 18 (19%) patients had IgG antibodies to PvRON21828-2080. Additionally, the analysis of the antibody response in individuals who were not acutely infected with malaria, but had been infected with malaria in the past indicated that 32 patients (33%) exhibited an IgG immune response against PvRON2. CONCLUSIONS: PvRON2 was conserved among the studied isolates. The presence of naturally acquired antibodies to this protein in the absence of the disease suggests that PvRON2 induces a long-term antibody response. These results indicate that PvRON2 is a potential malaria vaccine candidate. |
| format |
Artigo |
| author |
Bittencourt, Najara C |
| author2 |
Leite, Juliana A Silva, Ana Beatriz I. E Pimenta, Tamirys S Silva Filho, Jo?o Luiz Cassiano, Gustavo C Lopes, Stefanie C. P Santos, Jo?o C. K. dos Bourgard, Catarina Nakaya, Helder I Ventura, Ana Maria Revor?do da Silva Lacerda, Marcus V. G Ferreira, Marcelo U Machado, Ricardo L. D Albrecht, Letusa Costa, Fabio T. M |
| author2Str |
Leite, Juliana A Silva, Ana Beatriz I. E Pimenta, Tamirys S Silva Filho, Jo?o Luiz Cassiano, Gustavo C Lopes, Stefanie C. P Santos, Jo?o C. K. dos Bourgard, Catarina Nakaya, Helder I Ventura, Ana Maria Revor?do da Silva Lacerda, Marcus V. G Ferreira, Marcelo U Machado, Ricardo L. D Albrecht, Letusa Costa, Fabio T. M |
| title |
Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2) |
| title_short |
Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2) |
| title_full |
Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2) |
| title_fullStr |
Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2) |
| title_full_unstemmed |
Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2) |
| title_sort |
genetic sequence characterization and naturally acquired immune response to plasmodium vivax rhoptry neck protein 2 (pvron2) |
| publisher |
BMC |
| publishDate |
2018 |
| url |
http://patua.iec.gov.br//handle/iec/3522 |
| _version_ |
1717584329160261632 |
| score |
11.755432 |