The influence of a TNF gene polymorphism on the severity of rheumatoid arthritis in the Brazilian Amazon
Purpose: The aim of this study was to investigate the influence of the TNF -308 G/A polymorphism in the promoter region of the tumor necrosis factor- α gene on the susceptibility and severity of rheumatoid arthritis (RA) in individuals from the Brazilian Amazon. Methods: A total of 323 individuals-1...
| Autor principal: | Boechat, Antonio Luiz |
|---|---|
| Outros Autores: | Boechat, Narjara de Oliveira, Ogusku, Maurício Morishi, Alencar, Mariana Raposo, Abensur, Themis da Costa, Neto, José Cardoso, Amorim, Leilian de Souza, Oliveira, Lívia Moura de, Sadahiro, Aya, Santos, Maria Cristina dos |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Cytokine
2020
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| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/17912 |
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oai:repositorio:1-17912 |
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oai:repositorio:1-17912 The influence of a TNF gene polymorphism on the severity of rheumatoid arthritis in the Brazilian Amazon Boechat, Antonio Luiz Boechat, Narjara de Oliveira Ogusku, Maurício Morishi Alencar, Mariana Raposo Abensur, Themis da Costa Neto, José Cardoso Amorim, Leilian de Souza Oliveira, Lívia Moura de Sadahiro, Aya Santos, Maria Cristina dos Disease Modifying Antirheumatic Drug Dna Rheumatoid Factor Tumor Necrosis Factor-alpha Tumor Necrosis Factor Alpha Inhibitor Adult Age Aged Brasil Clinical Feature Confidence Interval Controlled Study Das28 Disease Duration Disease Severity Dna Extraction Female Gene Frequency Polymorphism, Genetic Genetic Susceptibility Genotype Health Assessment Questionnaire Human Logistic Regression Analysis Major Clinical Study Male Polymerase Chain Reaction Priority Journal Promoter Region Polymorphism, Restriction Fragment Length Arthritis, Rheumatoid Risk Assessment Tnf Gene Adolescent Adult Aged Alleles Arthritis, Rheumatoid Brasil Case-control Studies Demography Female Genetic Predisposition To Disease Humans Joints Logistic Models Male Middle Aged Models, Genetic Polymorphism, Single Nucleotide Reproducibility Of Results Roc Curve Tumor Necrosis Factor-alpha Young Adult Purpose: The aim of this study was to investigate the influence of the TNF -308 G/A polymorphism in the promoter region of the tumor necrosis factor- α gene on the susceptibility and severity of rheumatoid arthritis (RA) in individuals from the Brazilian Amazon. Methods: A total of 323 individuals-192 healthy controls without arthritis and 131 individuals suffering from arthritis-were genotyped for this polymorphism using a methodology based on PCR-RFLP. Results: The frequency of the A allele (TNF2) in rheumatoid arthritis sufferers was not significantly higher than in the controls (p= 0.926; OR = 0.97; confidence interval 0.54-1.76). However, using a logistic regression model, when the patients were stratified according to whether the manifestations were preponderantly articular or systemic, there was a strong association between the TNF2 allele and systemic arthritis (p= 0.001; OR = 5.89; confidence interval = 1.98-17.5) as well as the use of anti-TNF immunotherapy (p= 0.023; OR = 1.10; confidence interval = 1.00-1.14). The main factors that were found to influence the risk of extra-articular disease were age greater than or equal to 60. years (p= 0.008; OR = 4.06; confidence interval = 1.45-11.38), disease duration greater than 10. years (p= 0.031; OR = 3.10; confidence interval = 1.11-8.63) and positive rheumatoid factor (p= 0.035; OR = 2.07; confidence interval = 1.05-4.09). Conclusions: These results suggest that the TNF2 allele is associated with the more serious forms of the disease in individuals from the Brazilian Amazon but not with a risk for developing RA. © 2012 Elsevier Ltd. 2020-06-15T21:50:04Z 2020-06-15T21:50:04Z 2013 Artigo https://repositorio.inpa.gov.br/handle/1/17912 10.1016/j.cyto.2012.11.020 en Volume 61, Número 2, Pags. 406-412 Restrito Cytokine |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Disease Modifying Antirheumatic Drug Dna Rheumatoid Factor Tumor Necrosis Factor-alpha Tumor Necrosis Factor Alpha Inhibitor Adult Age Aged Brasil Clinical Feature Confidence Interval Controlled Study Das28 Disease Duration Disease Severity Dna Extraction Female Gene Frequency Polymorphism, Genetic Genetic Susceptibility Genotype Health Assessment Questionnaire Human Logistic Regression Analysis Major Clinical Study Male Polymerase Chain Reaction Priority Journal Promoter Region Polymorphism, Restriction Fragment Length Arthritis, Rheumatoid Risk Assessment Tnf Gene Adolescent Adult Aged Alleles Arthritis, Rheumatoid Brasil Case-control Studies Demography Female Genetic Predisposition To Disease Humans Joints Logistic Models Male Middle Aged Models, Genetic Polymorphism, Single Nucleotide Reproducibility Of Results Roc Curve Tumor Necrosis Factor-alpha Young Adult |
| spellingShingle |
Disease Modifying Antirheumatic Drug Dna Rheumatoid Factor Tumor Necrosis Factor-alpha Tumor Necrosis Factor Alpha Inhibitor Adult Age Aged Brasil Clinical Feature Confidence Interval Controlled Study Das28 Disease Duration Disease Severity Dna Extraction Female Gene Frequency Polymorphism, Genetic Genetic Susceptibility Genotype Health Assessment Questionnaire Human Logistic Regression Analysis Major Clinical Study Male Polymerase Chain Reaction Priority Journal Promoter Region Polymorphism, Restriction Fragment Length Arthritis, Rheumatoid Risk Assessment Tnf Gene Adolescent Adult Aged Alleles Arthritis, Rheumatoid Brasil Case-control Studies Demography Female Genetic Predisposition To Disease Humans Joints Logistic Models Male Middle Aged Models, Genetic Polymorphism, Single Nucleotide Reproducibility Of Results Roc Curve Tumor Necrosis Factor-alpha Young Adult Boechat, Antonio Luiz The influence of a TNF gene polymorphism on the severity of rheumatoid arthritis in the Brazilian Amazon |
| topic_facet |
Disease Modifying Antirheumatic Drug Dna Rheumatoid Factor Tumor Necrosis Factor-alpha Tumor Necrosis Factor Alpha Inhibitor Adult Age Aged Brasil Clinical Feature Confidence Interval Controlled Study Das28 Disease Duration Disease Severity Dna Extraction Female Gene Frequency Polymorphism, Genetic Genetic Susceptibility Genotype Health Assessment Questionnaire Human Logistic Regression Analysis Major Clinical Study Male Polymerase Chain Reaction Priority Journal Promoter Region Polymorphism, Restriction Fragment Length Arthritis, Rheumatoid Risk Assessment Tnf Gene Adolescent Adult Aged Alleles Arthritis, Rheumatoid Brasil Case-control Studies Demography Female Genetic Predisposition To Disease Humans Joints Logistic Models Male Middle Aged Models, Genetic Polymorphism, Single Nucleotide Reproducibility Of Results Roc Curve Tumor Necrosis Factor-alpha Young Adult |
| description |
Purpose: The aim of this study was to investigate the influence of the TNF -308 G/A polymorphism in the promoter region of the tumor necrosis factor- α gene on the susceptibility and severity of rheumatoid arthritis (RA) in individuals from the Brazilian Amazon. Methods: A total of 323 individuals-192 healthy controls without arthritis and 131 individuals suffering from arthritis-were genotyped for this polymorphism using a methodology based on PCR-RFLP. Results: The frequency of the A allele (TNF2) in rheumatoid arthritis sufferers was not significantly higher than in the controls (p= 0.926; OR = 0.97; confidence interval 0.54-1.76). However, using a logistic regression model, when the patients were stratified according to whether the manifestations were preponderantly articular or systemic, there was a strong association between the TNF2 allele and systemic arthritis (p= 0.001; OR = 5.89; confidence interval = 1.98-17.5) as well as the use of anti-TNF immunotherapy (p= 0.023; OR = 1.10; confidence interval = 1.00-1.14). The main factors that were found to influence the risk of extra-articular disease were age greater than or equal to 60. years (p= 0.008; OR = 4.06; confidence interval = 1.45-11.38), disease duration greater than 10. years (p= 0.031; OR = 3.10; confidence interval = 1.11-8.63) and positive rheumatoid factor (p= 0.035; OR = 2.07; confidence interval = 1.05-4.09). Conclusions: These results suggest that the TNF2 allele is associated with the more serious forms of the disease in individuals from the Brazilian Amazon but not with a risk for developing RA. © 2012 Elsevier Ltd. |
| format |
Artigo |
| author |
Boechat, Antonio Luiz |
| author2 |
Boechat, Narjara de Oliveira Ogusku, Maurício Morishi Alencar, Mariana Raposo Abensur, Themis da Costa Neto, José Cardoso Amorim, Leilian de Souza Oliveira, Lívia Moura de Sadahiro, Aya Santos, Maria Cristina dos |
| author2Str |
Boechat, Narjara de Oliveira Ogusku, Maurício Morishi Alencar, Mariana Raposo Abensur, Themis da Costa Neto, José Cardoso Amorim, Leilian de Souza Oliveira, Lívia Moura de Sadahiro, Aya Santos, Maria Cristina dos |
| title |
The influence of a TNF gene polymorphism on the severity of rheumatoid arthritis in the Brazilian Amazon |
| title_short |
The influence of a TNF gene polymorphism on the severity of rheumatoid arthritis in the Brazilian Amazon |
| title_full |
The influence of a TNF gene polymorphism on the severity of rheumatoid arthritis in the Brazilian Amazon |
| title_fullStr |
The influence of a TNF gene polymorphism on the severity of rheumatoid arthritis in the Brazilian Amazon |
| title_full_unstemmed |
The influence of a TNF gene polymorphism on the severity of rheumatoid arthritis in the Brazilian Amazon |
| title_sort |
influence of a tnf gene polymorphism on the severity of rheumatoid arthritis in the brazilian amazon |
| publisher |
Cytokine |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/17912 |
| _version_ |
1787142766392770560 |
| score |
11.675088 |