Artigo

Screening of basidiomycetes from Amazonia for the production of biotechnological interest enzymes

Mushrooms, edible basidiomycetes, have been extensively used as producers of different substances of economical interest, such as enzymes, antibiotics, vitamins, amino acids, and steroids. The objective of this study is to detect the production of enzymes such as amylases, proteases, cellulases, phe...

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Autor principal: Souza, Helenires Queiroz de
Outros Autores: Oliveira, Luiz Antonio de, Andrade, Jerusa Souza
Grau: Artigo
Idioma: pt_BR
Publicado em: Ciência e Tecnologia de Alimentos 2020
Assuntos:
Basidiomycota
Cantharellus
Daedalea
Pycnoporus (fungus)
Pycnoporus Sanguineus
Stereaceae
Triticum Aestivum
Acesso em linha: https://repositorio.inpa.gov.br/handle/1/16319
id oai:repositorio:1-16319
recordtype dspace
spelling oai:repositorio:1-16319 Screening of basidiomycetes from Amazonia for the production of biotechnological interest enzymes Seleção de Basidiomycetes da Amazônia para produção de enzimas de interesse biotecnológico Souza, Helenires Queiroz de Oliveira, Luiz Antonio de Andrade, Jerusa Souza Basidiomycota Cantharellus Daedalea Pycnoporus (fungus) Pycnoporus Sanguineus Stereaceae Triticum Aestivum Mushrooms, edible basidiomycetes, have been extensively used as producers of different substances of economical interest, such as enzymes, antibiotics, vitamins, amino acids, and steroids. The objective of this study is to detect the production of enzymes such as amylases, proteases, cellulases, phenoloxidases and pectinases for lineages of basidiomycetes originating from the Amazonian forest. For the production of enzymes, mushrooms were cultivated in a liquid medium with substratum (0,5%), pH adjusted for each enzyme, and incubated at 28 °C, under agitation and 140 rpm for 96 or 120 hours. After this period, the samples were filtered for the separation of the mycelial mass. The filtrates were inoculated in solid medium surface perforated cup-plates of 6 mm diameter, appropriate for the detection of enzymes in Petri dishes. The plates were incubated at 28 °C for 24 hours, for observation to reveal enzymatic halos. It was verified the activity of the amylase and protease produced by the mushrooms grown in the liquid medium, with different nutritive sources. It was possible to detect the cellulase and protease production for all the ones that were isolated, 40% produced amylases, 50% produced phenoloxidases, and 10% produced pectinases. Regarding the activity of the amylase, the wheat bran substrate presented the largest degradation halos revealing Daedalea sp.4E6 and Daedalea sp. 1A, Stereaceae 22B and Pycnoporus sanguineus 12B mushrooms. Considering the substrata tested for protease production, it was observed that the fish protein concentrate was the best. The P. sanguineus 12B, Stereaceae 22B and Cantharellus guyanensis 4Bl mushrooms were the best protease producers. 2020-06-03T19:27:22Z 2020-06-03T19:27:22Z 2008 Artigo https://repositorio.inpa.gov.br/handle/1/16319 10.1590/S0101-20612008000500019 pt_BR Volume 28, Número SUPPL., Pags. 116-124 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Ciência e Tecnologia de Alimentos
institution Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional
collection INPA-RI
language pt_BR
topic Basidiomycota
Cantharellus
Daedalea
Pycnoporus (fungus)
Pycnoporus Sanguineus
Stereaceae
Triticum Aestivum
spellingShingle Basidiomycota
Cantharellus
Daedalea
Pycnoporus (fungus)
Pycnoporus Sanguineus
Stereaceae
Triticum Aestivum
Souza, Helenires Queiroz de
Screening of basidiomycetes from Amazonia for the production of biotechnological interest enzymes
topic_facet Basidiomycota
Cantharellus
Daedalea
Pycnoporus (fungus)
Pycnoporus Sanguineus
Stereaceae
Triticum Aestivum
description Mushrooms, edible basidiomycetes, have been extensively used as producers of different substances of economical interest, such as enzymes, antibiotics, vitamins, amino acids, and steroids. The objective of this study is to detect the production of enzymes such as amylases, proteases, cellulases, phenoloxidases and pectinases for lineages of basidiomycetes originating from the Amazonian forest. For the production of enzymes, mushrooms were cultivated in a liquid medium with substratum (0,5%), pH adjusted for each enzyme, and incubated at 28 °C, under agitation and 140 rpm for 96 or 120 hours. After this period, the samples were filtered for the separation of the mycelial mass. The filtrates were inoculated in solid medium surface perforated cup-plates of 6 mm diameter, appropriate for the detection of enzymes in Petri dishes. The plates were incubated at 28 °C for 24 hours, for observation to reveal enzymatic halos. It was verified the activity of the amylase and protease produced by the mushrooms grown in the liquid medium, with different nutritive sources. It was possible to detect the cellulase and protease production for all the ones that were isolated, 40% produced amylases, 50% produced phenoloxidases, and 10% produced pectinases. Regarding the activity of the amylase, the wheat bran substrate presented the largest degradation halos revealing Daedalea sp.4E6 and Daedalea sp. 1A, Stereaceae 22B and Pycnoporus sanguineus 12B mushrooms. Considering the substrata tested for protease production, it was observed that the fish protein concentrate was the best. The P. sanguineus 12B, Stereaceae 22B and Cantharellus guyanensis 4Bl mushrooms were the best protease producers.
format Artigo
author Souza, Helenires Queiroz de
author2 Oliveira, Luiz Antonio de
Andrade, Jerusa Souza
author2Str Oliveira, Luiz Antonio de
Andrade, Jerusa Souza
title Screening of basidiomycetes from Amazonia for the production of biotechnological interest enzymes
title_short Screening of basidiomycetes from Amazonia for the production of biotechnological interest enzymes
title_full Screening of basidiomycetes from Amazonia for the production of biotechnological interest enzymes
title_fullStr Screening of basidiomycetes from Amazonia for the production of biotechnological interest enzymes
title_full_unstemmed Screening of basidiomycetes from Amazonia for the production of biotechnological interest enzymes
title_sort screening of basidiomycetes from amazonia for the production of biotechnological interest enzymes
publisher Ciência e Tecnologia de Alimentos
publishDate 2020
url https://repositorio.inpa.gov.br/handle/1/16319
_version_ 1787144542366990336
score 11.653393

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