Paracoccidioides brasiliensis isolated from armadillos is virulent to Syrian hamsters
Isolates of Paracoccidioides brasiliensis may vary in virulence according to time of in vitro subcultivation. The present study compared the morphology and pathogenicity to hamsters of two P. brasiliensis isolates: one obtained from human lesions and maintained in the laboratory for several years (P...
| Autor principal: | Pera?oli, Maria Terezinha Serrão |
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| Outros Autores: | Sugizaki, Maria Fátima, Mendes, Rinaldo Pôncio, Naiff, Roberto Daibes, Montenegro, Mário Rubens Guimarães |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Mycopathologia
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/19199 |
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Isolates of Paracoccidioides brasiliensis may vary in virulence according to time of in vitro subcultivation. The present study compared the morphology and pathogenicity to hamsters of two P. brasiliensis isolates: one obtained from human lesions and maintained in the laboratory for several years (Pb-18) and the other isolate recovered from hamsters inoculated with organ homogenates from armadillos (Pb-T). The microscopic morphology of Pb-18 and Pb-T showed yeast cells with similar diameter. However, Pb-T produced a significantly higher number of buds per mother cell than Pb-18. Besides, the mycelial form of Pb-T developed abundant sporulation during 8 weeks of culture which was absent in the Pb-18 isolate. Virulence studies demonstrated that mortality rates, antibody levels, fungal load and extent of lesions in the organs were significantly higher in animals infected with Pb-T. The results demonstrated that Pb-T recently isolated from an animal was more virulent than Pb-18. These differences between the two P. brasillensis isolates may be indicators of virulence attenuation in this fungal species. |