Production of xylan-degrading enzymes from Amazon forest fungal species
Ten fungal species were isolated from decomposing wood in the Amazon forest. All produced xylan-degrading enzymes when cultivated in liquid media containing oat spelt xylan. The best producing strains were identified as Penicillium corylophilum, Aspergillus niger and Trichoderma longibrachiatum. The...
| Autor principal: | Garcia Medeiros, Roseli |
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| Outros Autores: | Hanada, Rogério Eiji, Filho, Edivaldo Ximenes Ferreira |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
International Biodeterioration and Biodegradation
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/19007 |
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oai:repositorio:1-19007 |
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oai:repositorio:1-19007 Production of xylan-degrading enzymes from Amazon forest fungal species Garcia Medeiros, Roseli Hanada, Rogério Eiji Filho, Edivaldo Ximenes Ferreira Catalyst Activity Forestry Fungi Solubility Wood Xylans Enzymes Dead Wood Decomposition Enzyme Fungus Rainforest Amazonia South America Aspergillus Aspergillus Niger Aspergillus Niger Fungi Hypocrea Lixii Penicillium Penicillium Corylophilum Penicillium Corylophilum Trichoderma Trichoderma Harzianum Trichoderma Longibrachiatum Trichoderma Longibrachiatum Triticum Aestivum Subsp. Spelta Ten fungal species were isolated from decomposing wood in the Amazon forest. All produced xylan-degrading enzymes when cultivated in liquid media containing oat spelt xylan. The best producing strains were identified as Penicillium corylophilum, Aspergillus niger and Trichoderma longibrachiatum. The best yields of β-xylosidase and α-arabinofuranosidase activities were Trichoderma harzianum and Trichoderma sp. Xylanase activities from crude extract samples of P. corylophilum, A. niger and T. longibrachiatum were partially characterized. They were most active at 40°C (A. niger) or 45°C (P. corylophilum and T. longibrachiatum) and pH 4.0-4.5. Reducing agents (β-mercaptoethanol and dithiothreitol), L-cysteine and L-tryptophan activated xylanase activity. In addition, dithiothreitol improved the half-lives of these enzymes at 50°C and 60°C. By contrast, N-bromosuccinimide inhibited all the enzyme activities. Xylan and dithiothreitol afforded protection against xylan-degrading enzyme inactivation by N-bromosuccinimide, but failed to reverse it. The apparent Km values on soluble and insoluble xylans from oat spelt showed that xylan-degrading enzymes from A. niger, T. longibrachiatum and P. corylophilum were most active on the soluble form. © 2003 Elsevier Ltd. All rights reserved. 2020-06-15T22:04:33Z 2020-06-15T22:04:33Z 2003 Artigo https://repositorio.inpa.gov.br/handle/1/19007 10.1016/S0964-8305(02)00179-8 en Volume 52, Número 2, Pags. 97-100 Restrito International Biodeterioration and Biodegradation |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Catalyst Activity Forestry Fungi Solubility Wood Xylans Enzymes Dead Wood Decomposition Enzyme Fungus Rainforest Amazonia South America Aspergillus Aspergillus Niger Aspergillus Niger Fungi Hypocrea Lixii Penicillium Penicillium Corylophilum Penicillium Corylophilum Trichoderma Trichoderma Harzianum Trichoderma Longibrachiatum Trichoderma Longibrachiatum Triticum Aestivum Subsp. Spelta |
| spellingShingle |
Catalyst Activity Forestry Fungi Solubility Wood Xylans Enzymes Dead Wood Decomposition Enzyme Fungus Rainforest Amazonia South America Aspergillus Aspergillus Niger Aspergillus Niger Fungi Hypocrea Lixii Penicillium Penicillium Corylophilum Penicillium Corylophilum Trichoderma Trichoderma Harzianum Trichoderma Longibrachiatum Trichoderma Longibrachiatum Triticum Aestivum Subsp. Spelta Garcia Medeiros, Roseli Production of xylan-degrading enzymes from Amazon forest fungal species |
| topic_facet |
Catalyst Activity Forestry Fungi Solubility Wood Xylans Enzymes Dead Wood Decomposition Enzyme Fungus Rainforest Amazonia South America Aspergillus Aspergillus Niger Aspergillus Niger Fungi Hypocrea Lixii Penicillium Penicillium Corylophilum Penicillium Corylophilum Trichoderma Trichoderma Harzianum Trichoderma Longibrachiatum Trichoderma Longibrachiatum Triticum Aestivum Subsp. Spelta |
| description |
Ten fungal species were isolated from decomposing wood in the Amazon forest. All produced xylan-degrading enzymes when cultivated in liquid media containing oat spelt xylan. The best producing strains were identified as Penicillium corylophilum, Aspergillus niger and Trichoderma longibrachiatum. The best yields of β-xylosidase and α-arabinofuranosidase activities were Trichoderma harzianum and Trichoderma sp. Xylanase activities from crude extract samples of P. corylophilum, A. niger and T. longibrachiatum were partially characterized. They were most active at 40°C (A. niger) or 45°C (P. corylophilum and T. longibrachiatum) and pH 4.0-4.5. Reducing agents (β-mercaptoethanol and dithiothreitol), L-cysteine and L-tryptophan activated xylanase activity. In addition, dithiothreitol improved the half-lives of these enzymes at 50°C and 60°C. By contrast, N-bromosuccinimide inhibited all the enzyme activities. Xylan and dithiothreitol afforded protection against xylan-degrading enzyme inactivation by N-bromosuccinimide, but failed to reverse it. The apparent Km values on soluble and insoluble xylans from oat spelt showed that xylan-degrading enzymes from A. niger, T. longibrachiatum and P. corylophilum were most active on the soluble form. © 2003 Elsevier Ltd. All rights reserved. |
| format |
Artigo |
| author |
Garcia Medeiros, Roseli |
| author2 |
Hanada, Rogério Eiji Filho, Edivaldo Ximenes Ferreira |
| author2Str |
Hanada, Rogério Eiji Filho, Edivaldo Ximenes Ferreira |
| title |
Production of xylan-degrading enzymes from Amazon forest fungal species |
| title_short |
Production of xylan-degrading enzymes from Amazon forest fungal species |
| title_full |
Production of xylan-degrading enzymes from Amazon forest fungal species |
| title_fullStr |
Production of xylan-degrading enzymes from Amazon forest fungal species |
| title_full_unstemmed |
Production of xylan-degrading enzymes from Amazon forest fungal species |
| title_sort |
production of xylan-degrading enzymes from amazon forest fungal species |
| publisher |
International Biodeterioration and Biodegradation |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/19007 |
| _version_ |
1787143198070538240 |
| score |
11.755432 |