Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds
A lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour-diffusion method using a precipitant solution consisting of 0.1 M Tris-HCl pH 7.8, 8%(w/v) PEG 3350 and 0.2 M proline at a constant temperature o...
| Autor principal: | Cavada, B. S. |
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| Outros Autores: | Marinho, Emmanuel S., Souza, Emmanuel Prata, Benevides, R. G., Delatorre, Plínio, Souza, Luis A.G., Nascimento, K. S., Sampaio, Alexandre Holanda, Moreno, Frederico Bruno Mendes Batista, Rustiguel, Joane Kathelen Rodrigues, Canduri, Fernanda, Azevedo, Walter Filgueira de, Debray, Henri |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Acta Crystallographica Section F: Structural Biology and Crystallization Communications
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/18792 |
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oai:repositorio:1-18792 |
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oai:repositorio:1-18792 Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds Cavada, B. S. Marinho, Emmanuel S. Souza, Emmanuel Prata Benevides, R. G. Delatorre, Plínio Souza, Luis A.G. Nascimento, K. S. Sampaio, Alexandre Holanda Moreno, Frederico Bruno Mendes Batista Rustiguel, Joane Kathelen Rodrigues Canduri, Fernanda Azevedo, Walter Filgueira de Debray, Henri Cymbosema Roseum Mannose Plant Lectin Chromatography, Affinity Amino Acid Sequence Animals Chemistry Crystallization Hemagglutination Isolation And Purification Legume Methodology Molecular Genetics Seed Plant Rabbit X Ray Crystallography Amino Acid Sequence Animal Chromatography, Affinity Crystallization Crystallography, X-ray Fabaceae Hemagglutination Mannose Molecular Sequence Data Plant Lectins Rabbits Seeds A lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour-diffusion method using a precipitant solution consisting of 0.1 M Tris-HCl pH 7.8, 8%(w/v) PEG 3350 and 0.2 M proline at a constant temperature of 293 K. A data set was collected to 1.77 Å resolution at a synchrotron-radiation source. CRL crystals are orthorhombic, belonging to space group P212121. Crystallographic refinement and full amino-acid sequence determination are in progress. © 2006 International Union of Crystallography. All rights reserved. 2020-06-15T22:03:04Z 2020-06-15T22:03:04Z 2006 Artigo https://repositorio.inpa.gov.br/handle/1/18792 10.1107/S174430910600371X en Volume 62, Número 3, Pags. 235-237 Restrito Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Cymbosema Roseum Mannose Plant Lectin Chromatography, Affinity Amino Acid Sequence Animals Chemistry Crystallization Hemagglutination Isolation And Purification Legume Methodology Molecular Genetics Seed Plant Rabbit X Ray Crystallography Amino Acid Sequence Animal Chromatography, Affinity Crystallization Crystallography, X-ray Fabaceae Hemagglutination Mannose Molecular Sequence Data Plant Lectins Rabbits Seeds |
| spellingShingle |
Cymbosema Roseum Mannose Plant Lectin Chromatography, Affinity Amino Acid Sequence Animals Chemistry Crystallization Hemagglutination Isolation And Purification Legume Methodology Molecular Genetics Seed Plant Rabbit X Ray Crystallography Amino Acid Sequence Animal Chromatography, Affinity Crystallization Crystallography, X-ray Fabaceae Hemagglutination Mannose Molecular Sequence Data Plant Lectins Rabbits Seeds Cavada, B. S. Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds |
| topic_facet |
Cymbosema Roseum Mannose Plant Lectin Chromatography, Affinity Amino Acid Sequence Animals Chemistry Crystallization Hemagglutination Isolation And Purification Legume Methodology Molecular Genetics Seed Plant Rabbit X Ray Crystallography Amino Acid Sequence Animal Chromatography, Affinity Crystallization Crystallography, X-ray Fabaceae Hemagglutination Mannose Molecular Sequence Data Plant Lectins Rabbits Seeds |
| description |
A lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour-diffusion method using a precipitant solution consisting of 0.1 M Tris-HCl pH 7.8, 8%(w/v) PEG 3350 and 0.2 M proline at a constant temperature of 293 K. A data set was collected to 1.77 Å resolution at a synchrotron-radiation source. CRL crystals are orthorhombic, belonging to space group P212121. Crystallographic refinement and full amino-acid sequence determination are in progress. © 2006 International Union of Crystallography. All rights reserved. |
| format |
Artigo |
| author |
Cavada, B. S. |
| author2 |
Marinho, Emmanuel S. Souza, Emmanuel Prata Benevides, R. G. Delatorre, Plínio Souza, Luis A.G. Nascimento, K. S. Sampaio, Alexandre Holanda Moreno, Frederico Bruno Mendes Batista Rustiguel, Joane Kathelen Rodrigues Canduri, Fernanda Azevedo, Walter Filgueira de Debray, Henri |
| author2Str |
Marinho, Emmanuel S. Souza, Emmanuel Prata Benevides, R. G. Delatorre, Plínio Souza, Luis A.G. Nascimento, K. S. Sampaio, Alexandre Holanda Moreno, Frederico Bruno Mendes Batista Rustiguel, Joane Kathelen Rodrigues Canduri, Fernanda Azevedo, Walter Filgueira de Debray, Henri |
| title |
Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds |
| title_short |
Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds |
| title_full |
Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds |
| title_fullStr |
Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds |
| title_full_unstemmed |
Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds |
| title_sort |
purification, partial characterization and preliminary x-ray diffraction analysis of a mannose-specific lectin from cymbosema roseum seeds |
| publisher |
Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/18792 |
| _version_ |
1787142439049363456 |
| score |
11.755432 |