Extensive chromosomal fissions and repetitive DNA accumulation shaped the atypical karyotypes of two Ramphastidae (Aves: Piciformes) species

Kretschmer, Rafael; Furo, Ivanete de Oliveira; Cioffi, Marcelo de Belo; Gunski, Ricardo Jos?; Garnero, Anal?a Del Valle; O'Brien, Patricia C. M; Ferguson-Smith, Malcolm A; Freitas, Thales Renato Ochotorena de; Oliveira, Edivaldo Herculano Corr?a de

Artigo

Extensive chromosomal fissions and repetitive DNA accumulation shaped the atypical karyotypes of two Ramphastidae (Aves: Piciformes) species

In contrast to the ?avian-like? diploid number (2n = 80), most toucans and aracaris (Piciformes: Ramphastidae) have divergent karyotypes, exhibiting a higher 2n. To identify the chromosomal rearrangements that shaped the karyotype of these species, we applied chicken macrochromosome paints 1?10 and...

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Autor principal:	Kretschmer, Rafael
Outros Autores:	Furo, Ivanete de Oliveira, Cioffi, Marcelo de Belo, Gunski, Ricardo Jos?, Garnero, Anal?a Del Valle, O'Brien, Patricia C. M, Ferguson-Smith, Malcolm A, Freitas, Thales Renato Ochotorena de, Oliveira, Edivaldo Herculano Corr?a de
Grau:	Artigo
Idioma:	eng
Publicado em:	Oxford University Press 2021
Assuntos:	Aves / gen?tica Colora??o Cromoss?mica / m?todos Cariotipagem / m?todos Cari?tipo Aracaris Tucano
Acesso em linha:	http://patua.iec.gov.br//handle/iec/4228

id	ir-iec-4228
recordtype	dspace
spelling	ir-iec-42282021-03-08T16:29:09Z Extensive chromosomal fissions and repetitive DNA accumulation shaped the atypical karyotypes of two Ramphastidae (Aves: Piciformes) species Kretschmer, Rafael Furo, Ivanete de Oliveira Cioffi, Marcelo de Belo Gunski, Ricardo Jos? Garnero, Anal?a Del Valle O'Brien, Patricia C. M Ferguson-Smith, Malcolm A Freitas, Thales Renato Ochotorena de Oliveira, Edivaldo Herculano Corr?a de Aves / gen?tica Colora??o Cromoss?mica / m?todos Cariotipagem / m?todos Cari?tipo Aracaris Tucano In contrast to the ?avian-like? diploid number (2n = 80), most toucans and aracaris (Piciformes: Ramphastidae) have divergent karyotypes, exhibiting a higher 2n. To identify the chromosomal rearrangements that shaped the karyotype of these species, we applied chicken macrochromosome paints 1?10 and 11 microsatellite sequences to the chromosomes of two representative species, Pteroglossus inscriptus and Ramphastos tucannus tucannus. Paints of chicken chromosomes revealed that at least the first five ancestral chromosomes have undergone fissions, and a fusion between a segment of chicken chromosome 1 and a segment from chromosome 3 occurred in both species. The microsatellite sequences were accumulated mainly in the Z chromosome and in several microchromosomes in both species. These results suggest that the genomes of the Ramphastidae have been shaped by extensive fissions and repetitive DNA accumulation as the main driving forces leading to the higher 2n as found in these species. Furthermore, our results suggest that the putative ancestral karyotype of Ramphastidae already had a high diploid number, probably close to 2n = 112, similar to that observed in P. inscriptus and R. t. tucannus. 2021-01-29T13:22:22Z 2021-01-29T13:22:22Z 2020 Artigo KRETSCHMER, Rafael et al. Extensive chromosomal fissions and repetitive DNA accumulation shaped the atypical karyotypes of two Ramphastidae (Aves: Piciformes) species. Biological Journal of the Linnean Society, v. 130, n. 4, p. 839-849, Aug. 2020. 1095-8312 http://patua.iec.gov.br//handle/iec/4228 10.1093/biolinnean/blaa086 eng Acesso Embargado application/pdf Oxford University Press
institution	Instituto Evandro Chagas (IEC)
collection	PATUA
language	eng
topic	Aves / gen?tica Colora??o Cromoss?mica / m?todos Cariotipagem / m?todos Cari?tipo Aracaris Tucano
spellingShingle	Aves / gen?tica Colora??o Cromoss?mica / m?todos Cariotipagem / m?todos Cari?tipo Aracaris Tucano Kretschmer, Rafael Extensive chromosomal fissions and repetitive DNA accumulation shaped the atypical karyotypes of two Ramphastidae (Aves: Piciformes) species
topic_facet	Aves / gen?tica Colora??o Cromoss?mica / m?todos Cariotipagem / m?todos Cari?tipo Aracaris Tucano
description	In contrast to the ?avian-like? diploid number (2n = 80), most toucans and aracaris (Piciformes: Ramphastidae) have divergent karyotypes, exhibiting a higher 2n. To identify the chromosomal rearrangements that shaped the karyotype of these species, we applied chicken macrochromosome paints 1?10 and 11 microsatellite sequences to the chromosomes of two representative species, Pteroglossus inscriptus and Ramphastos tucannus tucannus. Paints of chicken chromosomes revealed that at least the first five ancestral chromosomes have undergone fissions, and a fusion between a segment of chicken chromosome 1 and a segment from chromosome 3 occurred in both species. The microsatellite sequences were accumulated mainly in the Z chromosome and in several microchromosomes in both species. These results suggest that the genomes of the Ramphastidae have been shaped by extensive fissions and repetitive DNA accumulation as the main driving forces leading to the higher 2n as found in these species. Furthermore, our results suggest that the putative ancestral karyotype of Ramphastidae already had a high diploid number, probably close to 2n = 112, similar to that observed in P. inscriptus and R. t. tucannus.
format	Artigo
author	Kretschmer, Rafael
author2	Furo, Ivanete de Oliveira Cioffi, Marcelo de Belo Gunski, Ricardo Jos? Garnero, Anal?a Del Valle O'Brien, Patricia C. M Ferguson-Smith, Malcolm A Freitas, Thales Renato Ochotorena de Oliveira, Edivaldo Herculano Corr?a de
author2Str	Furo, Ivanete de Oliveira Cioffi, Marcelo de Belo Gunski, Ricardo Jos? Garnero, Anal?a Del Valle O'Brien, Patricia C. M Ferguson-Smith, Malcolm A Freitas, Thales Renato Ochotorena de Oliveira, Edivaldo Herculano Corr?a de
title	Extensive chromosomal fissions and repetitive DNA accumulation shaped the atypical karyotypes of two Ramphastidae (Aves: Piciformes) species
title_short	Extensive chromosomal fissions and repetitive DNA accumulation shaped the atypical karyotypes of two Ramphastidae (Aves: Piciformes) species
title_full	Extensive chromosomal fissions and repetitive DNA accumulation shaped the atypical karyotypes of two Ramphastidae (Aves: Piciformes) species
title_fullStr	Extensive chromosomal fissions and repetitive DNA accumulation shaped the atypical karyotypes of two Ramphastidae (Aves: Piciformes) species
title_full_unstemmed	Extensive chromosomal fissions and repetitive DNA accumulation shaped the atypical karyotypes of two Ramphastidae (Aves: Piciformes) species
title_sort	extensive chromosomal fissions and repetitive dna accumulation shaped the atypical karyotypes of two ramphastidae (aves: piciformes) species
publisher	Oxford University Press
publishDate	2021
url	http://patua.iec.gov.br//handle/iec/4228
_version_	1717584430312194048
score	11.755432

Extensive chromosomal fissions and repetitive DNA accumulation shaped the atypical karyotypes of two Ramphastidae (Aves: Piciformes) species

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