Antibodies against the Plasmodium vivax apical membrane antigen 1 from the Belem strain share common epitopes among other worldwide variants

Fran?a, Ana Caroline Barbosa; Fran?oso, K?tia Sanches; Marques, Rodolfo Ferreira; Trossini, Gustavo H. G; Gomes, Renan A; P?voa, Marinete Marins; Cunha, Maristela G; Silveira, Eduardo L. V; Soares, Irene S

Artigo

Antibodies against the Plasmodium vivax apical membrane antigen 1 from the Belem strain share common epitopes among other worldwide variants

Malaria is a human parasitic disease distributed in many tropical countries and caused by various Plasmodium species. Plasmodium vivax has the largest geographical distribution of the Plasmodium species and is predominant in the Americas, including Brazil. Only a small number of P. vivax vaccine for...

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Autor principal:	Fran?a, Ana Caroline Barbosa
Outros Autores:	Fran?oso, K?tia Sanches, Marques, Rodolfo Ferreira, Trossini, Gustavo H. G, Gomes, Renan A, P?voa, Marinete Marins, Cunha, Maristela G, Silveira, Eduardo L. V, Soares, Irene S
Grau:	Artigo
Idioma:	eng
Publicado em:	Frontiers Media 2021
Assuntos:	Mal?ria Vivax / patologia Plasmodium vivax / parasitologia Ant?genos Polimorfismo Gen?tico Vacinas Antimal?ricas
Acesso em linha:	http://patua.iec.gov.br//handle/iec/4287

id	ir-iec-4287
recordtype	dspace
spelling	ir-iec-42872021-04-13T15:13:47Z Antibodies against the Plasmodium vivax apical membrane antigen 1 from the Belem strain share common epitopes among other worldwide variants Fran?a, Ana Caroline Barbosa Fran?oso, K?tia Sanches Marques, Rodolfo Ferreira Trossini, Gustavo H. G Gomes, Renan A P?voa, Marinete Marins Cunha, Maristela G Silveira, Eduardo L. V Soares, Irene S Mal?ria Vivax / patologia Plasmodium vivax / parasitologia Ant?genos Polimorfismo Gen?tico Vacinas Antimal?ricas Malaria is a human parasitic disease distributed in many tropical countries and caused by various Plasmodium species. Plasmodium vivax has the largest geographical distribution of the Plasmodium species and is predominant in the Americas, including Brazil. Only a small number of P. vivax vaccine formulations have successfully reached clinical trials relative to their P. falciparum counterparts. One of the candidate antigens for a blood-stage P. vivax vaccine is apical membrane antigen 1 (PvAMA-1). Due to the worldwide distribution of Plasmodium parasites, a high degree of variability has been detected in this antigen sequence, representing a considerable challenge to the development of a universal vaccine against malaria. In this study, we evaluated how PvAMA-1 polymorphisms influence vaccine-derived immune responses in P. vivax malaria. To this end, we expressed 9 recombinant protein representatives of different PvAMA-1 allelic variants in the yeast Pichia pastoris: Belem, Chesson I, Sal-1, Indonesia XIX, SK0814, TC103, PNG_05_ESP, PNG_62_MU, and PNG_68_MAS. After protein expression and purification, we evaluated the breadth of the immune responses derived from malaria-exposed individuals from the Amazon region. From 611 serum samples of malaria-exposed individuals, 53.68% of them reacted against the PvAMA-1 Belem through ELISA. Positive samples were further tested against recombinant proteins representing the other PvAMA-1 allelic variants. Whereas Sal-1, Chesson I and SK0814 variants were highly recognized by tested serum samples, Indonesia XIX, TC103, PNG_05_ESP, PNG_62_MU, and PNG_68_MAS were only slightly recognized. Moreover, polyclonal sera derived from C57BL/6 mice immunized with the PvAMA-1 Belem protein predominantly recognized Belem, Sal-1, Chesson I, SK0814, and Indonesia XIX through ELISA. Last, ELISA-based competition assays demonstrated that a previous interaction between anti-Belem polyclonal serum and Sal-1, Chesson I, SK0814, or Indonesia XIX proteins could further inhibit antibody binding to the Belem variant. Our human and mouse data suggest the presence of common epitopes or cross-reactivity between Belem, Sal-1, Chesson I, and SK0814 variants. Although the PvAMA-1 Belem variant induces strain-transcendent antibodies, PvAMA-1 variants from Thailand and Papua New Guinea may need to be included in a universal vaccine formulation to achieve protection against P. vivax malaria. This work was supported by grants from Fundac??o de Amparo ? Pesquisa do Estado de S?o Paulo (FAPESP 2012/ 13032-5 and 2016/50108-0), Instituto Nacional de Cie?ncia e Tecnologia de Vacinas (INCTV), National Counsel of Technological and Scientific Development (CNPq ? 555.654/2009-5), and Para? State Research Foundation (FAPESPA ? ICAAF 005/2011). 2021-04-11T20:30:51Z 2021-04-11T20:30:51Z 2021 Artigo FRAN?A, Ana Caroline Barbosa et al. Antibodies against the Plasmodium vivax apical membrane antigen 1 from the Belem strain share common epitopes among other worldwide variants. Frontiers in Cellular and Infection Microbiology, v. 11, n. 616230, p. 1-13, Mar. 2021. 2235-2988 http://patua.iec.gov.br//handle/iec/4287 eng Acesso Aberto application/pdf Frontiers Media
institution	Instituto Evandro Chagas (IEC)
collection	PATUA
language	eng
topic	Mal?ria Vivax / patologia Plasmodium vivax / parasitologia Ant?genos Polimorfismo Gen?tico Vacinas Antimal?ricas
spellingShingle	Mal?ria Vivax / patologia Plasmodium vivax / parasitologia Ant?genos Polimorfismo Gen?tico Vacinas Antimal?ricas Fran?a, Ana Caroline Barbosa Antibodies against the Plasmodium vivax apical membrane antigen 1 from the Belem strain share common epitopes among other worldwide variants
topic_facet	Mal?ria Vivax / patologia Plasmodium vivax / parasitologia Ant?genos Polimorfismo Gen?tico Vacinas Antimal?ricas
description	Malaria is a human parasitic disease distributed in many tropical countries and caused by various Plasmodium species. Plasmodium vivax has the largest geographical distribution of the Plasmodium species and is predominant in the Americas, including Brazil. Only a small number of P. vivax vaccine formulations have successfully reached clinical trials relative to their P. falciparum counterparts. One of the candidate antigens for a blood-stage P. vivax vaccine is apical membrane antigen 1 (PvAMA-1). Due to the worldwide distribution of Plasmodium parasites, a high degree of variability has been detected in this antigen sequence, representing a considerable challenge to the development of a universal vaccine against malaria. In this study, we evaluated how PvAMA-1 polymorphisms influence vaccine-derived immune responses in P. vivax malaria. To this end, we expressed 9 recombinant protein representatives of different PvAMA-1 allelic variants in the yeast Pichia pastoris: Belem, Chesson I, Sal-1, Indonesia XIX, SK0814, TC103, PNG_05_ESP, PNG_62_MU, and PNG_68_MAS. After protein expression and purification, we evaluated the breadth of the immune responses derived from malaria-exposed individuals from the Amazon region. From 611 serum samples of malaria-exposed individuals, 53.68% of them reacted against the PvAMA-1 Belem through ELISA. Positive samples were further tested against recombinant proteins representing the other PvAMA-1 allelic variants. Whereas Sal-1, Chesson I and SK0814 variants were highly recognized by tested serum samples, Indonesia XIX, TC103, PNG_05_ESP, PNG_62_MU, and PNG_68_MAS were only slightly recognized. Moreover, polyclonal sera derived from C57BL/6 mice immunized with the PvAMA-1 Belem protein predominantly recognized Belem, Sal-1, Chesson I, SK0814, and Indonesia XIX through ELISA. Last, ELISA-based competition assays demonstrated that a previous interaction between anti-Belem polyclonal serum and Sal-1, Chesson I, SK0814, or Indonesia XIX proteins could further inhibit antibody binding to the Belem variant. Our human and mouse data suggest the presence of common epitopes or cross-reactivity between Belem, Sal-1, Chesson I, and SK0814 variants. Although the PvAMA-1 Belem variant induces strain-transcendent antibodies, PvAMA-1 variants from Thailand and Papua New Guinea may need to be included in a universal vaccine formulation to achieve protection against P. vivax malaria.
format	Artigo
author	Fran?a, Ana Caroline Barbosa
author2	Fran?oso, K?tia Sanches Marques, Rodolfo Ferreira Trossini, Gustavo H. G Gomes, Renan A P?voa, Marinete Marins Cunha, Maristela G Silveira, Eduardo L. V Soares, Irene S
author2Str	Fran?oso, K?tia Sanches Marques, Rodolfo Ferreira Trossini, Gustavo H. G Gomes, Renan A P?voa, Marinete Marins Cunha, Maristela G Silveira, Eduardo L. V Soares, Irene S
title	Antibodies against the Plasmodium vivax apical membrane antigen 1 from the Belem strain share common epitopes among other worldwide variants
title_short	Antibodies against the Plasmodium vivax apical membrane antigen 1 from the Belem strain share common epitopes among other worldwide variants
title_full	Antibodies against the Plasmodium vivax apical membrane antigen 1 from the Belem strain share common epitopes among other worldwide variants
title_fullStr	Antibodies against the Plasmodium vivax apical membrane antigen 1 from the Belem strain share common epitopes among other worldwide variants
title_full_unstemmed	Antibodies against the Plasmodium vivax apical membrane antigen 1 from the Belem strain share common epitopes among other worldwide variants
title_sort	antibodies against the plasmodium vivax apical membrane antigen 1 from the belem strain share common epitopes among other worldwide variants
publisher	Frontiers Media
publishDate	2021
url	http://patua.iec.gov.br//handle/iec/4287
_version_	1717584355791994880
score	11.755432

Antibodies against the Plasmodium vivax apical membrane antigen 1 from the Belem strain share common epitopes among other worldwide variants

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