Artigo

Efficient and safe gene transfection in fish spermatogonial stem cells using nanomaterials

Multiwalled carbon nanotubes (MWCNTs), nanographene oxide (NGO), and gold nanorods (NRs) can be functionalized and complexed to DNA to promote efficient gene delivery to Nile tilapia spermatogonial stem cells inducing less cell death than electroporation and the commercial reagents tested. Therefore...

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Autor principal: Tonelli, Fernanda MP
Outros Autores: Lacerda, Samyra Maria Santos N.Nassif, Paiva, N. C O, Lemos, Moline Severino, Jesus, A. C. de, Pacheco, Flávia G., Corrêa-Junior, José D., Ladeira, Luiz Orlando, Furtado, Clascídia Aparecida, França, Luiz Renato de, Resende, Rodrigo Ribeiro
Grau: Artigo
Idioma: English
Publicado em: RSC Advances 2020
Assuntos:
Carbon
Cell Death
Fish
Gene Transfer
Genes
Multiwalled Carbon Nanotubes (mwcn)
Nanorods
Nanostructured Materials
Stem Cells
Yarn
Commercial Reagents
Electroporation
Functionalized
Gene Delivery
Gene Transfection
Gold Nanorod
Multiwalled Carbon Nanotube (mwcnts)
Nile Tilapia
Molecular Biology
Acesso em linha: https://repositorio.inpa.gov.br/handle/1/17412
Resumo:
Multiwalled carbon nanotubes (MWCNTs), nanographene oxide (NGO), and gold nanorods (NRs) can be functionalized and complexed to DNA to promote efficient gene delivery to Nile tilapia spermatogonial stem cells inducing less cell death than electroporation and the commercial reagents tested. Therefore, nanomaterials can contribute to achieve fish transgenesis. © The Royal Society of Chemistry.

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