Evolutionary Dynamics of 5S rDNA and Recurrent Association of Transposable Elements in Electric Fish of the Family Gymnotidae (Gymnotiformes): The Case of Gymnotus mamiraua
Gymnotidae is a family of electric fish endemic to the Neotropics consisting of 2 genera: Electrophorus and Gymnotus. The genus Gymnotus is widely distributed and is found in all of the major Brazilian river systems. Physical and molecular mapping data for the ribosomal DNA (rDNA) in this genus are...
| Autor principal: | Silva, M. R. da |
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| Outros Autores: | Barbosa, Patrícia, Artoni, Roberto Ferreira, Feldberg, Eliana |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Cytogenetic and Genome Research
2020
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| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/17222 |
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oai:repositorio:1-17222 |
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oai:repositorio:1-17222 Evolutionary Dynamics of 5S rDNA and Recurrent Association of Transposable Elements in Electric Fish of the Family Gymnotidae (Gymnotiformes): The Case of Gymnotus mamiraua Silva, M. R. da Barbosa, Patrícia Artoni, Roberto Ferreira Feldberg, Eliana Dna 5s Retroposon Ribosome Dna Rna 5s Chromosomal Mapping Dna Isolation Dna Sequence Dna Synthesis Fish Genetics In Situ Hybridization, Fluorescence Gymnotiformes Gymnotus Mamiraua Cloning, Molecular Evolution, Molecular Nonhuman Priority Journal Retroposon Sequence Analysis Transposon Animals Female Genetics Genome Gymnotiformes Male Animal Chromosome Mapping Ribosomal Dna Evolution, Molecular Female Genome Gymnotiformes In Situ Hybridization, Fluorescence Male Retroelements Rna, Ribosomal, 5s Gymnotidae is a family of electric fish endemic to the Neotropics consisting of 2 genera: Electrophorus and Gymnotus. The genus Gymnotus is widely distributed and is found in all of the major Brazilian river systems. Physical and molecular mapping data for the ribosomal DNA (rDNA) in this genus are still scarce, with its chromosomal location known in only 11 species. As other species of Gymnotus with 2n = 54 chromosomes from the Paraná-Paraguay basin, G. mamiraua was found to have a large number of 5S rDNA sites. Isolation and cloning of the 5S rDNA sequences from G. mamiraua identified a fragment of a transposable element similar to the Tc1/mariner transposon associated with a non-transcribed spacer. Double fluorescence in situ hybridization analysis of this element and the 5S rDNA showed that they were colocalized on several chromosomes, in addition to acting as nonsyntenic markers on others. Our data show the association between these sequences and suggest that the Tc1 retrotransposon may be the agent that drives the spread of these 5S rDNA-like sequences in the G. mamiraua genome. © 2016 S. Karger AG, Basel. 2020-06-15T21:40:06Z 2020-06-15T21:40:06Z 2016 Artigo https://repositorio.inpa.gov.br/handle/1/17222 10.1159/000449431 en Volume 149, Número 4, Pags. 297-303 Restrito Cytogenetic and Genome Research |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Dna 5s Retroposon Ribosome Dna Rna 5s Chromosomal Mapping Dna Isolation Dna Sequence Dna Synthesis Fish Genetics In Situ Hybridization, Fluorescence Gymnotiformes Gymnotus Mamiraua Cloning, Molecular Evolution, Molecular Nonhuman Priority Journal Retroposon Sequence Analysis Transposon Animals Female Genetics Genome Gymnotiformes Male Animal Chromosome Mapping Ribosomal Dna Evolution, Molecular Female Genome Gymnotiformes In Situ Hybridization, Fluorescence Male Retroelements Rna, Ribosomal, 5s |
| spellingShingle |
Dna 5s Retroposon Ribosome Dna Rna 5s Chromosomal Mapping Dna Isolation Dna Sequence Dna Synthesis Fish Genetics In Situ Hybridization, Fluorescence Gymnotiformes Gymnotus Mamiraua Cloning, Molecular Evolution, Molecular Nonhuman Priority Journal Retroposon Sequence Analysis Transposon Animals Female Genetics Genome Gymnotiformes Male Animal Chromosome Mapping Ribosomal Dna Evolution, Molecular Female Genome Gymnotiformes In Situ Hybridization, Fluorescence Male Retroelements Rna, Ribosomal, 5s Silva, M. R. da Evolutionary Dynamics of 5S rDNA and Recurrent Association of Transposable Elements in Electric Fish of the Family Gymnotidae (Gymnotiformes): The Case of Gymnotus mamiraua |
| topic_facet |
Dna 5s Retroposon Ribosome Dna Rna 5s Chromosomal Mapping Dna Isolation Dna Sequence Dna Synthesis Fish Genetics In Situ Hybridization, Fluorescence Gymnotiformes Gymnotus Mamiraua Cloning, Molecular Evolution, Molecular Nonhuman Priority Journal Retroposon Sequence Analysis Transposon Animals Female Genetics Genome Gymnotiformes Male Animal Chromosome Mapping Ribosomal Dna Evolution, Molecular Female Genome Gymnotiformes In Situ Hybridization, Fluorescence Male Retroelements Rna, Ribosomal, 5s |
| description |
Gymnotidae is a family of electric fish endemic to the Neotropics consisting of 2 genera: Electrophorus and Gymnotus. The genus Gymnotus is widely distributed and is found in all of the major Brazilian river systems. Physical and molecular mapping data for the ribosomal DNA (rDNA) in this genus are still scarce, with its chromosomal location known in only 11 species. As other species of Gymnotus with 2n = 54 chromosomes from the Paraná-Paraguay basin, G. mamiraua was found to have a large number of 5S rDNA sites. Isolation and cloning of the 5S rDNA sequences from G. mamiraua identified a fragment of a transposable element similar to the Tc1/mariner transposon associated with a non-transcribed spacer. Double fluorescence in situ hybridization analysis of this element and the 5S rDNA showed that they were colocalized on several chromosomes, in addition to acting as nonsyntenic markers on others. Our data show the association between these sequences and suggest that the Tc1 retrotransposon may be the agent that drives the spread of these 5S rDNA-like sequences in the G. mamiraua genome. © 2016 S. Karger AG, Basel. |
| format |
Artigo |
| author |
Silva, M. R. da |
| author2 |
Barbosa, Patrícia Artoni, Roberto Ferreira Feldberg, Eliana |
| author2Str |
Barbosa, Patrícia Artoni, Roberto Ferreira Feldberg, Eliana |
| title |
Evolutionary Dynamics of 5S rDNA and Recurrent Association of Transposable Elements in Electric Fish of the Family Gymnotidae (Gymnotiformes): The Case of Gymnotus mamiraua |
| title_short |
Evolutionary Dynamics of 5S rDNA and Recurrent Association of Transposable Elements in Electric Fish of the Family Gymnotidae (Gymnotiformes): The Case of Gymnotus mamiraua |
| title_full |
Evolutionary Dynamics of 5S rDNA and Recurrent Association of Transposable Elements in Electric Fish of the Family Gymnotidae (Gymnotiformes): The Case of Gymnotus mamiraua |
| title_fullStr |
Evolutionary Dynamics of 5S rDNA and Recurrent Association of Transposable Elements in Electric Fish of the Family Gymnotidae (Gymnotiformes): The Case of Gymnotus mamiraua |
| title_full_unstemmed |
Evolutionary Dynamics of 5S rDNA and Recurrent Association of Transposable Elements in Electric Fish of the Family Gymnotidae (Gymnotiformes): The Case of Gymnotus mamiraua |
| title_sort |
evolutionary dynamics of 5s rdna and recurrent association of transposable elements in electric fish of the family gymnotidae (gymnotiformes): the case of gymnotus mamiraua |
| publisher |
Cytogenetic and Genome Research |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/17222 |
| _version_ |
1787142762912546816 |
| score |
11.674752 |