Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles

Domingues, Sheyla Farhayldes Souza; Ferreira, Humberto Soares; Muniz, Jos? Augusto Pereira Carneiro; Lima, Ana Kelen Felipe; Ohashi, Ot?vio Mitio; Figueiredo, Jos? Ricardo de; Silva, L?cia Daniel Machado da

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Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles

The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two...

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Autor principal:	Domingues, Sheyla Farhayldes Souza
Outros Autores:	Ferreira, Humberto Soares, Muniz, Jos? Augusto Pereira Carneiro, Lima, Ana Kelen Felipe, Ohashi, Ot?vio Mitio, Figueiredo, Jos? Ricardo de, Silva, L?cia Daniel Machado da
Grau:	Artigo
Idioma:	eng
Publicado em:	Universidade Federal de Minas Gerais 2019
Assuntos:	Primatas / anatomia & histologia Cebus / anatomia & histologia Fol?culo Ovariano / citologia Isolamento Reprodutivo Ov?rio / anatomia & histologia
Acesso em linha:	http://patua.iec.gov.br//handle/iec/3789

id	ir-iec-3789
recordtype	dspace
spelling	ir-iec-37892019-06-26T18:14:24Z Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles Isolamento mec?nico de fol?culos ovarianos pr?-antrais de macaca-prego (Cebus apella) Domingues, Sheyla Farhayldes Souza Ferreira, Humberto Soares Muniz, Jos? Augusto Pereira Carneiro Lima, Ana Kelen Felipe Ohashi, Ot?vio Mitio Figueiredo, Jos? Ricardo de Silva, L?cia Daniel Machado da Primatas / anatomia & histologia Cebus / anatomia & histologia Fol?culo Ovariano / citologia Isolamento Reprodutivo Ov?rio / anatomia & histologia The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000?m, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean?SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000?m cut interval) to 300,830+111,460 (at the 500?m cut interval. The mean diameter of the isolated preantral follicles varied from 11.6?m to 27.8?m. Centro Nacional de Primatas (CENP-FUNASA- Ananideua - PA) and the Laborat?rio de Fertiliza??o in vitro (Universidade Federal do Par?, Bel?m-Brazil). 2019-06-25T16:49:23Z 2019-06-25T16:49:23Z 2003 Artigo DOMINGUES, Sheyla Farhayldes Souza et al. Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles. Arquivo Brasileiro de Medicina Veterin?ria e Zootecnia, v. 55, n. 3, p. 301-308, jun. 2003. 1678-4162 http://patua.iec.gov.br//handle/iec/3789 10.1590/S0102-09352003000300009 eng Acesso Aberto application/pdf Universidade Federal de Minas Gerais
institution	Instituto Evandro Chagas (IEC)
collection	PATUA
language	eng
topic	Primatas / anatomia & histologia Cebus / anatomia & histologia Fol?culo Ovariano / citologia Isolamento Reprodutivo Ov?rio / anatomia & histologia
spellingShingle	Primatas / anatomia & histologia Cebus / anatomia & histologia Fol?culo Ovariano / citologia Isolamento Reprodutivo Ov?rio / anatomia & histologia Domingues, Sheyla Farhayldes Souza Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
topic_facet	Primatas / anatomia & histologia Cebus / anatomia & histologia Fol?culo Ovariano / citologia Isolamento Reprodutivo Ov?rio / anatomia & histologia
description	The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000?m, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean?SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000?m cut interval) to 300,830+111,460 (at the 500?m cut interval. The mean diameter of the isolated preantral follicles varied from 11.6?m to 27.8?m.
format	Artigo
author	Domingues, Sheyla Farhayldes Souza
author2	Ferreira, Humberto Soares Muniz, Jos? Augusto Pereira Carneiro Lima, Ana Kelen Felipe Ohashi, Ot?vio Mitio Figueiredo, Jos? Ricardo de Silva, L?cia Daniel Machado da
author2Str	Ferreira, Humberto Soares Muniz, Jos? Augusto Pereira Carneiro Lima, Ana Kelen Felipe Ohashi, Ot?vio Mitio Figueiredo, Jos? Ricardo de Silva, L?cia Daniel Machado da
title	Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
title_short	Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
title_full	Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
title_fullStr	Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
title_full_unstemmed	Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
title_sort	mechanical isolation of capuchin monkey (cebus apella) preantral ovarian follicles
publisher	Universidade Federal de Minas Gerais
publishDate	2019
url	http://patua.iec.gov.br//handle/iec/3789
_version_	1717584510703370240
score	11.653393

Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles

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