Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two...
| Autor principal: | Domingues, Sheyla Farhayldes Souza |
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| Outros Autores: | Ferreira, Humberto Soares, Muniz, Jos? Augusto Pereira Carneiro, Lima, Ana Kelen Felipe, Ohashi, Ot?vio Mitio, Figueiredo, Jos? Ricardo de, Silva, L?cia Daniel Machado da |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Universidade Federal de Minas Gerais
2019
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/3789 |
| Resumo: |
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The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000?m, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean?SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000?m cut interval) to 300,830+111,460 (at the 500?m cut interval. The mean diameter of the isolated preantral follicles varied from 11.6?m to 27.8?m. |