Improvement of a PCR test to diagnose infection by Mansonella ozzardi
Introduction: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. Te common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia. Methods: In o...
| Autor principal: | Vera, Luana Janaína Souza |
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| Outros Autores: | Basano, Sérgio de Almeida, Camargo, Juliana de Souza Almeida Aranha, França, Andonai Krauze de, Godoi Matos Ferreira, Ricardo de, Casseb, Almeida Andrade, Medeiros, Jansen Fernandes de, Fontes, Gilberto, Camargo, Luis Marcelo Aranha |
| Grau: | Artigo |
| Idioma: | pt_BR |
| Publicado em: |
Revista da Sociedade Brasileira de Medicina Tropical
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/15158 |
| Resumo: |
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Introduction: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. Te common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia. Methods: In order to increase diagnosis efcacy, the PCR technique was improved. Results and Conclusions: PCR demonstrated the best performance, with sensitivity and negative predictive values (NPV) of 100%, followed by blood fltration through membrane filters, which showed a sensitivity of 88.9% and a NPV of 84.6%, when compared to thick blood smears. |