Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species
Anopheles (Nyssorhynchus) triannulatus is a complex of 3 species. Thirteen polymorphic microsatellite loci were isolated and characterized in 20 to 25 individuals from Manaus (AM, Brazil). The number of alleles per locus varied from 3 to 10 (mean = 6.0). The observed heterozygosity ranged from 0.250...
| Autor principal: | Cruz-Hernández, Pedro |
|---|---|
| Outros Autores: | Batista, Jacqueline da Silva, Formiga, Kyara M., Rafael, Míriam Silva, Tadei, Wanderli Pedro, Santos, Joselita Maria M. |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Genetics and Molecular Research
2020
|
| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/14993 |
| Resumo: |
|---|
|
Anopheles (Nyssorhynchus) triannulatus is a complex of 3 species. Thirteen polymorphic microsatellite loci were isolated and characterized in 20 to 25 individuals from Manaus (AM, Brazil). The number of alleles per locus varied from 3 to 10 (mean = 6.0). The observed heterozygosity ranged from 0.250 to 0.875 (mean = 0.680) and expected heterozygosity ranged from 0.376 to 0.844 (mean = 0.698). Two loci exhibited null alleles and all loci were in Hardy-Weinberg equilibrium. No linkage disequilibrium between loci was observed. These loci were used in 4 congeneric species and provide a useful tool for studying population genetics and other aspects of the biology of this and other Anopheles species. ©FUNPEC-RP. |