Oropouche orthobunyavirus: genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments

Nunes, M?rcio Roberto Teixeira; Souza, William Marciel de; Savji, Nazir; Figueiredo, M?rio Lu?s; Cardoso, Jedson Ferreira; Silva, Sandro Patroca da; Lima, Clayton Pereira Silva de; Vasconcelos, Helena Baldez; Rodrigues, Sueli Guerreiro; Lipkin, W. Ian; Vasconcelos, Pedro Fernando da Costa; Palacios, Gustavo

Artigo

Oropouche orthobunyavirus: genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments

Oropouche orthobunyavirus (OROV) has significant impact in public health in Amazon region. This arbovirus is one of the most common causes of febrile illness in Brazil, and is responsible for several epidemics since 1960's. In this study, we sequenced and characterized the complete coding sequences...

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Autor principal:	Nunes, M?rcio Roberto Teixeira
Outros Autores:	Souza, William Marciel de, Savji, Nazir, Figueiredo, M?rio Lu?s, Cardoso, Jedson Ferreira, Silva, Sandro Patroca da, Lima, Clayton Pereira Silva de, Vasconcelos, Helena Baldez, Rodrigues, Sueli Guerreiro, Lipkin, W. Ian, Vasconcelos, Pedro Fernando da Costa, Palacios, Gustavo
Grau:	Artigo
Idioma:	eng
Publicado em:	Elsevier 2019
Assuntos:	Arbovirus / isolamento & purifica??o Orthobunyavirus / patogenicidade Arbov?rus / gen?tica Gen?tipo Rea??o em Cadeia da Polimerase / m?todos Rearranjo G?nico
Acesso em linha:	http://patua.iec.gov.br//handle/iec/3837

id	ir-iec-3837
recordtype	dspace
spelling	ir-iec-38372019-08-14T17:35:57Z Oropouche orthobunyavirus: genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments Nunes, M?rcio Roberto Teixeira Souza, William Marciel de Savji, Nazir Figueiredo, M?rio Lu?s Cardoso, Jedson Ferreira Silva, Sandro Patroca da Lima, Clayton Pereira Silva de Vasconcelos, Helena Baldez Rodrigues, Sueli Guerreiro Lipkin, W. Ian Vasconcelos, Pedro Fernando da Costa Palacios, Gustavo Arbovirus / isolamento & purifica??o Orthobunyavirus / patogenicidade Arbov?rus / gen?tica Gen?tipo Rea??o em Cadeia da Polimerase / m?todos Rearranjo G?nico Oropouche orthobunyavirus (OROV) has significant impact in public health in Amazon region. This arbovirus is one of the most common causes of febrile illness in Brazil, and is responsible for several epidemics since 1960's. In this study, we sequenced and characterized the complete coding sequences (S-, M- and L-RNA) of 35 OROV isolates from Brazil. Here, we classified 20 strains in genotype I from Par? and Maranh?o states, nine as genotype II from Par? and Rond?nia states confirmed, four classified into genotype III from Acre, Maranh?o, Minas Gerais and Rond?nia states and two genotype IV from Amazonas State. Also, we did not observe reassortment events involving the OROV isolates. In addition, we developed novel RT-PCR tools to identify reassortment events among OROV strains. These data will be useful to better understand the molecular epidemiology and diagnostic of OROV infections. 2019-08-14T17:28:26Z 2019-08-14T17:28:26Z 2019 Artigo NUNES, M?rcio Roberto Teixeira et al. Oropouche orthobunyavirus: genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments. Infection, Genetics and Evolution, v. 68, p. 16-22, Mar. 2019. 1567-1348 http://patua.iec.gov.br//handle/iec/3837 10.1016/j.meegid.2018.11.020 eng Acesso Embargado application/pdf Elsevier
institution	Instituto Evandro Chagas (IEC)
collection	PATUA
language	eng
topic	Arbovirus / isolamento & purifica??o Orthobunyavirus / patogenicidade Arbov?rus / gen?tica Gen?tipo Rea??o em Cadeia da Polimerase / m?todos Rearranjo G?nico
spellingShingle	Arbovirus / isolamento & purifica??o Orthobunyavirus / patogenicidade Arbov?rus / gen?tica Gen?tipo Rea??o em Cadeia da Polimerase / m?todos Rearranjo G?nico Nunes, M?rcio Roberto Teixeira Oropouche orthobunyavirus: genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments
topic_facet	Arbovirus / isolamento & purifica??o Orthobunyavirus / patogenicidade Arbov?rus / gen?tica Gen?tipo Rea??o em Cadeia da Polimerase / m?todos Rearranjo G?nico
description	Oropouche orthobunyavirus (OROV) has significant impact in public health in Amazon region. This arbovirus is one of the most common causes of febrile illness in Brazil, and is responsible for several epidemics since 1960's. In this study, we sequenced and characterized the complete coding sequences (S-, M- and L-RNA) of 35 OROV isolates from Brazil. Here, we classified 20 strains in genotype I from Par? and Maranh?o states, nine as genotype II from Par? and Rond?nia states confirmed, four classified into genotype III from Acre, Maranh?o, Minas Gerais and Rond?nia states and two genotype IV from Amazonas State. Also, we did not observe reassortment events involving the OROV isolates. In addition, we developed novel RT-PCR tools to identify reassortment events among OROV strains. These data will be useful to better understand the molecular epidemiology and diagnostic of OROV infections.
format	Artigo
author	Nunes, M?rcio Roberto Teixeira
author2	Souza, William Marciel de Savji, Nazir Figueiredo, M?rio Lu?s Cardoso, Jedson Ferreira Silva, Sandro Patroca da Lima, Clayton Pereira Silva de Vasconcelos, Helena Baldez Rodrigues, Sueli Guerreiro Lipkin, W. Ian Vasconcelos, Pedro Fernando da Costa Palacios, Gustavo
author2Str	Souza, William Marciel de Savji, Nazir Figueiredo, M?rio Lu?s Cardoso, Jedson Ferreira Silva, Sandro Patroca da Lima, Clayton Pereira Silva de Vasconcelos, Helena Baldez Rodrigues, Sueli Guerreiro Lipkin, W. Ian Vasconcelos, Pedro Fernando da Costa Palacios, Gustavo
title	Oropouche orthobunyavirus: genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments
title_short	Oropouche orthobunyavirus: genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments
title_full	Oropouche orthobunyavirus: genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments
title_fullStr	Oropouche orthobunyavirus: genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments
title_full_unstemmed	Oropouche orthobunyavirus: genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments
title_sort	oropouche orthobunyavirus: genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments
publisher	Elsevier
publishDate	2019
url	http://patua.iec.gov.br//handle/iec/3837
_version_	1717584400149905408
score	11.755432

Oropouche orthobunyavirus: genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments

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