A novel vasorelaxant lectin purified from seeds of Clathrotropis nitida: Partial characterization and immobilization in chitosan beads
A novel lectin from seeds of Clathrotropis nitida (CNA) was purified and characterized. CNA is a glycoprotein containing approximately 3.3% carbohydrates in its structure. CNA promoted intense agglutination of rabbit erythrocytes, which was inhibited by galactosides and porcine stomach mucin (PSM)....
| Autor principal: | Alves, Ana Cecília |
|---|---|
| Outros Autores: | Vasconcelos, Mayron Alves de, Santiago, Mayara Quiroz, Pinto-Junior, Vanir Reis, Osterne, Vinicius José Silva, Lóssio, Cláudia Figueiredo, Souza Ferreira Bringel, Pedro Henrique, Castro, Rondinelle Ribeiro, Nagano, C. S., Delatorre, Plínio, Souza, Luis Augusto Gomes, Nascimento, K. S., Assreuy, Ana Maria Sampaio, Cavada, B. S. |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Archives of Biochemistry and Biophysics
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/15873 |
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oai:repositorio:1-15873 |
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oai:repositorio:1-15873 A novel vasorelaxant lectin purified from seeds of Clathrotropis nitida: Partial characterization and immobilization in chitosan beads Alves, Ana Cecília Vasconcelos, Mayron Alves de Santiago, Mayara Quiroz Pinto-Junior, Vanir Reis Osterne, Vinicius José Silva Lóssio, Cláudia Figueiredo Souza Ferreira Bringel, Pedro Henrique Castro, Rondinelle Ribeiro Nagano, C. S. Delatorre, Plínio Souza, Luis Augusto Gomes Nascimento, K. S. Assreuy, Ana Maria Sampaio Cavada, B. S. Calcium Carbohydrate Chitosan Galactoside Lectin Magnesium Stomach Mucin Immobilized Protein Plant Lectin Vasodilator Agent Animals Cell Animals Experiment Animals Model Binding Affinity Carbohydrate Analysis Clathrotropis Nitida Controlled Study Dose Response Electrospray Mass Spectrometry Glycobiology Male Medicinal Plant Molecular Weight Nonhuman Physical Chemistry Seed Plant Electrophoresis, Polyacrylamide Gel Priority Journal Protein Analysis Protein Immobilization Rat Temperature Vascular Ring Vasodilatation Amino Acid Sequence Animals Artemia Chemistry Drug Effects Fabaceae Genetics Hemagglutination Human In Vitro Study Isolation And Purification Molecular Genetics Physiology Rabbit Sequence Homology Aorta, Thoracic Wistar Rat Amino Acid Sequence Animal Aorta, Thoracic Artemia Chitosan Fabaceae Hemagglutination Humans Immobilized Proteins In Vitro Techniques Male Molecular Sequence Data Molecular Weight Plant Lectins Plants, Medicinal Rabbits Rats Rats, Wistar Seeds Sequence Homology, Amino Acid Vasodilator Agents A novel lectin from seeds of Clathrotropis nitida (CNA) was purified and characterized. CNA is a glycoprotein containing approximately 3.3% carbohydrates in its structure. CNA promoted intense agglutination of rabbit erythrocytes, which was inhibited by galactosides and porcine stomach mucin (PSM). The lectin maintained its hemagglutinating activity after incubation in a wide range of temperatures (30-60 °C) and pH (6.0-7.0), and its binding activity was dependent on divalent cations (Ca+2 and Mg+2). SDS-PAGE showed an electrophoretic profile consisting of a single band of 28 kDa, as confirmed by electrospray ionization mass spectrometry, which indicated an average molecular mass of 27,406 ± 2 Da and the possible presence of isoforms and glycoforms. In addition, CNA exhibited no toxicity to Artemia sp. nauplii and elicited reversible and dose-dependent vasorelaxation in precontracted aortic rings. CNA was successfully immobilized on chitosan beads and was able to capture PSM in solution. This study demonstrated that CNA is a lectin that has potential as a biotechnological tool in glycomics and glycoproteomics applications. © 2015 Elsevier Inc. 2020-05-19T20:34:03Z 2020-05-19T20:34:03Z 2015 Artigo https://repositorio.inpa.gov.br/handle/1/15873 10.1016/j.abb.2015.10.020 en Volume 588, Pags. 33-40 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Archives of Biochemistry and Biophysics |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Calcium Carbohydrate Chitosan Galactoside Lectin Magnesium Stomach Mucin Immobilized Protein Plant Lectin Vasodilator Agent Animals Cell Animals Experiment Animals Model Binding Affinity Carbohydrate Analysis Clathrotropis Nitida Controlled Study Dose Response Electrospray Mass Spectrometry Glycobiology Male Medicinal Plant Molecular Weight Nonhuman Physical Chemistry Seed Plant Electrophoresis, Polyacrylamide Gel Priority Journal Protein Analysis Protein Immobilization Rat Temperature Vascular Ring Vasodilatation Amino Acid Sequence Animals Artemia Chemistry Drug Effects Fabaceae Genetics Hemagglutination Human In Vitro Study Isolation And Purification Molecular Genetics Physiology Rabbit Sequence Homology Aorta, Thoracic Wistar Rat Amino Acid Sequence Animal Aorta, Thoracic Artemia Chitosan Fabaceae Hemagglutination Humans Immobilized Proteins In Vitro Techniques Male Molecular Sequence Data Molecular Weight Plant Lectins Plants, Medicinal Rabbits Rats Rats, Wistar Seeds Sequence Homology, Amino Acid Vasodilator Agents |
| spellingShingle |
Calcium Carbohydrate Chitosan Galactoside Lectin Magnesium Stomach Mucin Immobilized Protein Plant Lectin Vasodilator Agent Animals Cell Animals Experiment Animals Model Binding Affinity Carbohydrate Analysis Clathrotropis Nitida Controlled Study Dose Response Electrospray Mass Spectrometry Glycobiology Male Medicinal Plant Molecular Weight Nonhuman Physical Chemistry Seed Plant Electrophoresis, Polyacrylamide Gel Priority Journal Protein Analysis Protein Immobilization Rat Temperature Vascular Ring Vasodilatation Amino Acid Sequence Animals Artemia Chemistry Drug Effects Fabaceae Genetics Hemagglutination Human In Vitro Study Isolation And Purification Molecular Genetics Physiology Rabbit Sequence Homology Aorta, Thoracic Wistar Rat Amino Acid Sequence Animal Aorta, Thoracic Artemia Chitosan Fabaceae Hemagglutination Humans Immobilized Proteins In Vitro Techniques Male Molecular Sequence Data Molecular Weight Plant Lectins Plants, Medicinal Rabbits Rats Rats, Wistar Seeds Sequence Homology, Amino Acid Vasodilator Agents Alves, Ana Cecília A novel vasorelaxant lectin purified from seeds of Clathrotropis nitida: Partial characterization and immobilization in chitosan beads |
| topic_facet |
Calcium Carbohydrate Chitosan Galactoside Lectin Magnesium Stomach Mucin Immobilized Protein Plant Lectin Vasodilator Agent Animals Cell Animals Experiment Animals Model Binding Affinity Carbohydrate Analysis Clathrotropis Nitida Controlled Study Dose Response Electrospray Mass Spectrometry Glycobiology Male Medicinal Plant Molecular Weight Nonhuman Physical Chemistry Seed Plant Electrophoresis, Polyacrylamide Gel Priority Journal Protein Analysis Protein Immobilization Rat Temperature Vascular Ring Vasodilatation Amino Acid Sequence Animals Artemia Chemistry Drug Effects Fabaceae Genetics Hemagglutination Human In Vitro Study Isolation And Purification Molecular Genetics Physiology Rabbit Sequence Homology Aorta, Thoracic Wistar Rat Amino Acid Sequence Animal Aorta, Thoracic Artemia Chitosan Fabaceae Hemagglutination Humans Immobilized Proteins In Vitro Techniques Male Molecular Sequence Data Molecular Weight Plant Lectins Plants, Medicinal Rabbits Rats Rats, Wistar Seeds Sequence Homology, Amino Acid Vasodilator Agents |
| description |
A novel lectin from seeds of Clathrotropis nitida (CNA) was purified and characterized. CNA is a glycoprotein containing approximately 3.3% carbohydrates in its structure. CNA promoted intense agglutination of rabbit erythrocytes, which was inhibited by galactosides and porcine stomach mucin (PSM). The lectin maintained its hemagglutinating activity after incubation in a wide range of temperatures (30-60 °C) and pH (6.0-7.0), and its binding activity was dependent on divalent cations (Ca+2 and Mg+2). SDS-PAGE showed an electrophoretic profile consisting of a single band of 28 kDa, as confirmed by electrospray ionization mass spectrometry, which indicated an average molecular mass of 27,406 ± 2 Da and the possible presence of isoforms and glycoforms. In addition, CNA exhibited no toxicity to Artemia sp. nauplii and elicited reversible and dose-dependent vasorelaxation in precontracted aortic rings. CNA was successfully immobilized on chitosan beads and was able to capture PSM in solution. This study demonstrated that CNA is a lectin that has potential as a biotechnological tool in glycomics and glycoproteomics applications. © 2015 Elsevier Inc. |
| format |
Artigo |
| author |
Alves, Ana Cecília |
| author2 |
Vasconcelos, Mayron Alves de Santiago, Mayara Quiroz Pinto-Junior, Vanir Reis Osterne, Vinicius José Silva Lóssio, Cláudia Figueiredo Souza Ferreira Bringel, Pedro Henrique Castro, Rondinelle Ribeiro Nagano, C. S. Delatorre, Plínio Souza, Luis Augusto Gomes Nascimento, K. S. Assreuy, Ana Maria Sampaio Cavada, B. S. |
| author2Str |
Vasconcelos, Mayron Alves de Santiago, Mayara Quiroz Pinto-Junior, Vanir Reis Osterne, Vinicius José Silva Lóssio, Cláudia Figueiredo Souza Ferreira Bringel, Pedro Henrique Castro, Rondinelle Ribeiro Nagano, C. S. Delatorre, Plínio Souza, Luis Augusto Gomes Nascimento, K. S. Assreuy, Ana Maria Sampaio Cavada, B. S. |
| title |
A novel vasorelaxant lectin purified from seeds of Clathrotropis nitida: Partial characterization and immobilization in chitosan beads |
| title_short |
A novel vasorelaxant lectin purified from seeds of Clathrotropis nitida: Partial characterization and immobilization in chitosan beads |
| title_full |
A novel vasorelaxant lectin purified from seeds of Clathrotropis nitida: Partial characterization and immobilization in chitosan beads |
| title_fullStr |
A novel vasorelaxant lectin purified from seeds of Clathrotropis nitida: Partial characterization and immobilization in chitosan beads |
| title_full_unstemmed |
A novel vasorelaxant lectin purified from seeds of Clathrotropis nitida: Partial characterization and immobilization in chitosan beads |
| title_sort |
novel vasorelaxant lectin purified from seeds of clathrotropis nitida: partial characterization and immobilization in chitosan beads |
| publisher |
Archives of Biochemistry and Biophysics |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/15873 |
| _version_ |
1787143548053749760 |
| score |
11.755432 |