Genotoxic effect of non-lethal concentrations of minocycline in human glial cell culture
Minocycline has been proposed as a neuroprotective agent with pleiotropic effects on several experimental models of neurodegenerative diseases, including microglial inhibition. However, although most studies have focused on the central actions of minocycline in affecting microglial functions, othe...
| Autor principal: | Puty, Bruna |
|---|---|
| Outros Autores: | Nogueira, Iago C?sar da Costa, Nogueira, Lygia S, Vasconcelos, Carolina Pinheiro, Ara?jo, Teka Mayara Corr?a, Bittencourt, Leonardo Oliveira, Ferreira, Railson de Oliveira, Oliveira, Edivaldo Herculano Correa de, Leal, Walace Gomes, Lima, Rafael Rodrigues |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Elsevier
2020
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/4093 |
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ir-iec-4093 |
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| recordtype |
dspace |
| spelling |
ir-iec-40932020-06-08T17:57:18Z Genotoxic effect of non-lethal concentrations of minocycline in human glial cell culture Puty, Bruna Nogueira, Iago C?sar da Costa Nogueira, Lygia S Vasconcelos, Carolina Pinheiro Ara?jo, Teka Mayara Corr?a Bittencourt, Leonardo Oliveira Ferreira, Railson de Oliveira Oliveira, Edivaldo Herculano Correa de Leal, Walace Gomes Lima, Rafael Rodrigues Sistema Nervoso Central / anatomia & histologia Neuroglia Minociclina / administra??o & dosagem Minociclina / toxicidade Dano ao DNA / gen?tica Citotoxicidade Minocycline has been proposed as a neuroprotective agent with pleiotropic effects on several experimental models of neurodegenerative diseases, including microglial inhibition. However, although most studies have focused on the central actions of minocycline in affecting microglial functions, other central nervous system (CNS) cell types may also be affected by this drug toxicity. Hence, considering that glial cells play a pivotal role on CNS physiology and are the main responsible for neuronal integrity, a comprehensive investigation on the effects of minocycline treatment on human glial cells is mandatory before translational studies to afford neuroprotection in humans. Therefore, we explored the cytotoxic and genotoxic effects of minocycline at different concentrations in glial cells using an in vitro model. To achieve this, U87 glial cell were exposed to 10?50 ?g/mL for 24 h. After exposure, cell viability, general metabolic status and genotoxic assays were performed. No changes were observed in cell viability, however, the general metabolic status decreased over 20 ?g/mL. In addition, although no chromossome aberrations were observed, evidences of genotoxicity, such as increase on micronucleus, buds and bridges, were observed from 10 ?g/mL. These results suggest that minocycline may induce genotoxic effects even at concentrations considered previously safe and should be used with caution in translational studies. This study was financed in part by the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior?Brazil (CAPES)?Finance Code 001 and supported by the Brazilian National Council for Scientific and Technological Development (CNPq). 2020-06-08T13:25:39Z 2020-06-08T13:25:39Z 2020 Artigo PUTY, Bruna et al. Genotoxic effect of non-lethal concentrations of minocycline in human glial cell culture. Biomedicine & Pharmacotherapy, v. 128, n. 110285, 2020. 1950-6007 http://patua.iec.gov.br//handle/iec/4093 10.1016/j.biopha.2020.110285 eng Acesso Aberto application/pdf Elsevier |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Sistema Nervoso Central / anatomia & histologia Neuroglia Minociclina / administra??o & dosagem Minociclina / toxicidade Dano ao DNA / gen?tica Citotoxicidade |
| spellingShingle |
Sistema Nervoso Central / anatomia & histologia Neuroglia Minociclina / administra??o & dosagem Minociclina / toxicidade Dano ao DNA / gen?tica Citotoxicidade Puty, Bruna Genotoxic effect of non-lethal concentrations of minocycline in human glial cell culture |
| topic_facet |
Sistema Nervoso Central / anatomia & histologia Neuroglia Minociclina / administra??o & dosagem Minociclina / toxicidade Dano ao DNA / gen?tica Citotoxicidade |
| description |
Minocycline has been proposed as a neuroprotective agent with pleiotropic effects on several experimental
models of neurodegenerative diseases, including microglial inhibition. However, although most studies have
focused on the central actions of minocycline in affecting microglial functions, other central nervous system
(CNS) cell types may also be affected by this drug toxicity. Hence, considering that glial cells play a pivotal role
on CNS physiology and are the main responsible for neuronal integrity, a comprehensive investigation on the
effects of minocycline treatment on human glial cells is mandatory before translational studies to afford neuroprotection in humans. Therefore, we explored the cytotoxic and genotoxic effects of minocycline at different
concentrations in glial cells using an in vitro model. To achieve this, U87 glial cell were exposed to 10?50 ?g/mL
for 24 h. After exposure, cell viability, general metabolic status and genotoxic assays were performed. No
changes were observed in cell viability, however, the general metabolic status decreased over 20 ?g/mL. In
addition, although no chromossome aberrations were observed, evidences of genotoxicity, such as increase on
micronucleus, buds and bridges, were observed from 10 ?g/mL. These results suggest that minocycline may
induce genotoxic effects even at concentrations considered previously safe and should be used with caution in
translational studies. |
| format |
Artigo |
| author |
Puty, Bruna |
| author2 |
Nogueira, Iago C?sar da Costa Nogueira, Lygia S Vasconcelos, Carolina Pinheiro Ara?jo, Teka Mayara Corr?a Bittencourt, Leonardo Oliveira Ferreira, Railson de Oliveira Oliveira, Edivaldo Herculano Correa de Leal, Walace Gomes Lima, Rafael Rodrigues |
| author2Str |
Nogueira, Iago C?sar da Costa Nogueira, Lygia S Vasconcelos, Carolina Pinheiro Ara?jo, Teka Mayara Corr?a Bittencourt, Leonardo Oliveira Ferreira, Railson de Oliveira Oliveira, Edivaldo Herculano Correa de Leal, Walace Gomes Lima, Rafael Rodrigues |
| title |
Genotoxic effect of non-lethal concentrations of minocycline in human glial cell culture |
| title_short |
Genotoxic effect of non-lethal concentrations of minocycline in human glial cell culture |
| title_full |
Genotoxic effect of non-lethal concentrations of minocycline in human glial cell culture |
| title_fullStr |
Genotoxic effect of non-lethal concentrations of minocycline in human glial cell culture |
| title_full_unstemmed |
Genotoxic effect of non-lethal concentrations of minocycline in human glial cell culture |
| title_sort |
genotoxic effect of non-lethal concentrations of minocycline in human glial cell culture |
| publisher |
Elsevier |
| publishDate |
2020 |
| url |
http://patua.iec.gov.br//handle/iec/4093 |
| _version_ |
1717584537031016448 |
| score |
11.755432 |