Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants
The immunogenicity and efficacy of a hybrid recombinant protein derived from the N-terminal end of the glutamate-rich protein (GLURP) and the C-terminal portion of the merozoite surface protein 3 (MSP3) of Plasmodium falciparum was evaluated in Saimiri sciureus monkeys. The GLURP/MSP3 hybrid protei...
| Autor principal: | Carvalho, Leonardo J. M. de |
|---|---|
| Outros Autores: | Alves, Francisco A., Bianco Jr, Casare, Oliveira, Salma Gomes, Zanini, Graziela Maria, Soe, Soe, Druilhe, Pierre, Theisen, Michael, Muniz, Jos? Augusto Pereira Carneiro, Daniel-Ribeiro, Cl?udio Tadeu |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
American Society for Microbiology
2019
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/3737 |
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ir-iec-3737 |
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dspace |
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ir-iec-37372019-06-04T16:51:47Z Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants Carvalho, Leonardo J. M. de Alves, Francisco A. Bianco Jr, Casare Oliveira, Salma Gomes Zanini, Graziela Maria Soe, Soe Druilhe, Pierre Theisen, Michael Muniz, Jos? Augusto Pereira Carneiro Daniel-Ribeiro, Cl?udio Tadeu Saimiri / imunologia Plasmodium falciparum / imunologia Imuniza??o / veterin?ria Mal?ria / preven??o & controle Vacinas Antimal?ricas / farmacologia The immunogenicity and efficacy of a hybrid recombinant protein derived from the N-terminal end of the glutamate-rich protein (GLURP) and the C-terminal portion of the merozoite surface protein 3 (MSP3) of Plasmodium falciparum was evaluated in Saimiri sciureus monkeys. The GLURP/MSP3 hybrid protein, expressed in Lactococcus lactis, was administered in association with alum, Montanide ISA720, or complete or incomplete Freund adjuvant (CFA/IFA) in groups of five animals each. The three formulations were shown to be immunogenic, but the one with alum was shown to be weak compared to the other two, particularly CFA/IFA, which provided very high antibody titers (enzyme-linked immunosorbent assay titers of >3,000,000 and immunofluorescence antibody test titers of 6,400). After a challenge infection with P. falciparum FUP strain, all five monkeys from the GLURP/MSP3- alum group showed a rapid increase in parasitemia, reaching 10% and were treated early. The two monkeys with the highest antibody titers in group GLURP/MSP3-Montanide ISA720 had a delay in the course of parasitemia and were treated late due to a low hematocrit. In the GLURP/MSP3-CFA/IFA group, parasitemia remained below this threshold in four of the five animals and, after it reached a peak, parasitemia started to decrease and monkeys were treated late. When all animals were grouped according to the outcome, a statistically significant association between high antibody titers and partial protection was observed. The challenge infection boosted the antibody titers, and the importance of this event for vaccine efficacy in areas where this parasite is endemic is discussed. In conclusion, these data suggest that GLURP and MSP3 can induce protection against malaria infection if antibodies are induced at properly high titers. European Malaria Vaccine Initiative program of the Commission of the European Communities, the Brazilian National Research Council (CNPq), the Programa de Desenvolvimento Tecnolo?gico em Insumos para Sau?de (PDTIS/Fiocruz), and the Instituto Oswaldo Cruz/Fiocruz and with resources from the National Primate Center and Instituto Evandro Chagas/SVS 2019-06-04T16:39:01Z 2019-06-04T16:39:01Z 2005 Artigo CARVALHO, Leonardo J. M. de et al. Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants. Clinical and Diagnostic Laboratory Immunology, v. 12, n. 2, p. 242-248, Feb. 2005. 1071-412x http://patua.iec.gov.br//handle/iec/3737 10.1128/CDLI.12.2.242?248.2005 eng Acesso Aberto application/pdf American Society for Microbiology |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Saimiri / imunologia Plasmodium falciparum / imunologia Imuniza??o / veterin?ria Mal?ria / preven??o & controle Vacinas Antimal?ricas / farmacologia |
| spellingShingle |
Saimiri / imunologia Plasmodium falciparum / imunologia Imuniza??o / veterin?ria Mal?ria / preven??o & controle Vacinas Antimal?ricas / farmacologia Carvalho, Leonardo J. M. de Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants |
| topic_facet |
Saimiri / imunologia Plasmodium falciparum / imunologia Imuniza??o / veterin?ria Mal?ria / preven??o & controle Vacinas Antimal?ricas / farmacologia |
| description |
The immunogenicity and efficacy of a hybrid recombinant protein derived from the N-terminal end of the
glutamate-rich protein (GLURP) and the C-terminal portion of the merozoite surface protein 3 (MSP3) of Plasmodium falciparum was evaluated in Saimiri sciureus monkeys. The GLURP/MSP3 hybrid protein, expressed in
Lactococcus lactis, was administered in association with alum, Montanide ISA720, or complete or incomplete Freund
adjuvant (CFA/IFA) in groups of five animals each. The three formulations were shown to be immunogenic, but the
one with alum was shown to be weak compared to the other two, particularly CFA/IFA, which provided very high
antibody titers (enzyme-linked immunosorbent assay titers of >3,000,000 and immunofluorescence antibody test
titers of 6,400). After a challenge infection with P. falciparum FUP strain, all five monkeys from the GLURP/MSP3-
alum group showed a rapid increase in parasitemia, reaching 10% and were treated early. The two monkeys with
the highest antibody titers in group GLURP/MSP3-Montanide ISA720 had a delay in the course of parasitemia and
were treated late due to a low hematocrit. In the GLURP/MSP3-CFA/IFA group, parasitemia remained below this
threshold in four of the five animals and, after it reached a peak, parasitemia started to decrease and monkeys were
treated late. When all animals were grouped according to the outcome, a statistically significant association between
high antibody titers and partial protection was observed. The challenge infection boosted the antibody titers, and
the importance of this event for vaccine efficacy in areas where this parasite is endemic is discussed. In conclusion,
these data suggest that GLURP and MSP3 can induce protection against malaria infection if antibodies are induced
at properly high titers. |
| format |
Artigo |
| author |
Carvalho, Leonardo J. M. de |
| author2 |
Alves, Francisco A. Bianco Jr, Casare Oliveira, Salma Gomes Zanini, Graziela Maria Soe, Soe Druilhe, Pierre Theisen, Michael Muniz, Jos? Augusto Pereira Carneiro Daniel-Ribeiro, Cl?udio Tadeu |
| author2Str |
Alves, Francisco A. Bianco Jr, Casare Oliveira, Salma Gomes Zanini, Graziela Maria Soe, Soe Druilhe, Pierre Theisen, Michael Muniz, Jos? Augusto Pereira Carneiro Daniel-Ribeiro, Cl?udio Tadeu |
| title |
Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants |
| title_short |
Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants |
| title_full |
Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants |
| title_fullStr |
Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants |
| title_full_unstemmed |
Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants |
| title_sort |
immunization of saimiri sciureus monkeys with a recombinant hybrid protein derived from the plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with freund and montanide isa720 adjuvants |
| publisher |
American Society for Microbiology |
| publishDate |
2019 |
| url |
http://patua.iec.gov.br//handle/iec/3737 |
| _version_ |
1717584399350890496 |
| score |
11.755432 |