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Artigo
Genomic analysis of rotavirus species A isolated from swine, Amazon Region, Brazil
This present study has aimed to provide a molecular characterization of rotavirus species A (RVA) and to determine the frequency of RVC and RVH in fecal specimens from nonvaccinated piglets, across 5 farms in the metropolitan mesoregion of Bel?m, within the Amazon region, Brazil. In the previous s...
Autor principal: | Neves, Mayara A. O |
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Outros Autores: | Camargo, Daniel S. de, Ara?jo, Kathleen V. S. de, Lobo, Patr?cia S, Bandeira, Renato da S, Soares, Luana da Silva, Mascarenhas, Joana D'Arc Pereira |
Grau: | Artigo |
Idioma: | eng |
Publicado em: |
Krishna Institute of Science
2019
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Assuntos: | |
Acesso em linha: |
http://patua.iec.gov.br//handle/iec/3638 |
Resumo: |
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This present study has aimed to provide a molecular characterization of rotavirus species A
(RVA) and to determine the frequency of RVC and RVH in fecal specimens from nonvaccinated piglets, across 5 farms in the metropolitan mesoregion of Bel?m, within the
Amazon region, Brazil. In the previous study, porcine RVA was found in 9.9% (17/172)
during the years 2008-2009. Using RT-PCR, Sanger nucleotide sequencing, and
phylogenetic analyses, the entire genomes of 17 Brazilian porcine RVA strains were
analyzed. The results indicated that all strains shared the same constellation, with the
exception of the VP3 and NSP3 genes, which were M2 and T7 genotypes, respectively.
The VP1, VP2, NSP2, NSP4 and NSP5 coding segments clustered into the Wa-like
constellation; genotype 1, and VP7, VP4, VP6 and NSP1 genes were typically of porcine
origin. RVA genes infecting pigs presented a high degree of nucleotide similarity to strains
from other hosts, including humans. Little is known about the zoonotic potential of
rotaviruses in Brazil, but the results of this study show dynamic interactions between
human and porcine rotavirus strains and constitute an increase in knowledge on the
diversity of RVA strains, in animal reservoirs circulating in the Amazon region. |