Insect-specific viruses and arboviruses in adult male culicids from Midwestern Brazil

Ferreira, Raquel da Silva; Cruz, Lucin?ia Claudia de Toni Aquino da; Souza, Vilma Jucineide; Neves, Nilvanei Aparecido da Silva; Souza, Victor Costa de; Franco Filho, Luciano Chaves; Lemos, Poliana da Silva; Lima, Clayton Pereira Silva de; Naveca, Felipe Gomes; Atanaka, Marina; Nunes, Marcio Roberto Teixeira; Slhessarenko, Renata Dezengrini

Artigo

Insect-specific viruses and arboviruses in adult male culicids from Midwestern Brazil

Viruses were identified from male anthropophilic mosquitoes from Mato Grosso (MT) State, Midwest Brazil from February 2017 to January 2018. Mosquitoes tested included Aedes (Stegomyia) aegypti (1139 males; 84 pools), Culex quinquefasciatus (9426 males; 179 pools), Culex sp. (3 males; 3 pools) and Ps...

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Autor principal:	Ferreira, Raquel da Silva
Outros Autores:	Cruz, Lucin?ia Claudia de Toni Aquino da, Souza, Vilma Jucineide, Neves, Nilvanei Aparecido da Silva, Souza, Victor Costa de, Franco Filho, Luciano Chaves, Lemos, Poliana da Silva, Lima, Clayton Pereira Silva de, Naveca, Felipe Gomes, Atanaka, Marina, Nunes, Marcio Roberto Teixeira, Slhessarenko, Renata Dezengrini
Grau:	Artigo
Idioma:	eng
Publicado em:	Elsevier 2020
Assuntos:	Alphavirus / isolamento & purifica??o Insetos / virologia Culicidae / virologia Arbovirus / patogenicidade Mato Grosso (MT) Flavivirus / isolamento & purifica??o
Acesso em linha:	http://patua.iec.gov.br//handle/iec/4184

id	ir-iec-4184
recordtype	dspace
spelling	ir-iec-41842020-10-14T01:12:19Z Insect-specific viruses and arboviruses in adult male culicids from Midwestern Brazil Ferreira, Raquel da Silva Cruz, Lucin?ia Claudia de Toni Aquino da Souza, Vilma Jucineide Neves, Nilvanei Aparecido da Silva Souza, Victor Costa de Franco Filho, Luciano Chaves Lemos, Poliana da Silva Lima, Clayton Pereira Silva de Naveca, Felipe Gomes Atanaka, Marina Nunes, Marcio Roberto Teixeira Slhessarenko, Renata Dezengrini Alphavirus / isolamento & purifica??o Insetos / virologia Culicidae / virologia Arbovirus / patogenicidade Mato Grosso (MT) Flavivirus / isolamento & purifica??o Viruses were identified from male anthropophilic mosquitoes from Mato Grosso (MT) State, Midwest Brazil from February 2017 to January 2018. Mosquitoes tested included Aedes (Stegomyia) aegypti (1139 males; 84 pools), Culex quinquefasciatus (9426 males; 179 pools), Culex sp. (3 males; 3 pools) and Psorophora albigenu (1 male; 1 pool) collected from four cities of MT. Pools were subjected to viral RNA extraction followed by RT-PCRs specific for ten flaviviruses, five alphaviruses and Simbu serogroup of orthobunyaviruses. Positive pools were passaged three times in VERO cells (alphavirus and orthobunyavirus) or C6/36 cells (flavivirus), with isolates confirmed through RT-PCR and nucleotide sequencing. We detected pools positive for Ilh?us (1 pool), dengue serotype 4 (1), Mayaro (12), equine encephalitis virus (1) yellow fever (1), Oropouche (2), Zika (4) and chikungunya (12) viruses. High throughput sequencing of arbovirus positive pools identified 35 insect-specific viruses (ISVs) from the families Circoviridae (2), Parvoviridae (2), Totiviridae (1), Flaviviridae (1), Iflaviridae (2), Mesoniviridae (4), Nodaviridae (2), Luteoviridae (1), Phasmaviridae (1) Phenuiviridae (2), Rhabdoviridae (2), Orthomyxoviridae (1), Xinmoviridae (1), and unclassified Bunyavirales (1), unclassified Picornavirales (3), unclassified Riboviria (4) and taxon Negevirus (5). From these, five novel viruses were tentatively named Mojica circovirus, Kuia iflavirus, Muxirum negevirus, Lambada picorna-like virus and Tacuru picorna-like virus. Our findings underscore the diversity and wide geographical distribution of ISVs and arboviruses infecting male culicids. 2020-10-13T18:54:27Z 2020-10-13T18:54:27Z 2020 Artigo FERREIRA, Raquel da Silva et al. Insect-specific viruses and arboviruses in adult male culicids from Midwestern Brazil. Infection, Genetics and Evolution, v. 85, n. 104561, p. xx, Nov. 2020. 1567-1348 http://patua.iec.gov.br//handle/iec/4184 10.1016/j.meegid.2020.104561 eng Acesso Embargado application/pdf Elsevier
institution	Instituto Evandro Chagas (IEC)
collection	PATUA
language	eng
topic	Alphavirus / isolamento & purifica??o Insetos / virologia Culicidae / virologia Arbovirus / patogenicidade Mato Grosso (MT) Flavivirus / isolamento & purifica??o
spellingShingle	Alphavirus / isolamento & purifica??o Insetos / virologia Culicidae / virologia Arbovirus / patogenicidade Mato Grosso (MT) Flavivirus / isolamento & purifica??o Ferreira, Raquel da Silva Insect-specific viruses and arboviruses in adult male culicids from Midwestern Brazil
topic_facet	Alphavirus / isolamento & purifica??o Insetos / virologia Culicidae / virologia Arbovirus / patogenicidade Mato Grosso (MT) Flavivirus / isolamento & purifica??o
description	Viruses were identified from male anthropophilic mosquitoes from Mato Grosso (MT) State, Midwest Brazil from February 2017 to January 2018. Mosquitoes tested included Aedes (Stegomyia) aegypti (1139 males; 84 pools), Culex quinquefasciatus (9426 males; 179 pools), Culex sp. (3 males; 3 pools) and Psorophora albigenu (1 male; 1 pool) collected from four cities of MT. Pools were subjected to viral RNA extraction followed by RT-PCRs specific for ten flaviviruses, five alphaviruses and Simbu serogroup of orthobunyaviruses. Positive pools were passaged three times in VERO cells (alphavirus and orthobunyavirus) or C6/36 cells (flavivirus), with isolates confirmed through RT-PCR and nucleotide sequencing. We detected pools positive for Ilh?us (1 pool), dengue serotype 4 (1), Mayaro (12), equine encephalitis virus (1) yellow fever (1), Oropouche (2), Zika (4) and chikungunya (12) viruses. High throughput sequencing of arbovirus positive pools identified 35 insect-specific viruses (ISVs) from the families Circoviridae (2), Parvoviridae (2), Totiviridae (1), Flaviviridae (1), Iflaviridae (2), Mesoniviridae (4), Nodaviridae (2), Luteoviridae (1), Phasmaviridae (1) Phenuiviridae (2), Rhabdoviridae (2), Orthomyxoviridae (1), Xinmoviridae (1), and unclassified Bunyavirales (1), unclassified Picornavirales (3), unclassified Riboviria (4) and taxon Negevirus (5). From these, five novel viruses were tentatively named Mojica circovirus, Kuia iflavirus, Muxirum negevirus, Lambada picorna-like virus and Tacuru picorna-like virus. Our findings underscore the diversity and wide geographical distribution of ISVs and arboviruses infecting male culicids.
format	Artigo
author	Ferreira, Raquel da Silva
author2	Cruz, Lucin?ia Claudia de Toni Aquino da Souza, Vilma Jucineide Neves, Nilvanei Aparecido da Silva Souza, Victor Costa de Franco Filho, Luciano Chaves Lemos, Poliana da Silva Lima, Clayton Pereira Silva de Naveca, Felipe Gomes Atanaka, Marina Nunes, Marcio Roberto Teixeira Slhessarenko, Renata Dezengrini
author2Str	Cruz, Lucin?ia Claudia de Toni Aquino da Souza, Vilma Jucineide Neves, Nilvanei Aparecido da Silva Souza, Victor Costa de Franco Filho, Luciano Chaves Lemos, Poliana da Silva Lima, Clayton Pereira Silva de Naveca, Felipe Gomes Atanaka, Marina Nunes, Marcio Roberto Teixeira Slhessarenko, Renata Dezengrini
title	Insect-specific viruses and arboviruses in adult male culicids from Midwestern Brazil
title_short	Insect-specific viruses and arboviruses in adult male culicids from Midwestern Brazil
title_full	Insect-specific viruses and arboviruses in adult male culicids from Midwestern Brazil
title_fullStr	Insect-specific viruses and arboviruses in adult male culicids from Midwestern Brazil
title_full_unstemmed	Insect-specific viruses and arboviruses in adult male culicids from Midwestern Brazil
title_sort	insect-specific viruses and arboviruses in adult male culicids from midwestern brazil
publisher	Elsevier
publishDate	2020
url	http://patua.iec.gov.br//handle/iec/4184
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score	11.755432

Insect-specific viruses and arboviruses in adult male culicids from Midwestern Brazil

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