Artigo

Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys

The absence of an adequate animal model for studies has limited the understanding of congenital Zika syndrome (CZS) in humans during the outbreak in America. In this study, we used squirrel monkeys (Saimiri collinsi), a neotropical primate (which mimics the stages of human pregnancy), as a model of...

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Autor principal: Alcantara, Bianca Nascimento de
Outros Autores: Imbeloni, Aline Amaral, Durans, Darlene de Brito Simith, Ara?jo, Marialva Tereza Ferreira de, Cruz, Ermelinda do Ros?rio Moutinho da, Carvalho, Carlos Alberto Marques de, Mendon?a, Maria Helena Rodrigues de, Sousa, Jorge Rodrigues de, Moraes, Adriana Freitas, Martins Filho, Arnaldo Jorge, Lima, Maria de Lourdes Gomes, Amador Neto, Orlando Pereira, Chiang, Jannifer Oliveira, Scalercio, Sarah Raphaella Rocha de Azevedo, Carneiro, Liliane Almeida, Quaresma, Juarez Ant?nio Sim?es, Vasconcelos, Pedro Fernando da Costa, Medeiros, Daniele Barbosa de Almeida
Grau: Artigo
Idioma: eng
Publicado em: Nature Research 2021
Assuntos:
Primatas / anatomia & histologia
Saimiri / anatomia & histologia
Primatas / les?es
Infec??o por Zika virus / veterin?ria
C?rebro / les?es
Exame Neurol?gico / veterin?ria
Acesso em linha: http://patua.iec.gov.br//handle/iec/4271
id ir-iec-4271
recordtype dspace
spelling ir-iec-42712021-03-29T18:36:01Z Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys Alcantara, Bianca Nascimento de Imbeloni, Aline Amaral Durans, Darlene de Brito Simith Ara?jo, Marialva Tereza Ferreira de Cruz, Ermelinda do Ros?rio Moutinho da Carvalho, Carlos Alberto Marques de Mendon?a, Maria Helena Rodrigues de Sousa, Jorge Rodrigues de Moraes, Adriana Freitas Martins Filho, Arnaldo Jorge Lima, Maria de Lourdes Gomes Amador Neto, Orlando Pereira Chiang, Jannifer Oliveira Scalercio, Sarah Raphaella Rocha de Azevedo Carneiro, Liliane Almeida Quaresma, Juarez Ant?nio Sim?es Vasconcelos, Pedro Fernando da Costa Medeiros, Daniele Barbosa de Almeida Primatas / anatomia & histologia Saimiri / anatomia & histologia Primatas / les?es Infec??o por Zika virus / veterin?ria C?rebro / les?es Exame Neurol?gico / veterin?ria The absence of an adequate animal model for studies has limited the understanding of congenital Zika syndrome (CZS) in humans during the outbreak in America. In this study, we used squirrel monkeys (Saimiri collinsi), a neotropical primate (which mimics the stages of human pregnancy), as a model of Zika virus (ZIKV) infection. Seven pregnant female squirrel monkeys were experimentally infected at three different gestational stages, and we were able reproduce a broad range of clinical manifestations of ZIKV lesions observed in newborn humans. Histopathological and immunohistochemical analyses of early-infected newborns (2/4) revealed damage to various areas of the brain and ZIKV antigens in the cytoplasm of neurons and glial cells, indicative of CZS. The changes caused by ZIKV infection were intrauterine developmental delay, ventriculomegaly, simplified brain gyri, vascular impairment and neuroprogenitor cell dysfunction. Our data show that the ZIKV infection outcome in squirrel monkeys is similar to that in humans, indicating that this model can be used to help answer questions about the effect of ZIKV infection on neuroembryonic development and the morphological changes induced by CZS. This study was supported by grants from CNPq, CAPES (Zika Fast-Track, 440405/2016-5) and FINEP agencies to P.F.C.V. (CNPq: 303999/2016-0) and D.B.A.M. (CNPq: 306581/2016-7). 2021-03-29T14:01:37Z 2021-03-29T14:01:37Z 2021 Artigo ALCANTARA, Bianca Nascimento de et al. Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys. Scientific Reports, v. 11, n. 6099, 2021. 2045-2322 http://patua.iec.gov.br//handle/iec/4271 10.1038/s41598-021-85571-1 eng Acesso Aberto application/pdf Nature Research
institution Instituto Evandro Chagas (IEC)
collection PATUA
language eng
topic Primatas / anatomia & histologia
Saimiri / anatomia & histologia
Primatas / les?es
Infec??o por Zika virus / veterin?ria
C?rebro / les?es
Exame Neurol?gico / veterin?ria
spellingShingle Primatas / anatomia & histologia
Saimiri / anatomia & histologia
Primatas / les?es
Infec??o por Zika virus / veterin?ria
C?rebro / les?es
Exame Neurol?gico / veterin?ria
Alcantara, Bianca Nascimento de
Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
topic_facet Primatas / anatomia & histologia
Saimiri / anatomia & histologia
Primatas / les?es
Infec??o por Zika virus / veterin?ria
C?rebro / les?es
Exame Neurol?gico / veterin?ria
description The absence of an adequate animal model for studies has limited the understanding of congenital Zika syndrome (CZS) in humans during the outbreak in America. In this study, we used squirrel monkeys (Saimiri collinsi), a neotropical primate (which mimics the stages of human pregnancy), as a model of Zika virus (ZIKV) infection. Seven pregnant female squirrel monkeys were experimentally infected at three different gestational stages, and we were able reproduce a broad range of clinical manifestations of ZIKV lesions observed in newborn humans. Histopathological and immunohistochemical analyses of early-infected newborns (2/4) revealed damage to various areas of the brain and ZIKV antigens in the cytoplasm of neurons and glial cells, indicative of CZS. The changes caused by ZIKV infection were intrauterine developmental delay, ventriculomegaly, simplified brain gyri, vascular impairment and neuroprogenitor cell dysfunction. Our data show that the ZIKV infection outcome in squirrel monkeys is similar to that in humans, indicating that this model can be used to help answer questions about the effect of ZIKV infection on neuroembryonic development and the morphological changes induced by CZS.
format Artigo
author Alcantara, Bianca Nascimento de
author2 Imbeloni, Aline Amaral
Durans, Darlene de Brito Simith
Ara?jo, Marialva Tereza Ferreira de
Cruz, Ermelinda do Ros?rio Moutinho da
Carvalho, Carlos Alberto Marques de
Mendon?a, Maria Helena Rodrigues de
Sousa, Jorge Rodrigues de
Moraes, Adriana Freitas
Martins Filho, Arnaldo Jorge
Lima, Maria de Lourdes Gomes
Amador Neto, Orlando Pereira
Chiang, Jannifer Oliveira
Scalercio, Sarah Raphaella Rocha de Azevedo
Carneiro, Liliane Almeida
Quaresma, Juarez Ant?nio Sim?es
Vasconcelos, Pedro Fernando da Costa
Medeiros, Daniele Barbosa de Almeida
author2Str Imbeloni, Aline Amaral
Durans, Darlene de Brito Simith
Ara?jo, Marialva Tereza Ferreira de
Cruz, Ermelinda do Ros?rio Moutinho da
Carvalho, Carlos Alberto Marques de
Mendon?a, Maria Helena Rodrigues de
Sousa, Jorge Rodrigues de
Moraes, Adriana Freitas
Martins Filho, Arnaldo Jorge
Lima, Maria de Lourdes Gomes
Amador Neto, Orlando Pereira
Chiang, Jannifer Oliveira
Scalercio, Sarah Raphaella Rocha de Azevedo
Carneiro, Liliane Almeida
Quaresma, Juarez Ant?nio Sim?es
Vasconcelos, Pedro Fernando da Costa
Medeiros, Daniele Barbosa de Almeida
title Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
title_short Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
title_full Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
title_fullStr Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
title_full_unstemmed Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
title_sort histopathological lesions of congenital zika syndrome in newborn squirrel monkeys
publisher Nature Research
publishDate 2021
url http://patua.iec.gov.br//handle/iec/4271
_version_ 1717584333994196992
score 11.755432

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