Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds
A lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour-diffusion method using a precipitant solution consisting of 0.1 M Tris-HCl pH 7.8, 8%(w/v) PEG 3350 and 0.2 M proline at a constant temperature o...
| Autor principal: | Cavada, B. S. |
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| Outros Autores: | Marinho, Emmanuel S., Souza, Emmanuel Prata, Benevides, R. G., Delatorre, Plínio, Souza, Luis A.G., Nascimento, K. S., Sampaio, Alexandre Holanda, Moreno, Frederico Bruno Mendes Batista, Rustiguel, Joane Kathelen Rodrigues, Canduri, Fernanda, Azevedo, Walter Filgueira de, Debray, Henri |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Acta Crystallographica Section F: Structural Biology and Crystallization Communications
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/18792 |
| Resumo: |
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A lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour-diffusion method using a precipitant solution consisting of 0.1 M Tris-HCl pH 7.8, 8%(w/v) PEG 3350 and 0.2 M proline at a constant temperature of 293 K. A data set was collected to 1.77 Å resolution at a synchrotron-radiation source. CRL crystals are orthorhombic, belonging to space group P212121. Crystallographic refinement and full amino-acid sequence determination are in progress. © 2006 International Union of Crystallography. All rights reserved. |