Oropouche orthobunyavirus: genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments
Oropouche orthobunyavirus (OROV) has significant impact in public health in Amazon region. This arbovirus is one of the most common causes of febrile illness in Brazil, and is responsible for several epidemics since 1960's. In this study, we sequenced and characterized the complete coding sequences...
| Autor principal: | Nunes, M?rcio Roberto Teixeira |
|---|---|
| Outros Autores: | Souza, William Marciel de, Savji, Nazir, Figueiredo, M?rio Lu?s, Cardoso, Jedson Ferreira, Silva, Sandro Patroca da, Lima, Clayton Pereira Silva de, Vasconcelos, Helena Baldez, Rodrigues, Sueli Guerreiro, Lipkin, W. Ian, Vasconcelos, Pedro Fernando da Costa, Palacios, Gustavo |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Elsevier
2019
|
| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/3837 |
| Resumo: |
|---|
|
Oropouche orthobunyavirus (OROV) has significant impact in public health in Amazon region. This arbovirus is one of the most common causes of febrile illness in Brazil, and is responsible for several epidemics since 1960's. In this study, we sequenced and characterized the complete coding sequences (S-, M- and L-RNA) of 35 OROV isolates from Brazil. Here, we classified 20 strains in genotype I from Par? and Maranh?o states, nine as genotype II from Par? and Rond?nia states confirmed, four classified into genotype III from Acre, Maranh?o, Minas Gerais and Rond?nia states and two genotype IV from Amazonas State. Also, we did not observe reassortment events involving the OROV isolates. In addition, we developed novel RT-PCR tools to identify reassortment events among OROV strains. These data will be useful to better understand the molecular epidemiology and diagnostic of OROV infections. |