Differentiation of two morphologically similar Amazonian Aniba species by mass spectrometry leaf fingerprinting
The wood of the Amazonian tree Aniba rosaeodora Ducke has been intensively exploited since the 1920s and remains a much valued material in the perfume industry, leading to its classification as a plant at risk of extinction. Another Amazonian tree, Aniba parviflora, is morphologically highly similar...
| Autor principal: | Galaverna, Renan de S. |
|---|---|
| Outros Autores: | Sampaio, Paulo de Tarso Barbosa, Barata, Lauro Euclides Soares, Eberlin, M. N., Fidélis, Carlos Henrique de Vasconcelos |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Analytical Methods
2020
|
| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/17490 |
| id |
oai:repositorio:1-17490 |
|---|---|
| recordtype |
dspace |
| spelling |
oai:repositorio:1-17490 Differentiation of two morphologically similar Amazonian Aniba species by mass spectrometry leaf fingerprinting Galaverna, Renan de S. Sampaio, Paulo de Tarso Barbosa Barata, Lauro Euclides Soares Eberlin, M. N. Fidélis, Carlos Henrique de Vasconcelos Drug Products Forestry Mass Spectrometry Plants (botany) Spectrometry Accurate Mass Measurements Illegal Practices Ionization Techniques Leaf Surfaces Mass Spectra Ms/ms Fragmentation Plant Species Reliable Methods Principal Component Analysis Mass Spectrometers Plants Wood The wood of the Amazonian tree Aniba rosaeodora Ducke has been intensively exploited since the 1920s and remains a much valued material in the perfume industry, leading to its classification as a plant at risk of extinction. Another Amazonian tree, Aniba parviflora, is morphologically highly similar to the much more valuable A. rosaeodora and is illegally commercialized in the seedling stage as counterfeit samples of the much more valuable A. rosaeodora. A technique that would be able to perform rapid, versatile and reliable differentiation of the two species would therefore be valuable to detect such illegal practice. Herein we describe unequivocal differentiation between the two morphologically similar Amazonian Aniba species by leaf mass spectrometry fingerprinting using a small fragment of the leaf. Two ionization techniques were tested (ESI and VL-EASI), and mass spectra obtained from many leaves and at different points on the leaf surface belonging to the same species were very similar and reproducible, whereas spectra between the two species were markedly different. Marker ions detected in each spectrum were structurally characterized based on the accurate mass measurements, MS/MS fragmentation patterns and comparison of the results with reported data. Principal component analysis (PCA) was used to validate the differentiation of the spectra. MS leaf fingerprinting using both ESI and VL-EASI offers therefore a rapid, versatile and reliable method to differentiate morphologically similar plant species, as demonstrated herein for the two Amazonian Aniba species at the seedling stage. This journal is © The Royal Society of Chemistry. 2020-06-15T21:48:04Z 2020-06-15T21:48:04Z 2015 Artigo https://repositorio.inpa.gov.br/handle/1/17490 10.1039/c4ay02598a en Volume 7, Número 5, Pags. 1984-1990 Restrito Analytical Methods |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Drug Products Forestry Mass Spectrometry Plants (botany) Spectrometry Accurate Mass Measurements Illegal Practices Ionization Techniques Leaf Surfaces Mass Spectra Ms/ms Fragmentation Plant Species Reliable Methods Principal Component Analysis Mass Spectrometers Plants Wood |
| spellingShingle |
Drug Products Forestry Mass Spectrometry Plants (botany) Spectrometry Accurate Mass Measurements Illegal Practices Ionization Techniques Leaf Surfaces Mass Spectra Ms/ms Fragmentation Plant Species Reliable Methods Principal Component Analysis Mass Spectrometers Plants Wood Galaverna, Renan de S. Differentiation of two morphologically similar Amazonian Aniba species by mass spectrometry leaf fingerprinting |
| topic_facet |
Drug Products Forestry Mass Spectrometry Plants (botany) Spectrometry Accurate Mass Measurements Illegal Practices Ionization Techniques Leaf Surfaces Mass Spectra Ms/ms Fragmentation Plant Species Reliable Methods Principal Component Analysis Mass Spectrometers Plants Wood |
| description |
The wood of the Amazonian tree Aniba rosaeodora Ducke has been intensively exploited since the 1920s and remains a much valued material in the perfume industry, leading to its classification as a plant at risk of extinction. Another Amazonian tree, Aniba parviflora, is morphologically highly similar to the much more valuable A. rosaeodora and is illegally commercialized in the seedling stage as counterfeit samples of the much more valuable A. rosaeodora. A technique that would be able to perform rapid, versatile and reliable differentiation of the two species would therefore be valuable to detect such illegal practice. Herein we describe unequivocal differentiation between the two morphologically similar Amazonian Aniba species by leaf mass spectrometry fingerprinting using a small fragment of the leaf. Two ionization techniques were tested (ESI and VL-EASI), and mass spectra obtained from many leaves and at different points on the leaf surface belonging to the same species were very similar and reproducible, whereas spectra between the two species were markedly different. Marker ions detected in each spectrum were structurally characterized based on the accurate mass measurements, MS/MS fragmentation patterns and comparison of the results with reported data. Principal component analysis (PCA) was used to validate the differentiation of the spectra. MS leaf fingerprinting using both ESI and VL-EASI offers therefore a rapid, versatile and reliable method to differentiate morphologically similar plant species, as demonstrated herein for the two Amazonian Aniba species at the seedling stage. This journal is © The Royal Society of Chemistry. |
| format |
Artigo |
| author |
Galaverna, Renan de S. |
| author2 |
Sampaio, Paulo de Tarso Barbosa Barata, Lauro Euclides Soares Eberlin, M. N. Fidélis, Carlos Henrique de Vasconcelos |
| author2Str |
Sampaio, Paulo de Tarso Barbosa Barata, Lauro Euclides Soares Eberlin, M. N. Fidélis, Carlos Henrique de Vasconcelos |
| title |
Differentiation of two morphologically similar Amazonian Aniba species by mass spectrometry leaf fingerprinting |
| title_short |
Differentiation of two morphologically similar Amazonian Aniba species by mass spectrometry leaf fingerprinting |
| title_full |
Differentiation of two morphologically similar Amazonian Aniba species by mass spectrometry leaf fingerprinting |
| title_fullStr |
Differentiation of two morphologically similar Amazonian Aniba species by mass spectrometry leaf fingerprinting |
| title_full_unstemmed |
Differentiation of two morphologically similar Amazonian Aniba species by mass spectrometry leaf fingerprinting |
| title_sort |
differentiation of two morphologically similar amazonian aniba species by mass spectrometry leaf fingerprinting |
| publisher |
Analytical Methods |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/17490 |
| _version_ |
1787145271894867968 |
| score |
11.755432 |