Isolation and characterisation of novel polymorphic microsatellite markers for the Wedge-billed Woodcreeper Glyphorynchus spirurus
In order to study the population genetic structure of the Amazonian understorey bird Glyphorynchus spirurus, we have isolated eight polymorphic microsatellite loci using next-generation sequencing. All loci were screened using 40 samples from central Amazonia. The number of alleles varied from five...
| Autor principal: | Unrein, Jasmin |
|---|---|
| Outros Autores: | Menger, Juliana, Weigert, Anne, Henle, Klaus, Schlegel, Martin |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Avian Biology Research
2020
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| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/17169 |
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oai:repositorio:1-17169 |
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oai:repositorio:1-17169 Isolation and characterisation of novel polymorphic microsatellite markers for the Wedge-billed Woodcreeper Glyphorynchus spirurus Unrein, Jasmin Menger, Juliana Weigert, Anne Henle, Klaus Schlegel, Martin Allele Bird Clinical Gene Linkage Disequilibrium Heterozygosity Human Microsatellite Marker Neotropics Next Generation Sequencing Nonhuman Population Genetic Structure Understorey In order to study the population genetic structure of the Amazonian understorey bird Glyphorynchus spirurus, we have isolated eight polymorphic microsatellite loci using next-generation sequencing. All loci were screened using 40 samples from central Amazonia. The number of alleles varied from five to 22. The observed heterozygosities ranged from 0.475 to 0.875. No linkage disequilibrium was found among the loci. None of the loci showed significant deviation from Hardy-Weinberg equilibrium. These novel microsatellite markers will be a useful tool for future population genetics and ecological studies of the Wedge-billed Woodcreeper. 2020-06-15T21:39:36Z 2020-06-15T21:39:36Z 2017 Artigo https://repositorio.inpa.gov.br/handle/1/17169 10.3184/175815617X14799886573066 en Volume 10, Número 1, Pags. 24-26 Restrito Avian Biology Research |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Allele Bird Clinical Gene Linkage Disequilibrium Heterozygosity Human Microsatellite Marker Neotropics Next Generation Sequencing Nonhuman Population Genetic Structure Understorey |
| spellingShingle |
Allele Bird Clinical Gene Linkage Disequilibrium Heterozygosity Human Microsatellite Marker Neotropics Next Generation Sequencing Nonhuman Population Genetic Structure Understorey Unrein, Jasmin Isolation and characterisation of novel polymorphic microsatellite markers for the Wedge-billed Woodcreeper Glyphorynchus spirurus |
| topic_facet |
Allele Bird Clinical Gene Linkage Disequilibrium Heterozygosity Human Microsatellite Marker Neotropics Next Generation Sequencing Nonhuman Population Genetic Structure Understorey |
| description |
In order to study the population genetic structure of the Amazonian understorey bird Glyphorynchus spirurus, we have isolated eight polymorphic microsatellite loci using next-generation sequencing. All loci were screened using 40 samples from central Amazonia. The number of alleles varied from five to 22. The observed heterozygosities ranged from 0.475 to 0.875. No linkage disequilibrium was found among the loci. None of the loci showed significant deviation from Hardy-Weinberg equilibrium. These novel microsatellite markers will be a useful tool for future population genetics and ecological studies of the Wedge-billed Woodcreeper. |
| format |
Artigo |
| author |
Unrein, Jasmin |
| author2 |
Menger, Juliana Weigert, Anne Henle, Klaus Schlegel, Martin |
| author2Str |
Menger, Juliana Weigert, Anne Henle, Klaus Schlegel, Martin |
| title |
Isolation and characterisation of novel polymorphic microsatellite markers for the Wedge-billed Woodcreeper Glyphorynchus spirurus |
| title_short |
Isolation and characterisation of novel polymorphic microsatellite markers for the Wedge-billed Woodcreeper Glyphorynchus spirurus |
| title_full |
Isolation and characterisation of novel polymorphic microsatellite markers for the Wedge-billed Woodcreeper Glyphorynchus spirurus |
| title_fullStr |
Isolation and characterisation of novel polymorphic microsatellite markers for the Wedge-billed Woodcreeper Glyphorynchus spirurus |
| title_full_unstemmed |
Isolation and characterisation of novel polymorphic microsatellite markers for the Wedge-billed Woodcreeper Glyphorynchus spirurus |
| title_sort |
isolation and characterisation of novel polymorphic microsatellite markers for the wedge-billed woodcreeper glyphorynchus spirurus |
| publisher |
Avian Biology Research |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/17169 |
| _version_ |
1787141482948329472 |
| score |
11.653393 |