Polymorphism in the IL-1? promoter is associated with IgG antibody response to circumsporozoite protein repeats of Plasmodium vivax
BACKGROUND: It is well established that infection by Plasmodium vivax is a result of host-parasite interactions. In the present study, association with the IL1/IL2 cytokine profiles, anticircumsporozoite protein antibody levels and parasitic loads was evaluated in individuals naturally infected with...
| Autor principal: | Capobianco, Marcela Petrolini |
|---|---|
| Outros Autores: | Cassiano, Gustavo Capatti, Storti-Melo, Luciane Moreno, Pimenta, Tamirys Sim?o, Rodrigues, Ana Paula Drummond, Arruda, Jos? Eduardo Gomes, Pinto, Marcia Ribeiro, Baptista, Andrea Regina de Souza, Pratt-Riccio, Lilian Rose, Bonini-Domingos, Cl?udia Regina, Oliveira-Ferreira, Joseli de, Machado, Ricardo Luiz Dantas |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Oxford University Press
2020
|
| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/4201 |
| id |
ir-iec-4201 |
|---|---|
| recordtype |
dspace |
| spelling |
ir-iec-42012020-11-17T17:08:33Z Polymorphism in the IL-1? promoter is associated with IgG antibody response to circumsporozoite protein repeats of Plasmodium vivax Capobianco, Marcela Petrolini Cassiano, Gustavo Capatti Storti-Melo, Luciane Moreno Pimenta, Tamirys Sim?o Rodrigues, Ana Paula Drummond Arruda, Jos? Eduardo Gomes Pinto, Marcia Ribeiro Baptista, Andrea Regina de Souza Pratt-Riccio, Lilian Rose Bonini-Domingos, Cl?udia Regina Oliveira-Ferreira, Joseli de Machado, Ricardo Luiz Dantas Plasmodium vivax / parasitologia Polimorfismo Gen?tico Citocinas Prote?na Circunsporozo?ta Anticorpos Interleucina-1beta / gen?tica Goian?sia do Par? (PA) BACKGROUND: It is well established that infection by Plasmodium vivax is a result of host-parasite interactions. In the present study, association with the IL1/IL2 cytokine profiles, anticircumsporozoite protein antibody levels and parasitic loads was evaluated in individuals naturally infected with P. vivax in an endemic area of the Brazilian Amazon. METHODS: Molecular diagnosis of P. vivax and variants was performed using the PCR-RFLP method and IL1B -511C>T, IL2 -330T>G and IL2+114T>G polymorphisms were identified using PCR-RFLP and allele-specific PCR. IL-1? and IL-2 cytokine levels were detected by flow cytometry and circumsporozoite protein (CSP) antibodies were measured by ELISA. RESULTS: Three variants of P. vivax CSP were identified and VK247 was found to be the most frequent. However, the prevalence and magnitude of IgG antibodies were higher for the VK210 variant. Furthermore, the antibody response to the CSP variants was not associated with the presence of the variant in the infection. Significant differences were observed between the single nucleotide polymorphism (SNP) -511T>C in the IL1B gene and levels of antibodies to the VK247 and P. vivax-like variants, but there were no associations between SNPs in IL1 and IL2 genes and their plasma products. CONCLUSIONS: Individuals with the rs16944 CC genotype in the IL1? gene have higher antibody levels to the CSP of P. vivax of VK247 and P. vivax-like variants. Conselho Nacional para o Desenvolvimento Cient?fico e Tecnol?gico (CNPq) ; Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, Brazil (CAPES ? DS and PDSE) ? Finance Code 001 2020-11-16T18:14:20Z 2020-11-16T18:14:20Z 2020 Artigo CAPOBIANCO, Marcela Petrolini et al. Polymorphism in the IL-1? promoter is associated with IgG antibody response to circumsporozoite protein repeats of Plasmodium vivax. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 114, n. 11, p. 858-865, Nov. 2020. 1878-3503 http://patua.iec.gov.br//handle/iec/4201 10.1093/trstmh/traa055 eng Acesso Aberto application/pdf Oxford University Press |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Plasmodium vivax / parasitologia Polimorfismo Gen?tico Citocinas Prote?na Circunsporozo?ta Anticorpos Interleucina-1beta / gen?tica Goian?sia do Par? (PA) |
| spellingShingle |
Plasmodium vivax / parasitologia Polimorfismo Gen?tico Citocinas Prote?na Circunsporozo?ta Anticorpos Interleucina-1beta / gen?tica Goian?sia do Par? (PA) Capobianco, Marcela Petrolini Polymorphism in the IL-1? promoter is associated with IgG antibody response to circumsporozoite protein repeats of Plasmodium vivax |
| topic_facet |
Plasmodium vivax / parasitologia Polimorfismo Gen?tico Citocinas Prote?na Circunsporozo?ta Anticorpos Interleucina-1beta / gen?tica Goian?sia do Par? (PA) |
| description |
BACKGROUND: It is well established that infection by Plasmodium vivax is a result of host-parasite interactions. In the present study, association with the IL1/IL2 cytokine profiles, anticircumsporozoite protein antibody levels and parasitic loads was evaluated in individuals naturally infected with P. vivax in an endemic area of the Brazilian Amazon. METHODS: Molecular diagnosis of P. vivax and variants was performed using the PCR-RFLP method and IL1B -511C>T, IL2 -330T>G and IL2+114T>G polymorphisms were identified using PCR-RFLP and allele-specific PCR. IL-1? and IL-2 cytokine levels were detected by flow cytometry and circumsporozoite protein (CSP) antibodies were measured by ELISA. RESULTS: Three variants of P. vivax CSP were identified and VK247 was found to be the most frequent. However, the prevalence and magnitude of IgG antibodies were higher for the VK210 variant. Furthermore, the antibody response to the CSP variants was not associated with the presence of the variant in the infection. Significant differences were observed between the single nucleotide polymorphism (SNP) -511T>C in the IL1B gene and levels of antibodies to the VK247 and P. vivax-like variants, but there were no associations between SNPs in IL1 and IL2 genes and their plasma products. CONCLUSIONS: Individuals with the rs16944 CC genotype in the IL1? gene have higher antibody levels to the CSP of P. vivax of VK247 and P. vivax-like variants. |
| format |
Artigo |
| author |
Capobianco, Marcela Petrolini |
| author2 |
Cassiano, Gustavo Capatti Storti-Melo, Luciane Moreno Pimenta, Tamirys Sim?o Rodrigues, Ana Paula Drummond Arruda, Jos? Eduardo Gomes Pinto, Marcia Ribeiro Baptista, Andrea Regina de Souza Pratt-Riccio, Lilian Rose Bonini-Domingos, Cl?udia Regina Oliveira-Ferreira, Joseli de Machado, Ricardo Luiz Dantas |
| author2Str |
Cassiano, Gustavo Capatti Storti-Melo, Luciane Moreno Pimenta, Tamirys Sim?o Rodrigues, Ana Paula Drummond Arruda, Jos? Eduardo Gomes Pinto, Marcia Ribeiro Baptista, Andrea Regina de Souza Pratt-Riccio, Lilian Rose Bonini-Domingos, Cl?udia Regina Oliveira-Ferreira, Joseli de Machado, Ricardo Luiz Dantas |
| title |
Polymorphism in the IL-1? promoter is associated with IgG antibody response to circumsporozoite protein repeats of Plasmodium vivax |
| title_short |
Polymorphism in the IL-1? promoter is associated with IgG antibody response to circumsporozoite protein repeats of Plasmodium vivax |
| title_full |
Polymorphism in the IL-1? promoter is associated with IgG antibody response to circumsporozoite protein repeats of Plasmodium vivax |
| title_fullStr |
Polymorphism in the IL-1? promoter is associated with IgG antibody response to circumsporozoite protein repeats of Plasmodium vivax |
| title_full_unstemmed |
Polymorphism in the IL-1? promoter is associated with IgG antibody response to circumsporozoite protein repeats of Plasmodium vivax |
| title_sort |
polymorphism in the il-1? promoter is associated with igg antibody response to circumsporozoite protein repeats of plasmodium vivax |
| publisher |
Oxford University Press |
| publishDate |
2020 |
| url |
http://patua.iec.gov.br//handle/iec/4201 |
| _version_ |
1717584539107196928 |
| score |
11.653393 |