Artigo

Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil

The occurrence of triatomine species, their bloodmeal sources and the discrete typing units (DTUs) of Trypanosoma cruzi isolated from them were determined in different municipalities of the state of Rio Grande do Norte, Brazil. Triatomine captures were carried out in the rural areas of 23 municipali...

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Autor principal: Honorato, Nathan Ravi Medeiros
Outros Autores: Silva, Andress Noronha Barbosa da, Negreiros, Christiane Carlos Ara?jo de, Aguiar, L?cia Maria Abrantes, Marli?re, Newmar Pinto, Souza, Rita de C?ssia Moreira de, Guimar?es, Ricardo Jos? de Paula Souza e, Galv?o, L?cia Maria da Cunha, C?mara, Ant?nia Cl?udia J?come da
Grau: Artigo
Idioma: eng
Publicado em: Elsevier 2021
Assuntos:
Doen?a de Chagas
Trypanosoma cruzi / parasitologia
Triatominae / anatomia & histologia
Varia??o Gen?tica
Comportamento Alimentar
Coinfec??o / sangue
Zona Rural
Rio Grande do Norte (BR)
Acesso em linha: http://patua.iec.gov.br//handle/iec/4314
id ir-iec-4314
recordtype dspace
spelling ir-iec-43142021-05-25T18:34:38Z Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil Honorato, Nathan Ravi Medeiros Silva, Andress Noronha Barbosa da Negreiros, Christiane Carlos Ara?jo de Aguiar, L?cia Maria Abrantes Marli?re, Newmar Pinto Souza, Rita de C?ssia Moreira de Guimar?es, Ricardo Jos? de Paula Souza e Galv?o, L?cia Maria da Cunha C?mara, Ant?nia Cl?udia J?come da Doen?a de Chagas Trypanosoma cruzi / parasitologia Triatominae / anatomia & histologia Varia??o Gen?tica Comportamento Alimentar Coinfec??o / sangue Zona Rural Rio Grande do Norte (BR) The occurrence of triatomine species, their bloodmeal sources and the discrete typing units (DTUs) of Trypanosoma cruzi isolated from them were determined in different municipalities of the state of Rio Grande do Norte, Brazil. Triatomine captures were carried out in the rural areas of 23 municipalities. The genotyping of T. cruzi isolates was performed using the mitochondrial cytochrome c oxidase subunit 2 (coii) gene, the D7 region of the 24S? rDNA, and the spliced leader intergenic region (SL-IR). Five triatomine species were captured, and the most frequent was Triatoma brasiliensis (84.3%; 916/1086), which was found in 16 of the 23 municipalities surveyed, and infested all types of environment investigated. The TcI DTU was found in all mesoregions surveyed in 51.5% (17/33) of the culture-positive samples. In contrast, TcII (9.1%; 3/33) was detected in the Central mesoregion, while TcIII (27.3%; 9/33) was found in all mesoregions. The geographic distribution and spatial overlap of different DTUs was inferred using the superposition of the radius of occurrence of isolates and using ecological niche distribution modelling. Triatoma brasiliensis was found infected in all mesoregions and with all three T. cruzi DTUs, including mixed infections. With regard to bloodmeal sources, the DNA of rodents was found in triatomines infected with either TcI or TcIII, while that of domestic animals and humans was associated with both single and mixed infections. Our findings demonstrate that different DTUs of T. cruzi are widely dispersed among triatomines in our study area. The association of T. brasiliensis with several different mammalian hosts, as well as overlapping areas with different DTUs, suggests that this triatomine species may have an important role as a vector in both anthropic and sylvatic environments. 2021-05-24T18:54:15Z 2021-05-24T18:54:15Z 2021 Artigo HONORATO, Nathan Ravi Medeiros et al. Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil. Acta Tropica, v. 220, n. xx. p. xx, Aug. 2021. 0001-706X http://patua.iec.gov.br//handle/iec/4314 10.1016/j.actatropica.2021.105950 eng Acesso Embargado application/pdf Elsevier
institution Instituto Evandro Chagas (IEC)
collection PATUA
language eng
topic Doen?a de Chagas
Trypanosoma cruzi / parasitologia
Triatominae / anatomia & histologia
Varia??o Gen?tica
Comportamento Alimentar
Coinfec??o / sangue
Zona Rural
Rio Grande do Norte (BR)
spellingShingle Doen?a de Chagas
Trypanosoma cruzi / parasitologia
Triatominae / anatomia & histologia
Varia??o Gen?tica
Comportamento Alimentar
Coinfec??o / sangue
Zona Rural
Rio Grande do Norte (BR)
Honorato, Nathan Ravi Medeiros
Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil
topic_facet Doen?a de Chagas
Trypanosoma cruzi / parasitologia
Triatominae / anatomia & histologia
Varia??o Gen?tica
Comportamento Alimentar
Coinfec??o / sangue
Zona Rural
Rio Grande do Norte (BR)
description The occurrence of triatomine species, their bloodmeal sources and the discrete typing units (DTUs) of Trypanosoma cruzi isolated from them were determined in different municipalities of the state of Rio Grande do Norte, Brazil. Triatomine captures were carried out in the rural areas of 23 municipalities. The genotyping of T. cruzi isolates was performed using the mitochondrial cytochrome c oxidase subunit 2 (coii) gene, the D7 region of the 24S? rDNA, and the spliced leader intergenic region (SL-IR). Five triatomine species were captured, and the most frequent was Triatoma brasiliensis (84.3%; 916/1086), which was found in 16 of the 23 municipalities surveyed, and infested all types of environment investigated. The TcI DTU was found in all mesoregions surveyed in 51.5% (17/33) of the culture-positive samples. In contrast, TcII (9.1%; 3/33) was detected in the Central mesoregion, while TcIII (27.3%; 9/33) was found in all mesoregions. The geographic distribution and spatial overlap of different DTUs was inferred using the superposition of the radius of occurrence of isolates and using ecological niche distribution modelling. Triatoma brasiliensis was found infected in all mesoregions and with all three T. cruzi DTUs, including mixed infections. With regard to bloodmeal sources, the DNA of rodents was found in triatomines infected with either TcI or TcIII, while that of domestic animals and humans was associated with both single and mixed infections. Our findings demonstrate that different DTUs of T. cruzi are widely dispersed among triatomines in our study area. The association of T. brasiliensis with several different mammalian hosts, as well as overlapping areas with different DTUs, suggests that this triatomine species may have an important role as a vector in both anthropic and sylvatic environments.
format Artigo
author Honorato, Nathan Ravi Medeiros
author2 Silva, Andress Noronha Barbosa da
Negreiros, Christiane Carlos Ara?jo de
Aguiar, L?cia Maria Abrantes
Marli?re, Newmar Pinto
Souza, Rita de C?ssia Moreira de
Guimar?es, Ricardo Jos? de Paula Souza e
Galv?o, L?cia Maria da Cunha
C?mara, Ant?nia Cl?udia J?come da
author2Str Silva, Andress Noronha Barbosa da
Negreiros, Christiane Carlos Ara?jo de
Aguiar, L?cia Maria Abrantes
Marli?re, Newmar Pinto
Souza, Rita de C?ssia Moreira de
Guimar?es, Ricardo Jos? de Paula Souza e
Galv?o, L?cia Maria da Cunha
C?mara, Ant?nia Cl?udia J?come da
title Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil
title_short Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil
title_full Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil
title_fullStr Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil
title_full_unstemmed Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil
title_sort triatomine and trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern brazil
publisher Elsevier
publishDate 2021
url http://patua.iec.gov.br//handle/iec/4314
_version_ 1717584485306859520
score 11.755432

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