OBJECTIVE: To trace the infection profile of Mayaro virus (MAYV) and Chikungunya virus (CHIKV) in four different cell
lineages. MATERIALS AND METHODS: The isolates of these viruses were used to infect cultures of the following cell
lineages ? C6/36, VERO, BHK-21, and LLC-MK2 ? under the multiplicity of infection of 1 PFU/cell, which were monitored
for up to 96 h post-infection (hpi) by phase-contrast microscopy to register the cytopathic effect (CPE); and in addition were
subjected to indirect immunofluorescence (iIF) and quantitative reverse transcription polymerase chain reaction (qRT-PCR)
assays for virus antigen detection and RNA load determination, respectively. RESULTS: For both viruses, VERO and BHK-21
cells had CPE of 24 hpi, LLC-MK2 cells presented CPE from 48 hpi, and C6/36 did not present any CPE. In the iIF assays,
all cells were already positive at 6 hpi, with the exception of CHIKV-infected LLC-MK2 and BHK-21 cells. Finally, qRT-PCR
assays showed that in general cells infected with MAYV presented higher RNA load, although CHIKV-infected LLC-MK2
cells showed the highest RNA load. CONCLUSION: By evaluating the behavior of these arboviruses in different types of
cell cultures, including some not commonly used in the laboratory diagnostic routine, the present study provides not only
a better comprehension of the replication kinetics of MAYV and CHIKV, but also opportunities for the optimization of their
diagnosis in biological samples.
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