Improvement of a PCR test to diagnose infection by Mansonella ozzardi
Introduction: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. Te common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia. Methods: In o...
| Autor principal: | Vera, Luana Janaína Souza |
|---|---|
| Outros Autores: | Basano, Sérgio de Almeida, Camargo, Juliana de Souza Almeida Aranha, França, Andonai Krauze de, Godoi Matos Ferreira, Ricardo de, Casseb, Almeida Andrade, Medeiros, Jansen Fernandes de, Fontes, Gilberto, Camargo, Luis Marcelo Aranha |
| Grau: | Artigo |
| Idioma: | pt_BR |
| Publicado em: |
Revista da Sociedade Brasileira de Medicina Tropical
2020
|
| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/15158 |
| id |
oai:repositorio:1-15158 |
|---|---|
| recordtype |
dspace |
| spelling |
oai:repositorio:1-15158 Improvement of a PCR test to diagnose infection by Mansonella ozzardi Adequação da técnica da PCR para diagnóstico de infecção de Mansonella ozzardi Vera, Luana Janaína Souza Basano, Sérgio de Almeida Camargo, Juliana de Souza Almeida Aranha França, Andonai Krauze de Godoi Matos Ferreira, Ricardo de Casseb, Almeida Andrade Medeiros, Jansen Fernandes de Fontes, Gilberto Camargo, Luis Marcelo Aranha Blood Smear Clinical Clinical Effectiveness Controlled Study Cost Effectiveness Analysis Diagnostic Test Diagnostic Test Accuracy Study Giemsa Stain Gold Standard Human Intermethod Comparison Mansonella Ozzardi Mansonelliasis Membrane Filter Nonhuman Nucleotide Sequence Performance Measurement System Polycarbonate Membrane Filter Polymerase Chain Reaction Predictive Value Sensitivity And Specificity Mansonella Ozzardi Nucleopolyhedrovirus Introduction: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. Te common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia. Methods: In order to increase diagnosis efcacy, the PCR technique was improved. Results and Conclusions: PCR demonstrated the best performance, with sensitivity and negative predictive values (NPV) of 100%, followed by blood fltration through membrane filters, which showed a sensitivity of 88.9% and a NPV of 84.6%, when compared to thick blood smears. 2020-05-07T14:08:25Z 2020-05-07T14:08:25Z 2011 Artigo https://repositorio.inpa.gov.br/handle/1/15158 10.1590/S0037-86822011000300023 pt_BR Volume 44, Número 3, Pags. 380-382 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Revista da Sociedade Brasileira de Medicina Tropical |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
pt_BR |
| topic |
Blood Smear Clinical Clinical Effectiveness Controlled Study Cost Effectiveness Analysis Diagnostic Test Diagnostic Test Accuracy Study Giemsa Stain Gold Standard Human Intermethod Comparison Mansonella Ozzardi Mansonelliasis Membrane Filter Nonhuman Nucleotide Sequence Performance Measurement System Polycarbonate Membrane Filter Polymerase Chain Reaction Predictive Value Sensitivity And Specificity Mansonella Ozzardi Nucleopolyhedrovirus |
| spellingShingle |
Blood Smear Clinical Clinical Effectiveness Controlled Study Cost Effectiveness Analysis Diagnostic Test Diagnostic Test Accuracy Study Giemsa Stain Gold Standard Human Intermethod Comparison Mansonella Ozzardi Mansonelliasis Membrane Filter Nonhuman Nucleotide Sequence Performance Measurement System Polycarbonate Membrane Filter Polymerase Chain Reaction Predictive Value Sensitivity And Specificity Mansonella Ozzardi Nucleopolyhedrovirus Vera, Luana Janaína Souza Improvement of a PCR test to diagnose infection by Mansonella ozzardi |
| topic_facet |
Blood Smear Clinical Clinical Effectiveness Controlled Study Cost Effectiveness Analysis Diagnostic Test Diagnostic Test Accuracy Study Giemsa Stain Gold Standard Human Intermethod Comparison Mansonella Ozzardi Mansonelliasis Membrane Filter Nonhuman Nucleotide Sequence Performance Measurement System Polycarbonate Membrane Filter Polymerase Chain Reaction Predictive Value Sensitivity And Specificity Mansonella Ozzardi Nucleopolyhedrovirus |
| description |
Introduction: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. Te common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia. Methods: In order to increase diagnosis efcacy, the PCR technique was improved. Results and Conclusions: PCR demonstrated the best performance, with sensitivity and negative predictive values (NPV) of 100%, followed by blood fltration through membrane filters, which showed a sensitivity of 88.9% and a NPV of 84.6%, when compared to thick blood smears. |
| format |
Artigo |
| author |
Vera, Luana Janaína Souza |
| author2 |
Basano, Sérgio de Almeida Camargo, Juliana de Souza Almeida Aranha França, Andonai Krauze de Godoi Matos Ferreira, Ricardo de Casseb, Almeida Andrade Medeiros, Jansen Fernandes de Fontes, Gilberto Camargo, Luis Marcelo Aranha |
| author2Str |
Basano, Sérgio de Almeida Camargo, Juliana de Souza Almeida Aranha França, Andonai Krauze de Godoi Matos Ferreira, Ricardo de Casseb, Almeida Andrade Medeiros, Jansen Fernandes de Fontes, Gilberto Camargo, Luis Marcelo Aranha |
| title |
Improvement of a PCR test to diagnose infection by Mansonella ozzardi |
| title_short |
Improvement of a PCR test to diagnose infection by Mansonella ozzardi |
| title_full |
Improvement of a PCR test to diagnose infection by Mansonella ozzardi |
| title_fullStr |
Improvement of a PCR test to diagnose infection by Mansonella ozzardi |
| title_full_unstemmed |
Improvement of a PCR test to diagnose infection by Mansonella ozzardi |
| title_sort |
improvement of a pcr test to diagnose infection by mansonella ozzardi |
| publisher |
Revista da Sociedade Brasileira de Medicina Tropical |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/15158 |
| _version_ |
1787144695480057856 |
| score |
11.755432 |