The usefulness of a duplex RT-qPCR during the recent yellow fever brazilian epidemic: surveillance of vaccine adverse events, epizootics and vectors
Abstract: From 2016 to 2018, Brazil faced the biggest yellow fever (YF) outbreak in the last 80 years, representing a risk of YF reurbanization, especially in megacities. Along with this challenge, the mass administration of the fractionated YF vaccine dose in a na?ve population brought another c...
| Autor principal: | Queiroz, Alice Louize Nunes |
|---|---|
| Outros Autores: | Barros, Rafael S, Silva, Sandro Patroca da, Rodrigues, Daniela Sueli Guerreiro, Cruz, Ana Cec?lia Ribeiro, Santos, Fl?via B. dos, Vasconcelos, Pedro Fernando da Costa, Tesh, Robert B, Nunes, Bruno Tardelli Diniz |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
MDPI
2021
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/4331 |
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ir-iec-4331 |
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ir-iec-43312021-06-28T12:23:55Z The usefulness of a duplex RT-qPCR during the recent yellow fever brazilian epidemic: surveillance of vaccine adverse events, epizootics and vectors Queiroz, Alice Louize Nunes Barros, Rafael S Silva, Sandro Patroca da Rodrigues, Daniela Sueli Guerreiro Cruz, Ana Cec?lia Ribeiro Santos, Fl?via B. dos Vasconcelos, Pedro Fernando da Costa Tesh, Robert B Nunes, Bruno Tardelli Diniz Febre Amarela / patologia Vacina contra Febre Amarela / efeitos adversos Rea??o em Cadeia da Polimerase / m?todos Epidemias Abstract: From 2016 to 2018, Brazil faced the biggest yellow fever (YF) outbreak in the last 80 years, representing a risk of YF reurbanization, especially in megacities. Along with this challenge, the mass administration of the fractionated YF vaccine dose in a na?ve population brought another concern: the possibility to increase YF adverse events associated with viscerotropic (YEL-AVD) or neurological disease (YEL-AND). For this reason, we developed a quantitative real time RT-PCR (RT-qPCR) assay based on a duplex TaqMan protocol to distinguish broad-spectrum infections caused by wild-type yellow fever virus (YFV) strain from adverse events following immunization (AEFI) by 17DD strain during the vaccination campaign used to contain this outbreak. A rapid and more accurate RT-qPCR assay to diagnose YFV was established, being able to detect even different YFV genotypes and geographic strains that circulate in Central and South America. Moreover, after testing around 1400 samples from human cases, non-human primates and mosquitoes, we detected just two YEL-AVD cases, confirmed by sequencing, during the massive vaccination in Brazilian Southeast region, showing lower incidence than AEFI as expected. We are grateful to the National Council for Scientific and Technologic Development Agency-CNPq (processes 306581/2016-7 and 303999/2016-0) for financial support of the study 2021-06-21T17:01:37Z 2021-06-21T17:01:37Z 2021 Artigo QUEIROZ, Alice Louize Nunes et al. The usefulness of a duplex RT-qPCR during the recent yellow fever brazilian epidemic: surveillance of vaccine adverse events, epizootics and vectors. Pathogens, v. 10, n. 6. p. 1-16, June 2021. 2076-0817 http://patua.iec.gov.br//handle/iec/4331 10.3390/pathogens10060693 eng Acesso Aberto application/pdf MDPI |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Febre Amarela / patologia Vacina contra Febre Amarela / efeitos adversos Rea??o em Cadeia da Polimerase / m?todos Epidemias |
| spellingShingle |
Febre Amarela / patologia Vacina contra Febre Amarela / efeitos adversos Rea??o em Cadeia da Polimerase / m?todos Epidemias Queiroz, Alice Louize Nunes The usefulness of a duplex RT-qPCR during the recent yellow fever brazilian epidemic: surveillance of vaccine adverse events, epizootics and vectors |
| topic_facet |
Febre Amarela / patologia Vacina contra Febre Amarela / efeitos adversos Rea??o em Cadeia da Polimerase / m?todos Epidemias |
| description |
Abstract: From 2016 to 2018, Brazil faced the biggest yellow fever (YF) outbreak in the last 80 years,
representing a risk of YF reurbanization, especially in megacities. Along with this challenge, the
mass administration of the fractionated YF vaccine dose in a na?ve population brought another
concern: the possibility to increase YF adverse events associated with viscerotropic (YEL-AVD) or
neurological disease (YEL-AND). For this reason, we developed a quantitative real time RT-PCR
(RT-qPCR) assay based on a duplex TaqMan protocol to distinguish broad-spectrum infections caused
by wild-type yellow fever virus (YFV) strain from adverse events following immunization (AEFI)
by 17DD strain during the vaccination campaign used to contain this outbreak. A rapid and more
accurate RT-qPCR assay to diagnose YFV was established, being able to detect even different YFV
genotypes and geographic strains that circulate in Central and South America. Moreover, after testing
around 1400 samples from human cases, non-human primates and mosquitoes, we detected just two
YEL-AVD cases, confirmed by sequencing, during the massive vaccination in Brazilian Southeast
region, showing lower incidence than AEFI as expected. |
| format |
Artigo |
| author |
Queiroz, Alice Louize Nunes |
| author2 |
Barros, Rafael S Silva, Sandro Patroca da Rodrigues, Daniela Sueli Guerreiro Cruz, Ana Cec?lia Ribeiro Santos, Fl?via B. dos Vasconcelos, Pedro Fernando da Costa Tesh, Robert B Nunes, Bruno Tardelli Diniz |
| author2Str |
Barros, Rafael S Silva, Sandro Patroca da Rodrigues, Daniela Sueli Guerreiro Cruz, Ana Cec?lia Ribeiro Santos, Fl?via B. dos Vasconcelos, Pedro Fernando da Costa Tesh, Robert B Nunes, Bruno Tardelli Diniz |
| title |
The usefulness of a duplex RT-qPCR during the recent yellow fever brazilian epidemic: surveillance of vaccine adverse events, epizootics and vectors |
| title_short |
The usefulness of a duplex RT-qPCR during the recent yellow fever brazilian epidemic: surveillance of vaccine adverse events, epizootics and vectors |
| title_full |
The usefulness of a duplex RT-qPCR during the recent yellow fever brazilian epidemic: surveillance of vaccine adverse events, epizootics and vectors |
| title_fullStr |
The usefulness of a duplex RT-qPCR during the recent yellow fever brazilian epidemic: surveillance of vaccine adverse events, epizootics and vectors |
| title_full_unstemmed |
The usefulness of a duplex RT-qPCR during the recent yellow fever brazilian epidemic: surveillance of vaccine adverse events, epizootics and vectors |
| title_sort |
usefulness of a duplex rt-qpcr during the recent yellow fever brazilian epidemic: surveillance of vaccine adverse events, epizootics and vectors |
| publisher |
MDPI |
| publishDate |
2021 |
| url |
http://patua.iec.gov.br//handle/iec/4331 |
| _version_ |
1717584382717329408 |
| score |
11.675088 |