Guava (Psidium guajava L., Myrtaceae) includes many applications, important
biological activities associated with methods used in plants, being the most prominent
essential oil in its leaves. As leaves of two varieties of red and white Psidium guajava
were collected in different locations of Itacoatiara - AM, their samples were prepared
and deposited in the herbarium of the State University of Amazonas - UEA and their
leaves subjected to anatomical description of their own secretors. and histochemical
characterization. The essential oils from the aerial parts of these genotypes were
extracted by hydrodistillation in Clevenger type apparatus for 4 hours. Analysis by gas
chromatography coupled to mass spectrometry (GC-MS) allowed the identification of
43 compounds in red (PGV) and 35 in white (PGB). Among these compounds,
Limonene, caryophyllene, and 1,8-cineol are of recognized biological activity in the
literature. The Elisa plate microdilution technique was used to verify the antimicrobial
activity of the oils, using LB for bacteria and YPD for fungi. PGV oils were effective
against Salmonella and S. aureus bacteria, at a minimum concentration of 25μL / mL
and PGB under E. coli, with a minimum concentration of 50μL / mL, however, both
varieties were not effective in all tested fungi.
|
