Paracoccidioides brasiliensis isolated from armadillos is virulent to Syrian hamsters
Isolates of Paracoccidioides brasiliensis may vary in virulence according to time of in vitro subcultivation. The present study compared the morphology and pathogenicity to hamsters of two P. brasiliensis isolates: one obtained from human lesions and maintained in the laboratory for several years (P...
| Autor principal: | Pera?oli, Maria Terezinha Serrão |
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| Outros Autores: | Sugizaki, Maria Fátima, Mendes, Rinaldo Pôncio, Naiff, Roberto Daibes, Montenegro, Mário Rubens Guimarães |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Mycopathologia
2020
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| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/19199 |
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oai:repositorio:1-19199 |
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oai:repositorio:1-19199 Paracoccidioides brasiliensis isolated from armadillos is virulent to Syrian hamsters Pera?oli, Maria Terezinha Serrão Sugizaki, Maria Fátima Mendes, Rinaldo Pôncio Naiff, Roberto Daibes Montenegro, Mário Rubens Guimarães Fungus Antibody Animals Armadillo Blood Female Hamster Human Immunology Lung Male Microbiology Paracoccidioides Pathogenicity South American Blastomycosis Syrian Hamster Virulence Animal Antibodies, Fungal Armadillos Cricetinae Female Humans Lung Male Mesocricetus Paracoccidioides Paracoccidioidomycosis Virulence Animalsia Armadillo Cricetinae Dasypodidae Fungi Mesocricetus Auratus Paracoccidioides Brasiliensis Isolates of Paracoccidioides brasiliensis may vary in virulence according to time of in vitro subcultivation. The present study compared the morphology and pathogenicity to hamsters of two P. brasiliensis isolates: one obtained from human lesions and maintained in the laboratory for several years (Pb-18) and the other isolate recovered from hamsters inoculated with organ homogenates from armadillos (Pb-T). The microscopic morphology of Pb-18 and Pb-T showed yeast cells with similar diameter. However, Pb-T produced a significantly higher number of buds per mother cell than Pb-18. Besides, the mycelial form of Pb-T developed abundant sporulation during 8 weeks of culture which was absent in the Pb-18 isolate. Virulence studies demonstrated that mortality rates, antibody levels, fungal load and extent of lesions in the organs were significantly higher in animals infected with Pb-T. The results demonstrated that Pb-T recently isolated from an animal was more virulent than Pb-18. These differences between the two P. brasillensis isolates may be indicators of virulence attenuation in this fungal species. 2020-06-15T22:06:15Z 2020-06-15T22:06:15Z 1999 Artigo https://repositorio.inpa.gov.br/handle/1/19199 10.1023/A:1007119403572 en Volume 148, Número 3, Pags. 123-130 Restrito Mycopathologia |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Fungus Antibody Animals Armadillo Blood Female Hamster Human Immunology Lung Male Microbiology Paracoccidioides Pathogenicity South American Blastomycosis Syrian Hamster Virulence Animal Antibodies, Fungal Armadillos Cricetinae Female Humans Lung Male Mesocricetus Paracoccidioides Paracoccidioidomycosis Virulence Animalsia Armadillo Cricetinae Dasypodidae Fungi Mesocricetus Auratus Paracoccidioides Brasiliensis |
| spellingShingle |
Fungus Antibody Animals Armadillo Blood Female Hamster Human Immunology Lung Male Microbiology Paracoccidioides Pathogenicity South American Blastomycosis Syrian Hamster Virulence Animal Antibodies, Fungal Armadillos Cricetinae Female Humans Lung Male Mesocricetus Paracoccidioides Paracoccidioidomycosis Virulence Animalsia Armadillo Cricetinae Dasypodidae Fungi Mesocricetus Auratus Paracoccidioides Brasiliensis Pera?oli, Maria Terezinha Serrão Paracoccidioides brasiliensis isolated from armadillos is virulent to Syrian hamsters |
| topic_facet |
Fungus Antibody Animals Armadillo Blood Female Hamster Human Immunology Lung Male Microbiology Paracoccidioides Pathogenicity South American Blastomycosis Syrian Hamster Virulence Animal Antibodies, Fungal Armadillos Cricetinae Female Humans Lung Male Mesocricetus Paracoccidioides Paracoccidioidomycosis Virulence Animalsia Armadillo Cricetinae Dasypodidae Fungi Mesocricetus Auratus Paracoccidioides Brasiliensis |
| description |
Isolates of Paracoccidioides brasiliensis may vary in virulence according to time of in vitro subcultivation. The present study compared the morphology and pathogenicity to hamsters of two P. brasiliensis isolates: one obtained from human lesions and maintained in the laboratory for several years (Pb-18) and the other isolate recovered from hamsters inoculated with organ homogenates from armadillos (Pb-T). The microscopic morphology of Pb-18 and Pb-T showed yeast cells with similar diameter. However, Pb-T produced a significantly higher number of buds per mother cell than Pb-18. Besides, the mycelial form of Pb-T developed abundant sporulation during 8 weeks of culture which was absent in the Pb-18 isolate. Virulence studies demonstrated that mortality rates, antibody levels, fungal load and extent of lesions in the organs were significantly higher in animals infected with Pb-T. The results demonstrated that Pb-T recently isolated from an animal was more virulent than Pb-18. These differences between the two P. brasillensis isolates may be indicators of virulence attenuation in this fungal species. |
| format |
Artigo |
| author |
Pera?oli, Maria Terezinha Serrão |
| author2 |
Sugizaki, Maria Fátima Mendes, Rinaldo Pôncio Naiff, Roberto Daibes Montenegro, Mário Rubens Guimarães |
| author2Str |
Sugizaki, Maria Fátima Mendes, Rinaldo Pôncio Naiff, Roberto Daibes Montenegro, Mário Rubens Guimarães |
| title |
Paracoccidioides brasiliensis isolated from armadillos is virulent to Syrian hamsters |
| title_short |
Paracoccidioides brasiliensis isolated from armadillos is virulent to Syrian hamsters |
| title_full |
Paracoccidioides brasiliensis isolated from armadillos is virulent to Syrian hamsters |
| title_fullStr |
Paracoccidioides brasiliensis isolated from armadillos is virulent to Syrian hamsters |
| title_full_unstemmed |
Paracoccidioides brasiliensis isolated from armadillos is virulent to Syrian hamsters |
| title_sort |
paracoccidioides brasiliensis isolated from armadillos is virulent to syrian hamsters |
| publisher |
Mycopathologia |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/19199 |
| _version_ |
1787142116215881728 |
| score |
11.755432 |