Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil
As there are four major molecular types of Cryptococcus neoformans (VNI, VNII, VNIII and VNIV) and four molecular types of Cryptococcus gattii (VGI, VGII, VGIII and VGIV), it is important to identify the specific groups causing cryptococcosis in different geographical regions. Here, we investigated...
| Autor principal: | Freire, Ana Karla Lima L. |
|---|---|
| Outros Autores: | Bentes, Amaury dos Santos, Lima Sampaio, Ivanete de, Matsuura, Ani Beatriz Jackisch, Ogusku, Maurício Morishi, Salem, Júlia Ignez, Wanke, Bodo, Souza, João Vicente Braga de |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Mycoses
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/18047 |
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oai:repositorio:1-18047 |
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oai:repositorio:1-18047 Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil Freire, Ana Karla Lima L. Bentes, Amaury dos Santos Lima Sampaio, Ivanete de Matsuura, Ani Beatriz Jackisch Ogusku, Maurício Morishi Salem, Júlia Ignez Wanke, Bodo Souza, João Vicente Braga de Fungal Protein Unclassified Drug Ura5 Protein Adolescent Adult Brasil Cerebrospinal Fluid Controlled Study Cryptococcosis Cryptococcus Cryptococcus Gattii Cryptococcus Neoformans Dna Fingerprinting Female Fungal Gene Fungus Isolation Gene Expression Gene Locus Healthcare Facility Human Human Immunodeficiency Virus Human Immunodeficiency Virus Infection Major Clinical Study Male Molecular Genetics Polymerase Chain Reaction Prevalence Priority Journal Polymorphism, Restriction Fragment Length Risk Factor Species Diversity Tertiary Health Care Variable Number Of Tandem Repeat Virus Isolation Adolescent Adult Aged Ambulatory Care Facilities Brasil Child Child, Preschool Cryptococcosis Cryptococcus Female Humans Male Middle Aged Molecular Sequence Data Mycological Typing Techniques Polymorphism, Restriction Fragment Length Young Adult Cryptococcus Gattii Filobasidiella Neoformans As there are four major molecular types of Cryptococcus neoformans (VNI, VNII, VNIII and VNIV) and four molecular types of Cryptococcus gattii (VGI, VGII, VGIII and VGIV), it is important to identify the specific groups causing cryptococcosis in different geographical regions. Here, we investigated the molecular types of 57 cryptococcal isolates from patients in a tertiary care hospital in the state of Amazonas, Brazil, between 2006 and 2010. The isolates were characterised by PCR fingerprinting using the M13 minisatellite and confirmed by URA5-RFLP analysis, and the presence of specific genes from the mating type locus (MATα and MATa) of these species was analysed by PCR. Most of the patients were male (66.7%), between 16 and 30years of age (51.7%), and HIV-positive (75.0%). Most isolates were collected from cerebrospinal fluid samples (71.7%). Most of the C. neoformans isolates (n=40) were characterised as members of the VNI molecular group (n=39), a unique isolate was characterised as VNII whereas all isolates of C. gattii (n=17) were members of the VGII molecular group. With regard to mating types, 55 isolates were type 'α', and only two were type 'a'. This study revealed the prevalence of the VNI molecular group and provides the first reported observation of the VNII molecular group in the northern region of Brazil. © 2012 Blackwell Verlag GmbH. 2020-06-15T21:51:12Z 2020-06-15T21:51:12Z 2012 Artigo https://repositorio.inpa.gov.br/handle/1/18047 10.1111/j.1439-0507.2012.02173.x en Volume 55, Número 3, Pags. e145-e150 Restrito Mycoses |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Fungal Protein Unclassified Drug Ura5 Protein Adolescent Adult Brasil Cerebrospinal Fluid Controlled Study Cryptococcosis Cryptococcus Cryptococcus Gattii Cryptococcus Neoformans Dna Fingerprinting Female Fungal Gene Fungus Isolation Gene Expression Gene Locus Healthcare Facility Human Human Immunodeficiency Virus Human Immunodeficiency Virus Infection Major Clinical Study Male Molecular Genetics Polymerase Chain Reaction Prevalence Priority Journal Polymorphism, Restriction Fragment Length Risk Factor Species Diversity Tertiary Health Care Variable Number Of Tandem Repeat Virus Isolation Adolescent Adult Aged Ambulatory Care Facilities Brasil Child Child, Preschool Cryptococcosis Cryptococcus Female Humans Male Middle Aged Molecular Sequence Data Mycological Typing Techniques Polymorphism, Restriction Fragment Length Young Adult Cryptococcus Gattii Filobasidiella Neoformans |
| spellingShingle |
Fungal Protein Unclassified Drug Ura5 Protein Adolescent Adult Brasil Cerebrospinal Fluid Controlled Study Cryptococcosis Cryptococcus Cryptococcus Gattii Cryptococcus Neoformans Dna Fingerprinting Female Fungal Gene Fungus Isolation Gene Expression Gene Locus Healthcare Facility Human Human Immunodeficiency Virus Human Immunodeficiency Virus Infection Major Clinical Study Male Molecular Genetics Polymerase Chain Reaction Prevalence Priority Journal Polymorphism, Restriction Fragment Length Risk Factor Species Diversity Tertiary Health Care Variable Number Of Tandem Repeat Virus Isolation Adolescent Adult Aged Ambulatory Care Facilities Brasil Child Child, Preschool Cryptococcosis Cryptococcus Female Humans Male Middle Aged Molecular Sequence Data Mycological Typing Techniques Polymorphism, Restriction Fragment Length Young Adult Cryptococcus Gattii Filobasidiella Neoformans Freire, Ana Karla Lima L. Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil |
| topic_facet |
Fungal Protein Unclassified Drug Ura5 Protein Adolescent Adult Brasil Cerebrospinal Fluid Controlled Study Cryptococcosis Cryptococcus Cryptococcus Gattii Cryptococcus Neoformans Dna Fingerprinting Female Fungal Gene Fungus Isolation Gene Expression Gene Locus Healthcare Facility Human Human Immunodeficiency Virus Human Immunodeficiency Virus Infection Major Clinical Study Male Molecular Genetics Polymerase Chain Reaction Prevalence Priority Journal Polymorphism, Restriction Fragment Length Risk Factor Species Diversity Tertiary Health Care Variable Number Of Tandem Repeat Virus Isolation Adolescent Adult Aged Ambulatory Care Facilities Brasil Child Child, Preschool Cryptococcosis Cryptococcus Female Humans Male Middle Aged Molecular Sequence Data Mycological Typing Techniques Polymorphism, Restriction Fragment Length Young Adult Cryptococcus Gattii Filobasidiella Neoformans |
| description |
As there are four major molecular types of Cryptococcus neoformans (VNI, VNII, VNIII and VNIV) and four molecular types of Cryptococcus gattii (VGI, VGII, VGIII and VGIV), it is important to identify the specific groups causing cryptococcosis in different geographical regions. Here, we investigated the molecular types of 57 cryptococcal isolates from patients in a tertiary care hospital in the state of Amazonas, Brazil, between 2006 and 2010. The isolates were characterised by PCR fingerprinting using the M13 minisatellite and confirmed by URA5-RFLP analysis, and the presence of specific genes from the mating type locus (MATα and MATa) of these species was analysed by PCR. Most of the patients were male (66.7%), between 16 and 30years of age (51.7%), and HIV-positive (75.0%). Most isolates were collected from cerebrospinal fluid samples (71.7%). Most of the C. neoformans isolates (n=40) were characterised as members of the VNI molecular group (n=39), a unique isolate was characterised as VNII whereas all isolates of C. gattii (n=17) were members of the VGII molecular group. With regard to mating types, 55 isolates were type 'α', and only two were type 'a'. This study revealed the prevalence of the VNI molecular group and provides the first reported observation of the VNII molecular group in the northern region of Brazil. © 2012 Blackwell Verlag GmbH. |
| format |
Artigo |
| author |
Freire, Ana Karla Lima L. |
| author2 |
Bentes, Amaury dos Santos Lima Sampaio, Ivanete de Matsuura, Ani Beatriz Jackisch Ogusku, Maurício Morishi Salem, Júlia Ignez Wanke, Bodo Souza, João Vicente Braga de |
| author2Str |
Bentes, Amaury dos Santos Lima Sampaio, Ivanete de Matsuura, Ani Beatriz Jackisch Ogusku, Maurício Morishi Salem, Júlia Ignez Wanke, Bodo Souza, João Vicente Braga de |
| title |
Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil |
| title_short |
Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil |
| title_full |
Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil |
| title_fullStr |
Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil |
| title_full_unstemmed |
Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil |
| title_sort |
molecular characterisation of the causative agents of cryptococcosis in patients of a tertiary healthcare facility in the state of amazonas-brazil |
| publisher |
Mycoses |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/18047 |
| _version_ |
1787141934227128320 |
| score |
11.653393 |