Successful xenogeneic germ cell transplantation from Jundia catfish (Rhamdia quelen) into adult Nile tilapia (Oreochromis niloticus) testes
Fish germ cell transplantation presents several important potential applications for aquaculture, including the preservation of germplasm from endangered fish species with high genetic and commercial values. Using this technique in studies developed in our laboratory with adult male Nile tilapias (O...
| Autor principal: | Silva, Martinho de Almeida E. |
|---|---|
| Outros Autores: | Costa, Guilherme M.J., Lacerda, Samyra Maria Santos N.Nassif, Brandão-Dias, Pedro Ferreira Pinto, Kalapothakis, Evanguedes, Silva Júnior, Alaion Fonseca da, Alvarenga, Érika Ramos de, França, Luiz Renato de |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
General and Comparative Endocrinology
2020
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| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/15816 |
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oai:repositorio:1-15816 |
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oai:repositorio:1-15816 Successful xenogeneic germ cell transplantation from Jundia catfish (Rhamdia quelen) into adult Nile tilapia (Oreochromis niloticus) testes Silva, Martinho de Almeida E. Costa, Guilherme M.J. Lacerda, Samyra Maria Santos N.Nassif Brandão-Dias, Pedro Ferreira Pinto Kalapothakis, Evanguedes Silva Júnior, Alaion Fonseca da Alvarenga, Érika Ramos de França, Luiz Renato de Allotransplantation Aquaculture Blastula Catfish Cell Viability Controlled Study Developmental Stage Embryo Endangered Species Fertilization In Vitro Gametogenesis Genetic Analysis Genetic Marker Genetic Trait Germ Cell Germ Cell Transplantation Germplasm Heredity Laboratory Test Larva Male Nonhuman Oreochromis Niloticus Phylogeny Priority Journal Progeny Recipient Rhamdia Quelen Seminiferous Tubule Somatic Cell Spermatid Spermatocyte Spermatogenesis Spermatogonium Testis Development Tilapia Animals Cell Transplantation Cytology Environmental Protection Genetics Growth, Development And Aging Metabolism Physiology Procedures Spermatozoon Testis Xenograft Animal Aquaculture Catfishes Cell Transplantation Conservation Of Natural Resources Endangered Species Heterografts Male Seminiferous Tubules Spermatids Spermatocytes Spermatogenesis Spermatogonia Spermatozoa Testis Tilapia Transplantation, Heterologous Fish germ cell transplantation presents several important potential applications for aquaculture, including the preservation of germplasm from endangered fish species with high genetic and commercial values. Using this technique in studies developed in our laboratory with adult male Nile tilapias (Oreochromis niloticus), all the necessary procedures were successfully established, allowing the production of functional sperm and healthy progeny approximately 2 months after allogeneic transplantation. In the present study, we evaluated the viability of the adult Nile tilapia testis to generate sperm after xenogeneic transplant of germ cells from sexually mature Jundia catfish (Rhamdia quelen) that belong to a different taxonomic order. Therefore, in order to investigate at different time-periods post-transplantation, the presence and development of donor PKH26 labeled catfish germ cells were followed in the tilapia seminiferous tubules. From 7 to 20 days post-transplantation, only PKH26 labeled spermatogonia were observed, whereas spermatocytes at different stages of development were found at 70 days. Germ cell transplantation success and progression of spermatogenesis were indicated by the presence of labeled PKH26 spermatids and sperm on days 90 and 120 post-transplantation, respectively. Confirming the presence of the catfish genetic material in the tilapia testis, all recipient tilapias evaluated (n = 8) showed the genetic markers evaluated. Therefore, we demonstrated for the first time that the adult Nile tilapia testis offers the functional conditions for development of spermatogenesis with sperm production from a fish species belonging to a different order, which provides an important new venue for aquaculture advancement. © 2016 Elsevier Inc. 2020-05-19T13:43:49Z 2020-05-19T13:43:49Z 2016 Artigo https://repositorio.inpa.gov.br/handle/1/15816 10.1016/j.ygcen.2016.03.012 en Volume 230-231, Pags. 48-56 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf General and Comparative Endocrinology |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Allotransplantation Aquaculture Blastula Catfish Cell Viability Controlled Study Developmental Stage Embryo Endangered Species Fertilization In Vitro Gametogenesis Genetic Analysis Genetic Marker Genetic Trait Germ Cell Germ Cell Transplantation Germplasm Heredity Laboratory Test Larva Male Nonhuman Oreochromis Niloticus Phylogeny Priority Journal Progeny Recipient Rhamdia Quelen Seminiferous Tubule Somatic Cell Spermatid Spermatocyte Spermatogenesis Spermatogonium Testis Development Tilapia Animals Cell Transplantation Cytology Environmental Protection Genetics Growth, Development And Aging Metabolism Physiology Procedures Spermatozoon Testis Xenograft Animal Aquaculture Catfishes Cell Transplantation Conservation Of Natural Resources Endangered Species Heterografts Male Seminiferous Tubules Spermatids Spermatocytes Spermatogenesis Spermatogonia Spermatozoa Testis Tilapia Transplantation, Heterologous |
| spellingShingle |
Allotransplantation Aquaculture Blastula Catfish Cell Viability Controlled Study Developmental Stage Embryo Endangered Species Fertilization In Vitro Gametogenesis Genetic Analysis Genetic Marker Genetic Trait Germ Cell Germ Cell Transplantation Germplasm Heredity Laboratory Test Larva Male Nonhuman Oreochromis Niloticus Phylogeny Priority Journal Progeny Recipient Rhamdia Quelen Seminiferous Tubule Somatic Cell Spermatid Spermatocyte Spermatogenesis Spermatogonium Testis Development Tilapia Animals Cell Transplantation Cytology Environmental Protection Genetics Growth, Development And Aging Metabolism Physiology Procedures Spermatozoon Testis Xenograft Animal Aquaculture Catfishes Cell Transplantation Conservation Of Natural Resources Endangered Species Heterografts Male Seminiferous Tubules Spermatids Spermatocytes Spermatogenesis Spermatogonia Spermatozoa Testis Tilapia Transplantation, Heterologous Silva, Martinho de Almeida E. Successful xenogeneic germ cell transplantation from Jundia catfish (Rhamdia quelen) into adult Nile tilapia (Oreochromis niloticus) testes |
| topic_facet |
Allotransplantation Aquaculture Blastula Catfish Cell Viability Controlled Study Developmental Stage Embryo Endangered Species Fertilization In Vitro Gametogenesis Genetic Analysis Genetic Marker Genetic Trait Germ Cell Germ Cell Transplantation Germplasm Heredity Laboratory Test Larva Male Nonhuman Oreochromis Niloticus Phylogeny Priority Journal Progeny Recipient Rhamdia Quelen Seminiferous Tubule Somatic Cell Spermatid Spermatocyte Spermatogenesis Spermatogonium Testis Development Tilapia Animals Cell Transplantation Cytology Environmental Protection Genetics Growth, Development And Aging Metabolism Physiology Procedures Spermatozoon Testis Xenograft Animal Aquaculture Catfishes Cell Transplantation Conservation Of Natural Resources Endangered Species Heterografts Male Seminiferous Tubules Spermatids Spermatocytes Spermatogenesis Spermatogonia Spermatozoa Testis Tilapia Transplantation, Heterologous |
| description |
Fish germ cell transplantation presents several important potential applications for aquaculture, including the preservation of germplasm from endangered fish species with high genetic and commercial values. Using this technique in studies developed in our laboratory with adult male Nile tilapias (Oreochromis niloticus), all the necessary procedures were successfully established, allowing the production of functional sperm and healthy progeny approximately 2 months after allogeneic transplantation. In the present study, we evaluated the viability of the adult Nile tilapia testis to generate sperm after xenogeneic transplant of germ cells from sexually mature Jundia catfish (Rhamdia quelen) that belong to a different taxonomic order. Therefore, in order to investigate at different time-periods post-transplantation, the presence and development of donor PKH26 labeled catfish germ cells were followed in the tilapia seminiferous tubules. From 7 to 20 days post-transplantation, only PKH26 labeled spermatogonia were observed, whereas spermatocytes at different stages of development were found at 70 days. Germ cell transplantation success and progression of spermatogenesis were indicated by the presence of labeled PKH26 spermatids and sperm on days 90 and 120 post-transplantation, respectively. Confirming the presence of the catfish genetic material in the tilapia testis, all recipient tilapias evaluated (n = 8) showed the genetic markers evaluated. Therefore, we demonstrated for the first time that the adult Nile tilapia testis offers the functional conditions for development of spermatogenesis with sperm production from a fish species belonging to a different order, which provides an important new venue for aquaculture advancement. © 2016 Elsevier Inc. |
| format |
Artigo |
| author |
Silva, Martinho de Almeida E. |
| author2 |
Costa, Guilherme M.J. Lacerda, Samyra Maria Santos N.Nassif Brandão-Dias, Pedro Ferreira Pinto Kalapothakis, Evanguedes Silva Júnior, Alaion Fonseca da Alvarenga, Érika Ramos de França, Luiz Renato de |
| author2Str |
Costa, Guilherme M.J. Lacerda, Samyra Maria Santos N.Nassif Brandão-Dias, Pedro Ferreira Pinto Kalapothakis, Evanguedes Silva Júnior, Alaion Fonseca da Alvarenga, Érika Ramos de França, Luiz Renato de |
| title |
Successful xenogeneic germ cell transplantation from Jundia catfish (Rhamdia quelen) into adult Nile tilapia (Oreochromis niloticus) testes |
| title_short |
Successful xenogeneic germ cell transplantation from Jundia catfish (Rhamdia quelen) into adult Nile tilapia (Oreochromis niloticus) testes |
| title_full |
Successful xenogeneic germ cell transplantation from Jundia catfish (Rhamdia quelen) into adult Nile tilapia (Oreochromis niloticus) testes |
| title_fullStr |
Successful xenogeneic germ cell transplantation from Jundia catfish (Rhamdia quelen) into adult Nile tilapia (Oreochromis niloticus) testes |
| title_full_unstemmed |
Successful xenogeneic germ cell transplantation from Jundia catfish (Rhamdia quelen) into adult Nile tilapia (Oreochromis niloticus) testes |
| title_sort |
successful xenogeneic germ cell transplantation from jundia catfish (rhamdia quelen) into adult nile tilapia (oreochromis niloticus) testes |
| publisher |
General and Comparative Endocrinology |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/15816 |
| _version_ |
1787141764730060800 |
| score |
11.653393 |