Artigo

Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil

In Fortaleza, the capital of Ceara State, Brazil, the detection rate of tuberculosis (TB) in 2018 was 65.5/100,000 inhabitants with a cure rate of 59.1%, which is higher than the country average. This study investigated the risk factors associated with drug-resistant tuberculosis (DR-TB) and identif...

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Autor principal: Campelo, Thales Alves
Outros Autores: Lima, Luana Nepomuceno Costa, Lima, Karla Val?ria Batista, Silva, Caroliny Soares, Concei??o, Mar?lia Lima da, Barreto, Jos? Antonio Pereira, Mota, Aquiles Paulino Peres, Sancho, Soraya de Oliveira, Frota, Cristiane Cunha
Grau: Artigo
Idioma: eng
Publicado em: Universidade de S?o Paulo 2020
Assuntos:
Tuberculose / diagn?stico
Tuberculose / tratamento farmacol?gico
Fatores de Risco
Tuberculose Resistente a M?ltiplos Medicamentos
Resist?ncia Microbiana a Medicamentos / imunologia
Mycobacterium tuberculosis / isolamento & purifica??o
Fortaleza (CE)
Acesso em linha: http://patua.iec.gov.br//handle/iec/4071
id ir-iec-4071
recordtype dspace
spelling ir-iec-40712020-03-30T17:34:03Z Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil Campelo, Thales Alves Lima, Luana Nepomuceno Costa Lima, Karla Val?ria Batista Silva, Caroliny Soares Concei??o, Mar?lia Lima da Barreto, Jos? Antonio Pereira Mota, Aquiles Paulino Peres Sancho, Soraya de Oliveira Frota, Cristiane Cunha Tuberculose / diagn?stico Tuberculose / tratamento farmacol?gico Fatores de Risco Tuberculose Resistente a M?ltiplos Medicamentos Resist?ncia Microbiana a Medicamentos / imunologia Mycobacterium tuberculosis / isolamento & purifica??o Fortaleza (CE) In Fortaleza, the capital of Ceara State, Brazil, the detection rate of tuberculosis (TB) in 2018 was 65.5/100,000 inhabitants with a cure rate of 59.1%, which is higher than the country average. This study investigated the risk factors associated with drug-resistant tuberculosis (DR-TB) and identified the drug-resistance phenotype and resistance-conferring mutations. The geographic distribution of DR-TB in Fortaleza, Brazil, was also determined. From March 2017 to February 2018, 41 DR-TB isolates and 69 drug-susceptible pulmonary TB isolates were obtained from patients seen at a referral hospital in Fortaleza, Brazil. Samples were subjected to phenotypic and genetic analysis of resistance; the spatial distribution of the participants was also analyzed. Primary resistance was high (50.9%) among participants. The following risk factors for DR were identified: being female ( p = 0.03), having diabetes ( p < 0.01), history of previous TB disease ( p < 0.01), and the number of intra-domiciliary contacts ( p < 0.01). Analysis by multiplex allele-specific polymerase chain reaction detected mutations in the genes katG (65.8%) , rpoB (43.9%), inhA promoter (14.6%), and gyrA (9.8%). Sequencing identified mutations in the the genes katG (75.6%), inhA promoter (19.5%), rpoB (85.4%), and gyrA (100%). There was no mutation in the rrs gene. Spatial analysis showed DR-TB isolates distributed in areas of low socioeconomic status in the city of Fortaleza. Our results emphasized the importance of detecting resistance to TB drugs. The resistance found in the gene gyrA is of concern due to the high number of pre-extensive DR-TB cases in Fortaleza. This work was supported by Funda??o Cearense de Apoio ao Desenvolvimento Cient?fico e Tecnol?gico - FUNCAP (grant N? 178638234); and the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - Brasil (CAPES) - Finance Code 001. 2020-03-30T17:02:34Z 2020-03-30T17:02:34Z 2020 Artigo CAMPELO, Thales Alves et al. Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil. Revista do Instituto de Medicina Tropical, v. 62, p. 1-10, Feb. 2020. 1678-9946 http://patua.iec.gov.br//handle/iec/4071 10.1590/s1678-9946202062004 eng Acesso Aberto application/pdf Universidade de S?o Paulo
institution Instituto Evandro Chagas (IEC)
collection PATUA
language eng
topic Tuberculose / diagn?stico
Tuberculose / tratamento farmacol?gico
Fatores de Risco
Tuberculose Resistente a M?ltiplos Medicamentos
Resist?ncia Microbiana a Medicamentos / imunologia
Mycobacterium tuberculosis / isolamento & purifica??o
Fortaleza (CE)
spellingShingle Tuberculose / diagn?stico
Tuberculose / tratamento farmacol?gico
Fatores de Risco
Tuberculose Resistente a M?ltiplos Medicamentos
Resist?ncia Microbiana a Medicamentos / imunologia
Mycobacterium tuberculosis / isolamento & purifica??o
Fortaleza (CE)
Campelo, Thales Alves
Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil
topic_facet Tuberculose / diagn?stico
Tuberculose / tratamento farmacol?gico
Fatores de Risco
Tuberculose Resistente a M?ltiplos Medicamentos
Resist?ncia Microbiana a Medicamentos / imunologia
Mycobacterium tuberculosis / isolamento & purifica??o
Fortaleza (CE)
description In Fortaleza, the capital of Ceara State, Brazil, the detection rate of tuberculosis (TB) in 2018 was 65.5/100,000 inhabitants with a cure rate of 59.1%, which is higher than the country average. This study investigated the risk factors associated with drug-resistant tuberculosis (DR-TB) and identified the drug-resistance phenotype and resistance-conferring mutations. The geographic distribution of DR-TB in Fortaleza, Brazil, was also determined. From March 2017 to February 2018, 41 DR-TB isolates and 69 drug-susceptible pulmonary TB isolates were obtained from patients seen at a referral hospital in Fortaleza, Brazil. Samples were subjected to phenotypic and genetic analysis of resistance; the spatial distribution of the participants was also analyzed. Primary resistance was high (50.9%) among participants. The following risk factors for DR were identified: being female ( p = 0.03), having diabetes ( p < 0.01), history of previous TB disease ( p < 0.01), and the number of intra-domiciliary contacts ( p < 0.01). Analysis by multiplex allele-specific polymerase chain reaction detected mutations in the genes katG (65.8%) , rpoB (43.9%), inhA promoter (14.6%), and gyrA (9.8%). Sequencing identified mutations in the the genes katG (75.6%), inhA promoter (19.5%), rpoB (85.4%), and gyrA (100%). There was no mutation in the rrs gene. Spatial analysis showed DR-TB isolates distributed in areas of low socioeconomic status in the city of Fortaleza. Our results emphasized the importance of detecting resistance to TB drugs. The resistance found in the gene gyrA is of concern due to the high number of pre-extensive DR-TB cases in Fortaleza.
format Artigo
author Campelo, Thales Alves
author2 Lima, Luana Nepomuceno Costa
Lima, Karla Val?ria Batista
Silva, Caroliny Soares
Concei??o, Mar?lia Lima da
Barreto, Jos? Antonio Pereira
Mota, Aquiles Paulino Peres
Sancho, Soraya de Oliveira
Frota, Cristiane Cunha
author2Str Lima, Luana Nepomuceno Costa
Lima, Karla Val?ria Batista
Silva, Caroliny Soares
Concei??o, Mar?lia Lima da
Barreto, Jos? Antonio Pereira
Mota, Aquiles Paulino Peres
Sancho, Soraya de Oliveira
Frota, Cristiane Cunha
title Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil
title_short Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil
title_full Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil
title_fullStr Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil
title_full_unstemmed Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in northeast Brazil
title_sort molecular characterization of pre-extensive drug resistant mycobacterium tuberculosis in northeast brazil
publisher Universidade de S?o Paulo
publishDate 2020
url http://patua.iec.gov.br//handle/iec/4071
_version_ 1717584454210289664
score 11.674684

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