Artigo

Quantitative evaluation of acidity tolerance of root nodule bacteria

Quantification of acidity tolerance in the laboratory may be the first step in rhizobial strain selection for the Amazon region. The present method evaluated rhizobia in Petri dishes with YMA medium at pH 6.5 (control) and 4.5, using scores of 1.0 (sensitive, 'no visible' growth) to 4.0 (tolerant, m...

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Autor principal: Oliveira, Luiz Antonio de
Outros Autores: Magalhaes, H. P. de
Grau: Artigo
Idioma: English
Publicado em: Revista de Microbiologia 2020
Acesso em linha: https://repositorio.inpa.gov.br/handle/1/19192
id oai:repositorio:1-19192
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spelling oai:repositorio:1-19192 Quantitative evaluation of acidity tolerance of root nodule bacteria Oliveira, Luiz Antonio de Magalhaes, H. P. de Quantification of acidity tolerance in the laboratory may be the first step in rhizobial strain selection for the Amazon region. The present method evaluated rhizobia in Petri dishes with YMA medium at pH 6.5 (control) and 4.5, using scores of 1.0 (sensitive, 'no visible' growth) to 4.0 (tolerant, maximum growth). Growth evaluations were done at 6, 9, 12, 15 and 18 day periods. This method permits preliminary selection of root nodule bacteria from Amazonian soils with statistical precision. Among the 31 rhizobia strains initially tested, the INPA strains 048,078, and 671 presented scores of 4.0 at both pHs after 9 days of growth. Strain analyses using a less rigorous criterion (growth scores higher than 3.0) included in this highly tolerant group the INPA strains 511, 565, 576, 632, 649, and 658, which grew on the most diluted zone (zone 4) after 9 days. Tolerant strains still must be tested for nitrogen fixation effectiveness, competitiveness for nodule sites, and soil persistence before their recommendation as inoculants. 2020-06-15T22:06:11Z 2020-06-15T22:06:11Z 1999 Artigo https://repositorio.inpa.gov.br/handle/1/19192 en Volume 30, Número 3, Pags. 203-208 Restrito Revista de Microbiologia
institution Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional
collection INPA-RI
language English
description Quantification of acidity tolerance in the laboratory may be the first step in rhizobial strain selection for the Amazon region. The present method evaluated rhizobia in Petri dishes with YMA medium at pH 6.5 (control) and 4.5, using scores of 1.0 (sensitive, 'no visible' growth) to 4.0 (tolerant, maximum growth). Growth evaluations were done at 6, 9, 12, 15 and 18 day periods. This method permits preliminary selection of root nodule bacteria from Amazonian soils with statistical precision. Among the 31 rhizobia strains initially tested, the INPA strains 048,078, and 671 presented scores of 4.0 at both pHs after 9 days of growth. Strain analyses using a less rigorous criterion (growth scores higher than 3.0) included in this highly tolerant group the INPA strains 511, 565, 576, 632, 649, and 658, which grew on the most diluted zone (zone 4) after 9 days. Tolerant strains still must be tested for nitrogen fixation effectiveness, competitiveness for nodule sites, and soil persistence before their recommendation as inoculants.
format Artigo
author Oliveira, Luiz Antonio de
spellingShingle Oliveira, Luiz Antonio de
Quantitative evaluation of acidity tolerance of root nodule bacteria
author2 Magalhaes, H. P. de
author2Str Magalhaes, H. P. de
title Quantitative evaluation of acidity tolerance of root nodule bacteria
title_short Quantitative evaluation of acidity tolerance of root nodule bacteria
title_full Quantitative evaluation of acidity tolerance of root nodule bacteria
title_fullStr Quantitative evaluation of acidity tolerance of root nodule bacteria
title_full_unstemmed Quantitative evaluation of acidity tolerance of root nodule bacteria
title_sort quantitative evaluation of acidity tolerance of root nodule bacteria
publisher Revista de Microbiologia
publishDate 2020
url https://repositorio.inpa.gov.br/handle/1/19192
_version_ 1787144559221800960
score 11.755432

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